Öz
In the present study, water and methanol extracts of hawthorn (Creatagus monogyna) were studied for antioxidant and antimicrobial properties. The antioxidant properties of fruits and leaves were evaluated by determining 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The water and methanol extracts of both fruits and leaves have antioxidant potential, ranging from 3.7 % to 44 %. Antimicrobial activities of above extracts were also tested against clinical isolates of human pathogenic strains belonging to 4 bacteria, Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and 3 yeast species, Candida albicans, Candida glabrata and Candida parapsilosis, by disk-diffusion method. The minimal inhibition concentration (MIC) value of each active extracts was also determined. The most effective antibacterial activity was expressed by methanol extract of hawthorn leaves against Gram-positive bacteria S. aureus with 12mm inhibition zone and 0.625 mg/ml MIC value. None of the extracts had antifungal activity against tested human pathogenic clinical isolates
Anahtar Kelimeler
Kaynakça
- Bahri-Sahloul R., Ben Fredj, R., Boughalleb N., Shriaa J., Saguem S., Hilbert J.,Francis T., Ammar,S., Bouzid, S. and Harzallah-Skhiri, F., 2014. Phenolic Composition and Antioxidant and AntimicrobialActivities of Extracts Obtained from Crataegus azarolus L. var.aronia (Willd.) Batt. Ovaries Calli. Hindawi Publishing Corporation Journal of Botany,1- 11.
- Baytop, T. (1999). Türkiyede bitkilerle tedavi. İstanbul Eczacılık Fakültesi Yayınları, İstanbul, pp.269, 146-147.
- Beecher, G.R., 1999. Phytonutrients role in metabolisim: Effects on resistance to degenerative processes. Nutrition Rev., 57, 3-6.
- Hodges D:M:, Kalt, W., 2003. Health functionality of small fruits. Acta Hort., 626, 17-23.
- Kostic, D. A, Velickovic, J. M , Mitic, S. S, Mitic, M.N.and Randelovic, S.S., 2012. Phenolic Content, and Antioxidant and Antimicrobial Activities of Crataegus Oxyacantha L (Rosaceae) Fruit Extract from Southeast Serbia. Tropical Journal of Pharmaceutical Research February. 11 (1): 117-124.
- Kumar, D., Arya V., Ali Bhat, Z., Khan, N., Prasad, D.N., 2012. The genus Crataegus: chemical and pharmacological perspectives. Brazilian Journal of Pharmacognosy 22(5): 1187-1200.
- Long, S. R., . Carey, R. A, Crofoot, K. M., Proteau, P. J., and Filtz, T. M., 2006. Effect of hawthorn (Crataegus oxycantha) crude extract and chromatographic culturedcardiomyocyte assay, Phytomedicine, vol. 13,no. 9-10, pp. on 643–650. multiple activities in a
- Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C and Yolke, R.H.,( 1995). Manual Clinical Microbiology. ASM, Washington , DC., pp.1356. Yiğit,D., Yiğit, N and Mavi A.,(2009). Antioxidant and antimicrobialactivitiesof bitter and sweet apricot kernels. Brazilian Journal of Medical and biological research 42(4):346-352.
- Zgoda, J.R. and Porter , J.R. (2001). A convenient microdilition method for screening natural products against bacteria and fungi. Pharmaceutical Biology, 39, 221–225.
SCREENING OF ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL OF HAWTHORN (CREATAGUS MONOGYNA) FRUIT AND LEAVES EXTRACTS
Öz
In the present study, water and methanol extracts of hawthorn (Creatagus monogyna) were studied for antioxidant and antimicrobial properties. The antioxidant properties of fruits and leaves were evaluated by determining DPPH radical scavenging activity. The water and methanol extracts of both fruits and leaves have antioxidant potential, ranging from 3.7 %to 44 %. Antimicrobial activities of above extracts were also tested against clinical isolates of human pathogenic strains belonging to 4 bacteria, Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and 3 yeast species, Candida albicans, Candida glabrata and Candida parapisilosis, by disc-diffusion method. The minimal inhibition concentration (MIC) value of each active extracts was also determined. The most effective antibacterial activity was expressed by methanol extract of hawthorn leaves against Gram-positive bacteria Staphylococcus aureus with 12mm inhibition zone and 0.625 mg/ml MIC value. None of the extracts have antifungal activity against tested human pathogen clinic isolates.
Keywords: Hawthorn (Creatagus monogyna), Antimicrobial activity, Antioxidant Activity
Anahtar Kelimeler
Alıç (Creatagus monogyna) Antimikrobiyal etki Antioksidant etki
Kaynakça
- Bahri-Sahloul R., Ben Fredj, R., Boughalleb N., Shriaa J., Saguem S., Hilbert J.,Francis T., Ammar,S., Bouzid, S. and Harzallah-Skhiri, F., 2014. Phenolic Composition and Antioxidant and AntimicrobialActivities of Extracts Obtained from Crataegus azarolus L. var.aronia (Willd.) Batt. Ovaries Calli. Hindawi Publishing Corporation Journal of Botany,1- 11.
- Baytop, T. (1999). Türkiyede bitkilerle tedavi. İstanbul Eczacılık Fakültesi Yayınları, İstanbul, pp.269, 146-147.
- Beecher, G.R., 1999. Phytonutrients role in metabolisim: Effects on resistance to degenerative processes. Nutrition Rev., 57, 3-6.
- Hodges D:M:, Kalt, W., 2003. Health functionality of small fruits. Acta Hort., 626, 17-23.
- Kostic, D. A, Velickovic, J. M , Mitic, S. S, Mitic, M.N.and Randelovic, S.S., 2012. Phenolic Content, and Antioxidant and Antimicrobial Activities of Crataegus Oxyacantha L (Rosaceae) Fruit Extract from Southeast Serbia. Tropical Journal of Pharmaceutical Research February. 11 (1): 117-124.
- Kumar, D., Arya V., Ali Bhat, Z., Khan, N., Prasad, D.N., 2012. The genus Crataegus: chemical and pharmacological perspectives. Brazilian Journal of Pharmacognosy 22(5): 1187-1200.
- Long, S. R., . Carey, R. A, Crofoot, K. M., Proteau, P. J., and Filtz, T. M., 2006. Effect of hawthorn (Crataegus oxycantha) crude extract and chromatographic culturedcardiomyocyte assay, Phytomedicine, vol. 13,no. 9-10, pp. on 643–650. multiple activities in a
- Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C and Yolke, R.H.,( 1995). Manual Clinical Microbiology. ASM, Washington , DC., pp.1356. Yiğit,D., Yiğit, N and Mavi A.,(2009). Antioxidant and antimicrobialactivitiesof bitter and sweet apricot kernels. Brazilian Journal of Medical and biological research 42(4):346-352.
- Zgoda, J.R. and Porter , J.R. (2001). A convenient microdilition method for screening natural products against bacteria and fungi. Pharmaceutical Biology, 39, 221–225.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 11 Ocak 2015 |
| Yayımlandığı Sayı | Yıl 2014 |