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Yıl 2013, Cilt: 6 Sayı: 2, 145 - 155, 18.03.2014

Kültiğin Çavuşoğlu , Saffet Sağır Kürşad Yapar

Öz

The aim of this study is to investigate the possible physiological and genotoxic effects of 1,4 Dioxane and the protective role of green tea extract to this effects in Swiss albino mice. Albino mice divided into one control group and five practice groups randomly. The body weights before and after practice period, erythrocyte and bone marrow quantity and frequency of chromosomal abnormalities of Albino mice are determined through a sensitive balance, using micronucleus test and by using erythrocyte micronucleus test (EMT) and chromosomal abnormalities test (CAT), respectively. It is determined that 1,4 Dioxane practice causes a statistically important reduction of body weight and increase in MN and frequency of chromosomal abnormalities according to control group. Green tea practice improves these negative effects of 1,4 Dioxane and causes an increase in body weight and a decrease in MN and frequency of chromosomal abnormalities though less than control group. As a result, green tea practice can be used in reduction of toxicity caused by chemicals as a toxicity limiter antioxidant in near future.

Anahtar Kelimeler

1 4 Dioxane albino mouse genotoxicity green tea

Kaynakça

  • Abe, S. and Sasaki, M. (1977). Chromosome Aberrations and Sister Chromatid Exchanges in Chinese Hamster Cells Exposed to Various Chemicals, Journal National Cancer Institute, 58, 6, 1635-1641.
  • ATSDR. (2006). Toxicological profile for 1,4-dioxane (update). U.S. Department of Health and Human Services. Agency for Toxic Substances and Disease Registry.
  • Benzio, I. F. F. and Szeto, Y. T. (1999). Total Antioxidant Capacity of Teas By The Ferric Reducing/Antioksidant Power Assay, Journal Agricultural and Food Chemical, 64, 633-636.
  • Beyersmann, D. and Hechtenberg, S. (1997). Cadmium, Gene Regulation and Cellular Signaling in Mammalian Cells, Toxicology and Applied Pharmacology, 144, 247–261.
  • Brusick, D. (1987). Principles of Genetic Toxicology, Plenum Pres, NY, London.
  • Drew, R.T. , Patel, J. M. and Lin, F. N. (1978). Changes in Serum Enzymes in Rats After Inhalation of Organic Solvents Singly and in Combination, Toxicology and Applied Pharmacology, 45, 809-819. Eman, A. M., Ola Hassan, E. H. (2005). Genotoxicity of 1,4 Dioxane in Mouse Bone Marrow: I. Effect on the Micronucleus Assay, Scientific Journal of King Faisal University, 6, 1426.
  • EPA. (2006). Treatment Technologies for 1,4- Dioxane. Fundamentals and Field Applications. EPA 542-R-06-009.
  • Fenench, M., Chang, W. P. , Kirsch-Volders, M. , Holland, N. , Bonassi, S. and Zeiger, E. (2003). Human Micronucleus Project. HUMN Project: Detailed Description of the Scoring Criteria for the CytokinesisBlock Micronucleus Assay Using Isolated Human Lymphocyte Cultures, Mutation Research, 534, 65–75.
  • Kociba, R. J. (1974). Chronic toxicity study of dioxane in the drinking water of Sherman rats. Midland, MI: Dow Chemical Company.
  • Mateuca, R. , Lombaert N. , Aka PV. , Decordier I. and Kirsch-Volders M. (2006). Chromosomal Changes: Induction, Detection Methods and Applicability in Human Biomonitoring, Biochemistry, 88, 11, 1515
  • Moeller, D.W. (1997). Environmental Health, Harvard University Press, Cambridge.
  • Mohr, T.K.G. (2001). Solvent Stabilizers, White Paper. Prepublication Copy. Santa Clara Valley Water District of California. San Jose, California. Morley, A. A. (1986). Cytokinesis-Blocked Micronucleus Method in Human Lymphocytes: Effect of In-Vivo Aging and Low Dose X-Irradiation, Mutation Research, 161, 193-198.
  • Murray, R. K. , Granner, D. K. , Mayes, P.A. & Rodwell, V.W. (1996). Harper’ın Biyokimyası. Barış Kitabevi. İstanbul.
  • Oguni, I. , Nasu, K. , Kanaya, S. , Ota, Y. , Yamamoto, S. and Nomura, T. (1989). Epidemiological and Experimental Studies on the Antitumor Activity by Green Tea Extract, Jpn J Nutr, 47, 93-102.
  • Okuda, T. , Mori, K. and Hayatsu, H. (1984). Inhibitory Effect of Tannins on Direct-Acting Mutation, Chem Pharm Bull, 32, 3755-3758.
  • Özkul, Y. , Dönmez, H. , Erenmemişoğlu, A. , Demirtaş, H. and İmamoğlu , N. (1997). Induction of Micronuclei by Smokeless Tobacco on Buccal Mucosa Cells of Habitual Users, Mutagenesis, 12, 285–287.
  • Pala, S. F. , Akkaya, F. , Tabakçıoğlu, K. , Tokatlı, F. , Uzal, C. , Parlar, Ş. ve Algüneş, Ç. (2008). Tüm Kromozom Taşıyan Mikronukleusların Biyolojik Doz Değerlendirmelerindeki Etkisi, Turkish Journal of Biology, 32, 283-290.
  • Rencüzoğulları, E. , İla, H. B. , Kayraldız, A. and Topaktaş, M. (2001)b. Chromosome Aberrations and Sister Chromatid Exchange in Cultured Human Lymphocytes Treated with Sodium Metabisulfite, a Food Preservative, Mutation Research, 490, 107-112.
  • Savage, J. R. (1976). Classification and Relationships of Induced Chromosomal Structural Changes, Journal of Medical Genetics, 13, 103–122.
  • Stefan, M. I. and Bolton, J. R. (1998). Mechanism of the Degradation of 1,4Dioxane in Dilute Aqueous Solution Using the UV/Hydrogen Peroxide Process, Environ Sci Technol, 32,1588–1595.
  • Stensvold, I., Tverdal, A. , Solvoll, K. and Foss, O.P. (1992). Tea Consumption. Relationship to Cholesterol, Blood Pressure and Coronary and Total Mortality, Preventive Medicine, 21, 546-53.
  • Sharma, A. K. (1972). Chromosome Techniques Theory and Practice, University Park Press, 575, Baltimore.
  • Te-Hsiu, M. A. , Zhou, X. , Loarco, G. F. , Arreola, G.G. and Lecona, S. (1995). Mouse–erythrocyte Micronucleus (MUS -EMN) Assay on the Clastogenicity of Industrial Wastewater, Revista Internacional de Contaminacion Ambiental, 11, 95–98.
  • Thiess, A. M, Tress, E. and Fleig I. (1976). Industrial-Medical Investigation Results in the Case of Workers Exposed to Dioxane(Ger.), Arbeitsmed Sozialmed Praventivmed, 11, 35-46.
  • Yang, Y. C. , Lu, F. H. , Wu, J. S. , Wu, C.H. and Chang, C.J. (2003). The Protective Effect of Habitual Tea Consumption on Hypertension, Archives of International Medicine, 164, 1534-40.
  • Yıldırım, A. ve Yıldırım, M. S. (2011). Matbaa Sanayinde Çalışan İşçilerin Bukkal Mukoza Hücrelerinde Mikronükleus ve Binükleotid Sıklığının Belirlenmesi, Tıp Araştırma Dergisi, 9, 1, 25-28. ***

