Lifespan in Drosophila melanogaster Feeding with White Tea

Öz

White tea is a special tea made from the bud and young leaves of some varieties of Camellia sinensis L. Kuntze plant. In this study, it was aimed to determine the effects of white tea on larval mortality in Drosophila melanogaster and the lifespan. The effect of white tea on the lifespan was studied separately in female and male populations of D. melanogaster. An average of 100 individuals for each group was collected from non-mated male and female flies at the same age (1-3 days). Then, these individuals were fed for 2 hours in the flasks containing water extracts that are the control group with white tea at different concentrations (0,5; 1,0; 1,5 and 2,0 mL/100mL medium). As a result of our study, no decrease was observed in the larval mortality rates at any concentration we applied. This result has been interpreted as that plant extract does not have toxic effects in the experimental groups. In the results obtained from the second phase of the study, statistically, significant increases were observed in the lifespan parallel to the increase in concentration. This result was interpreted to have been related to the antioxidant content in white tea.

Anahtar Kelimeler

• White tea
• Drosophila melanogaster
• Lifespan
• Larval mortality

Beyaz Çay ile Beslenen Drosophila melanogaster’de Hayatta Kalma Oranı ve Yaşam Süresi

Öz

Beyaz çay, Camellia sinensis L. Kuntze bitkisinin bazı varyetelerinin tomurcuk ve genç yapraklarından yapılan özel bir çaydır. Bu çalışmada beyaz çayın Drosophila melanogaster’de larval mortalite ve ömür uzunluğu üzerine etkilerinin belirlenmesi amaçlanmıştır. Beyaz çayın ömür uzunluğu üzerine etkisi, D. melanogaster’ in dişi ve erkek populasyonlarında ayrı ayrı çalışılmıştır. Pupadan çıkan aynı yaştaki (1-3 günlük) çiftleşmemiş dişi ve erkek sineklerden, her bir grup için ortalama 100 birey toplanmıştır. Daha sonra, bu bireyler farklı konsantrasyonlarda (0.5; 1.0; 1.5 ve 2.0 mL/100mL besiyeri) beyaz çay ile kontrol grubu olan su ekstrelerini içeren şişelerde 2 saat beslenmişlerdir. Kontrol ve uygulama gruplarının tümünde, sayımlara ve uygulamaya en son birey ölene kadar devam edilmiştir. Çalışmamızın sonucunda, uyguladığımız hiçbir konsantrasyonda kontrol grubuna göre larval mortalite oranlarında herhangi bir azalış gözlenmemiştir. Çalışmanın ikinci aşamasından elde ettiğimiz sonuçlarda ise konsantrasyon artışına paralel olarak ömür uzunluğu oranlarında istatistiksel olarak anlamlı artışlar gözlenmiştir. Bu sonucun beyaz çaydaki antioksidan içeriği ile ilgili olabileceği düşünülmüştür.

Anahtar Kelimeler

• beyaz çay
• drosophila melanogaster
• ömür uzunluğu
• larval mortalite

Kaynakça

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