Determination of β-Sitosterol in Alchemilla caucasica Buser by GC-MS Method

Yıl 2018, Cilt: 11 Sayı: 2, 149 - 157, 31.08.2018

Esen Sezen Karaoğlan Bilal Yılmaz

https://doi.org/10.18185/erzifbed.412214

Öz

In this study, it was aimed to
develop a method for the determination of β-sitosterol by gas
chromatography-mass spectrometry method in Alchemilla
caucasica Buser. For β-sitosterol, selected ion monitoring and electron
ionization modes were used. Calibration linearity of the devoloped method was
obtained at 1-100 μg/mL concentration for β-sitosterol. The intra-day and
inter-day precision value for β-sitosterol was less than 4.99%. The accuracy of
the method was observed to be better than 4.60%. The detection and
quantification limits for β-sitosterol were found 0.05 and 0.15 μg/mL,
respectively. The amount of  β-sitosterol
in A. caucasica plant was found to be
22.6 μg/1.0 mg with percent recovery 2.26% for β-sitosterol. Also, this method
can be used for the determination of β-sitosterol in A. caucasica plant.

Anahtar Kelimeler

Alchemilla caucasica, β-sitosterol, GC-MS, Validation

Determination of β-Sitosterol in Alchemilla caucasica Buser by GC-MS Method

Öz

Bu çalışmada Alchemilla caucasica bitkisinde
β-sitosterol’ün gaz kromatografisi-kütle spektrometri yöntemiyle belirlenmesi
için bir yöntem geliştirmesi amaçlandı. β-sitosterol için, seçilmiş iyon
görüntüleme ve elektron iyonizasyon modları kullanıldı. Geliştirilen yöntemin
doğrusal aralığı β-sitosterol için 1-100 μg/mL olarak elde edildi. β-sitosterol
için gün-içi ve günler-arası kesinlik değerinin %4.99'dan daha düşüktü.
Yöntemin doğruluğunun %4.60'dan daha iyi olduğu görüldü. Gözlenebilme ve miktar
tayin sınırları β-sitosterol için sırasıyla 0.05 ve 0.15 μg/mL olarak bulundu. A. caucasica bitkisinde β-sitosterol
miktarı 2.26%’lık geri kazanımıyla 22.6 μg/1.0 mg olarak bulundu. Öyle ki bu
yöntem, A. caucasica bitkisinde
β-sitosterol’ün tayini için de kullanılabilir.

Anahtar Kelimeler

Alchemilla caucasica, β-sitosterol, GC-MS, Validation

Kaynakça

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