Yıl 2020, Cilt 13 , Sayı 1, Sayfalar 306 - 313 2020-03-20

Hasta Lepistes Balığından (Poecilia reticulata)İzole Edilen Aeromonas hydrophila’ nın Antibiyotik Duyarlılığı ve Histopatolojisi
Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)

Çiğdem ÜRKÜ [1]


Bu çalışma İstanbul ilinde bulunan bir akvaryum balığı üretim işletmesindeki hasta lepistes balıklarında görülen ölümlerin nedenini ortaya koymak ve izole edilen patojen bakterinin dokularda oluşturduğu hasarı ve antibiyotik duyarlılığını tespit etmek amacı ile yürütülmüştür. Eksternal olarak, ölmek üzere olan lepistes (Poecilia reticulata) balıklarında pullarda dökülme, vücut yüzeyinde ülseratif deri lezyonları ve abdominal genişleme görüldü; internal olarak karaciğerin renginde açılma, abdominal boşlukta sıvı birikimi tespit edildi. Karaciğer, böbrek ve dalak gibi iç organlardan Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA) besiyerlerine ekimler yapıldı. İnkubasyon sonrasında izole edilen tüm bakteriler fizyolojik, morfolojik ve biyokimyasal özellikleri ve API 20E profiline göre Aeromonas hydrophila olarak identifiye edildi. Bu izolatların (n=15) kloramfenikol ve florfenikol’e duyarlı olduğu tespit edildi. Histopatolojik olarak dalak, karaciğer, böbrek ve kalp dokusunda multifokal liquefaktif nekroz, tubuler böbrek epitelyumunda dejenerasyon ve nekroz, mide bezlerinde ve mukoza epitelyumunda dökülme, dalağın kırmızı pulpasında boşalma, bağırsağın mukoza epitelinde ve lamina propriasında dökülme yanı sıra nekrotik solungaç epitel hücrelerinde dökülme tespit edilmiştir.

Abstract

This study was carried out to determine the reason of mortality in guppy (Poecilia reticulata) kept in an aquarium fish-rearing farm in Istanbul province and confirm damage caused in tissues and antibiotic susceptibility of isolated pathogen bacteria. Externally diseased guppies (n=15) showed sloughing scales, ulcerative skin lesions on the body surface and abdominal distension; internally pale liver and accumulation of a liquid in the abdominal cavity were observed. Bacteriological inoculation from internal organs such as liver, spleen and kidney of guppies (n=15) were made onto Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA). After incubation, all isolated bacteria were identified as Aeromonas hydrophila according to their psychological, morphological, biochemical characteristic, and API 20E profile.  Also, these isolates (n=15) were determined to be sensitive to chloramphenicol and florfenicol. Histopathologically, multifocal liquefactive necrosis in the spleen, liver, kidney and heart, degeneration of tubular epithelium and necrosis in the kidney, desquamation of mucosa epithelium and necrotic gastric glands in the stomach, discharge of the white pulp in the spleen, desquamation of the lamina propria and mucosa epithelium in the intestine, and sloughed off the necrotic gill epithelium cells were observed.

 

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Orcid: 0000-0003-0381-9321
Yazar: Çiğdem ÜRKÜ (Sorumlu Yazar)
Kurum: İSTANBUL ÜNİVERSİTESİ, SU BİLİMLERİ FAKÜLTESİ
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 20 Mart 2020

Bibtex @araştırma makalesi { erzifbed544683, journal = {Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi}, issn = {1307-9085}, eissn = {2149-4584}, address = {}, publisher = {Erzincan Üniversitesi}, year = {2020}, volume = {13}, pages = {306 - 313}, doi = {10.18185/erzifbed.544683}, title = {Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)}, key = {cite}, author = {ÜRKÜ, Çiğdem} }
APA ÜRKÜ, Ç . (2020). Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi , 13 (1) , 306-313 . DOI: 10.18185/erzifbed.544683
MLA ÜRKÜ, Ç . "Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)". Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13 (2020 ): 306-313 <https://dergipark.org.tr/tr/pub/erzifbed/issue/53239/544683>
Chicago ÜRKÜ, Ç . "Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)". Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13 (2020 ): 306-313
RIS TY - JOUR T1 - Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata) AU - Çiğdem ÜRKÜ Y1 - 2020 PY - 2020 N1 - doi: 10.18185/erzifbed.544683 DO - 10.18185/erzifbed.544683 T2 - Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi JF - Journal JO - JOR SP - 306 EP - 313 VL - 13 IS - 1 SN - 1307-9085-2149-4584 M3 - doi: 10.18185/erzifbed.544683 UR - https://doi.org/10.18185/erzifbed.544683 Y2 - 2020 ER -
EndNote %0 Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata) %A Çiğdem ÜRKÜ %T Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata) %D 2020 %J Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi %P 1307-9085-2149-4584 %V 13 %N 1 %R doi: 10.18185/erzifbed.544683 %U 10.18185/erzifbed.544683
ISNAD ÜRKÜ, Çiğdem . "Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)". Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 13 / 1 (Mart 2020): 306-313 . https://doi.org/10.18185/erzifbed.544683
AMA ÜRKÜ Ç . Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020; 13(1): 306-313.
Vancouver ÜRKÜ Ç . Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata). Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020; 13(1): 313-306.