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Öz

Antibacterial activities of methanol and water extracts of blackberry (Rubus fruticosus) were tested against 63 clinical isolates of bacteria strains (Enterobacter aerogenes, Escherichia coli, Proteusmirabilis, Pseudomonas aeruginosa, and Staphylococcus aureus) by disc-diffusion method and minimal inhibition concentration (MIC) values of each active extract were determined. The blackberry extracts proved to be active against all bacteria species tested in this study. The highest antibacterial activity was expressed by methanol and aquouse extracts of blackberry fruits against S. aureus with 20 mm inhibition zone and 0.312 mg/ml MIC value. Also good antibacterial potentials were detected against Enterobacter aerogenes, Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa with methanol and water extracts of blackberry leaves ranging with 10-18mm inhibition zones

Anahtar Kelimeler

• Blackberry (Rubus fruticosus), Antibacterial activity, Disc diffusion method

ANTIBACTERIAL PROPERTIES OF BLACKBERRY (RUBUS FRUTICOSUS)

Öz

Antibacterial activities of methanol and water extracts of blackberry (Rubus fruticosus) were tested against 63 clinical isolates of bacteria strains (Enterobacter aerogenes, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Staphylococcus aureus) by disc-diffusion method and minimal inhibition concentration (MIC) values of each active extract were determined. The blackberry extracts proved to be active against all bacteria species tested in this study. The highest antibacterial activity was expressed by methanol and aquouse extracts of blackberry fruits against S. aureus with 20 mm inhibition zone and 0.312 mg/ml MIC value. Also good antibacterial potentials were detected against Enterobacter aerogenes, Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa with methanol and water extracts of blackberry leaves ranging with 10-18mm inhibition zones.

Keywords: Blackberry (Rubus fruticosus), Antibacterial activity, Disc diffusion method.

Anahtar Kelimeler

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Kaynakça

1. Abachi, S., Khademi, F.,Fatemi, H. and Malekzadef F.,2013. Study on antibacterial activity of selected Iranian plant extracts on Helicobacter pylori. IOSR Journal of Dental and Medical Sciences, Volume 5, Issue 1,55-59.
2. Anonymous, 2014. Health from nature, Remedies and natural cures. Available on link: 01.05.2014] [accessed date:
3. Baytop T. Türkiyede bitkilerle tedavi.İstanbul Eczacılık Fakültesi Yayınları, İstanbul, 1999, p.171.
4. Bobinaite, R., Viškelis, P. and Rimantas-Venskutonis, P.R., 2012. Variation of total phenolics,anthocyanins, ellagic acid and radical scavenging capacity in various raspberry(Rubus spp.) cultivars. Food Chem. 132,1495-1501.
5. Kim, T.K. (2012). Edible Medical and Non-medicinal Plants: Volume 4, Fruits. Springer, 2012, p. 545-548.
6. Krisch J., Galgoczy L., Papp T. And Vagvölgyı C., 2009.Antimicrobial and antioxidant potential of waste products remaining after juice pressing. Journal of Engineering, Tome VII, F.4, 131-134.
7. Lee, J., Dossett, M. and Finn, C.E., 2012. Rubus fruit phenolic research: The good, the bad, and the confusing. Food Chem. 130, 785-796.
8. Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C and Yolke, R.H., 1995. Manual Clinical Microbiology. ASM ,Washington , DC., pp.1356.

9. Oksman-Caldentey, K.M. and Puupponen-Pimiä, R.H., 2006. Berry Phenolics:AntimicrobialProperties and Mechanisms of Action Against Severe Human Pathogens. Nutr. Cancer. 54, 18-32.
10. Ördögh, L., Galgóczy, L., Krisch, J., Papp, T. and Vágvölgyi, C., 2010. Antioxidant and antimicrobialactivities of fruit juices and pomace extracts against acne-inducing bacteria. Acta Biologica Szegediensis 54, 1, 45-49.
11. Riaz,M., Ahmad,M.and Rahma, N.,2011. Antimicrobial screening of fruit, leaves, root and stem of Rubus fruticosus. Journal of Medicinal Plants Research, Vol 5(24), 5920-5924.
12. Velicanski, A.S., Cuetkovic D.D. and Markov,S.L., 2012. Screening of antibacterialactivity of raspberry fruit and pomace extracts. APTEFF,43, 305-313.
13. Zgoda, J.R.and Porter, J.R., 2001. A convenient microdilition method for screening natural products against bacteria and fungi. Pharmaceut. Biol. 39, 221–225.