ANTIBACTERIAL ACTIVTY OF BLACK MULBERRY (Morus nigra) FRUITS AND LEAVES

Öz

Antibacterial activities of the methanol and water extracts obtained from fruits and leaves of black mulberry (Morus nigra) were tested against 77 clinical strains of 5 bacteria species (Enterobacter aerogenes, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Staphylococcus aureus) by disc-diffusion method and minimal inhibitory concentration (MIC) values of each active extract were determined. The black mulberry extracts proved to be active against 4 of the 5 bacteria species tested in this study. The highest antibacterial activity was exhibited by methanol extract of mulberry leaves against S. aureus with 18 mm inhibition zone and 0.156 mg/ml MIC value. Also good antibacterial potentials were detected against E. aerogenes, E. coli, and P. aeruginosa with these extracts. Black mulberry extracts did not show any antibacterial activity against P. mirabilis.

Anahtar Kelimeler

• Antibakteriyel etki, Disk difüzyon yöntemi, Karadut (Morus nigra)

Kaynakça

1. Asono, N., Yamashita, T., Yasuda, K., Ikeda, K., Kizu, H., Kameda, Y. (2001). Polyhydroxylated alkoloids isolated from mulberry trees and silkworms. J Agric. Food Chem. 49, 4280–4213.
2. Baytop, T. (1999). Türkiyede bitkilerle tedavi. İstanbul Eczacılık Fakültesi Yayınları, İstanbul, pp. 444 (In Türkish).
3. Davis, P.H. (1982). Flora of Turkey and the East Agean Island. Edinburgh University Pres, Edinburgh, pp.642.
4. Ercişli S., Orhan E. (2007). Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food Chem. 103, 1380-1384.
5. Fukai, T., Kiyoshi, K., Terada S. (2005). Antimicrobial activity of 2arylbenzofurans from Morus species against methicillin-resistant Staphylococcus aureus. Fitoterapia, 76, 708–711.
6. Gerasopoulos, D., Stavorulakis, G. (1997). Quality characteristics of four mulberry (Morus sp.) cultivars in the area of China. J. Sci. Food Agric. 73, 261–264
7. Hammer, K.A., Carson, C.F., Riley, T.V. (1999). Antimicrobial activity of essential oils and other plant extracts. J Appl. Microbiol. 86, 985–990. Hodges, D.M., Kalt, W. (2003). Health functionality of small fruit. Acta Hort. 626, 17–23.
8. Kalt, W., Forney, C.F., Martin, A., Prior, R.L. (1999). Antioxidant capacity, vitamin C, phenolics, and anthocyanins after fresh storage of small fruits. J. Agric. Food Chem. 47, 4638–4644.

9. Kim, S.Y., Park, K.J., Lee, W.C. (1998). Antiinflamatory and antioxidative effects of Morus spp. fruit extracts. Korean J Med. Crop Sci. 41, 123– 128.
10. Kim, H.B., Bang, H.S., Lee, H.W., Seuk, Y.S., Sung, G.B. (1999). Chemical characteristics of mulberry syncarp. Korean J Med. Crop Sci. 47, 3206– 3209.
11. Krisch, J., Galgoczy, L., Tölgyesi, M., Papp, T., Vagvölgyi, C. (2008). Effect of fruit juices and pomace extracts on the growth of Gram-positive and Gram-negative bacteria. Acta Biol. Szeg. 52, 267–270.
12. Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C, Yolke, R.H. (1995). Manual Clinical Microbiology. ASM ,Washington , DC., pp.1356.
13. Park, K.M., You, J.S., Lee, H.Y., Baek, N.I., Hwang, N.I. (2003). Kuwanon G: an antibacterial agent from the root bark of Morus alba against oral pathogens. J. Ethnopharmacol. 84, 181–185.
14. Sohn, H.Y., Son, K.H., Kwon S., Kwon, G.S., Kang, S.S. (2004). Antimicrobial and cytotoxic activity of 18 prenylated favonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine. 11, 666–672.
15. Şengül, M., Ertugay, M.F., Şengül, M. (2005). Rheological, physical and chemical characteristics of mulberry pekmez. Food Control 16, 73–76.
16. Yiğit, N., Yiğit, D., Özgen, U., Aktaş, A. e. (2007). Anticandidal activity of black mulberry (Morus nigra). Türk Mikrobiyoloji Cemiyeti Dergisi 37, 169–173.
17. Zgoda, J.R., Porter, J.R. (2001). A convenient microdilition method for screening natural products against bacteria and fungi. Pharm. Biol. 39, 221–225.