Düşük Dozda N-Asetilsistein Uygulaması Diyabetik Yara İyileşmesini Hızlandırır mı?

Öz

Oksidatif stres ve kalıcı inflamasyon, diyabetik yara komplikasyonlarının gelişmesinde önemli rol oynar. N-Asetilsistein (NAC), antioksidan özelliklerinden dolayı yara iyileşmesini hızlandıran bir amino asit türevidir. Bu çalışmanın amacı, sistemik NAC uygulamasının diyabetik yara iyileşmesinde oksidatif stres, inflamatuar belirteçler ve kollajen üretimi üzerindeki etkisini değerlendirmektir. Wistar sıçanlarda diyabet indüksiyonu, streptozotosinin intraperitoneal olarak uygulanmasıyla yapılmıştır. Diyabetik sıçanlarda dorsal dairesel bir yara oluşturulmuştur. NAC'nin yara iyileştirme potansiyelini değerlendirmek için denekler intraperitoneal olarak NAC (60 mg / kg) ile tedavi edilmiştir. Diyabetik sıçanların yara dokularında lipid peroksidasyonu ve inflamatuar belirteçler önemli ölçüde artmıştır (p<0.05). Diyabetik grupta süperoksit dismutaz ve katalaz aktiviteleri, glutatyon ve NOx seviyeleri ve kollajen üretimi anlamlı olarak azalmıştır (p<0.05). NAC uygulaması, diyabetik gruba kıyasla, tedavi edilen diyabetik grupta antioksidan durumu ve NOx düzeylerini arttırmış, lipid peroksidasyonunu ve inflamatuar belirteç düzeylerini düşürmüştür (p<0.05). Ayrıca diyabetik NAC tedavi grubunda kollajen üretimi anlamlı olarak artmış ve yara alanı diyabetik gruba göre anlamlı olarak küçülmüştür (p<0.05). Düşük doz NAC uygulamasının, oksidatif stresi ve inflamasyonu azaltarak ve kollajen üretimini artırarak diyabetik yara iyileşmesinde etkili olduğu bulunmuştur.

Anahtar Kelimeler

• Yara iyileşmesi
• diyabet
• N-asetilsistein
• oksidatif stres
• pro-inflamatuar markörler

Proje Numarası

FEN-BAP-A-150219-66

Does Low Dose N-Acetylcysteine Administration Enhance Diabetic Wound Healing?

Öz

The aim of this study to establish the effect of systemic N-acetylcysteine (NAC) administration on the oxidative–antioxidative balance, inflammatory markers, and collagen production during wound healing in diabetes. A total of 24 male Wistar albino rats were randomly divided into 4 groups: control, streptozotocin-induced diabetic model, NAC treatment group, and streptozotocin-induced diabetic model with NAC treatment. The dorsal circular wound model was created in model rats and systemic NAC application (IP, 60 mg/kg) was performed for 7 days in rats in the treatment groups. Lipid peroxidation, antioxidative parameters, NOx levels, and amount of collagen in wound tissue were determined by spectrophotometric methods. Inflammatory markers of wound tissue were detected by ELISA. In the wound tissues of untreated diabetic rats, lipid peroxidation and inflammatory markers were significantly increased. Superoxide dismutase and catalase activities, glutathione and NOx levels, and collagen production were significantly reduced. Following systemic NAC administration, antioxidant status and NOx levels were significantly improved and lipid peroxidation and inflammatory marker levels were remarkably reduced. Additionally, in the diabetic model-NAC treatment group, collagen production and wound contraction were significantly increased. Systemic NAC administration accelerates wound healing in diabetes by reducing oxidative stress and inflammation and increasing collagen production. As a consequence, systemic NAC therapy can be effective in ameliorating wound healing in diabetes.

Anahtar Kelimeler

• wound healing
• N-acetylcysteine
• diabetes
• oxidative stress
• proinflammatory markers

Destekleyen Kurum

Giresun Üniversitesi BAP

Proje Numarası

FEN-BAP-A-150219-66

Teşekkür

The author thanks Giresun University Central Research Laboratory Application and Research Center (GRUMLAB) and the former director of the center Assoc. Prof. Dr. Murat YOLCU for their support.

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