1,4-DİOKSAN VERİLEN SWISS ALBİNO FARELERDE(♀) YEŞİL ÇAYIN BAZI FİZYOLOJİK VE GENOTOKSİK PARAMETRELER ÜZERİNE KORUYUCU ETKİSİ

Yıl 2013, Cilt: 6 Sayı: 2, 145 - 155, 18.03.2014

Kültiğin Çavuşoğlu , Saffet Sağır Kürşad Yapar

Öz

Bu çalışmada 1,4 Dioksanın Swiss albino farelerde muhtemel fizyolojik ve genotoksik etkileri ile yeşil çay özütünün bu etkilere karşı koruyucu rolü araştırılmıştır. Albino fareler rastgele bir kontrol, beş uygulama olmak üzere toplam altı gruba ayrılmışlardır. Fizyolojik parametrelerin indikatörü olarak vücut ağırlığı, genotoksik parametrelerin indikatörleri olarak ise mikronükleus (MN) ve kromozomal anormallik sıklıkları kullanılmıştır. 1,4 Dioksan uygulamasının kontrol grubuna göre vücut ağırlığında istatistiksel açıdan önemli bir azalmaya, MN ve kromozomal anormallik sıklığında ise bir artışa neden olduğu belirlenmiştir. Yeşil çay uygulaması ise 1,4 Dioksanın söz konusu olumsuz etkilerini iyileştirerek, kontrol grubu kadar olmasada vücut ağırlığında tekrar bir artışa, MN ve kromozomal anormallik sıklıklarında ise bir azalmaya neden olmuştur. Sonuç olarak yeşil çay uygulaması yakın gelecekte kimyasallar tarafından sebep olunan toksisitenin azaltılmasında “toksisite sınırlayıcı” bir antioksidan olarak kullanılabilir.

Anahtar Kelimeler

1 4 Dioksan albino fare genotoksisite yeşil çay

Kaynakça

  • Abe, S. and Sasaki, M. (1977). Chromosome Aberrations and Sister Chromatid Exchanges in Chinese Hamster Cells Exposed to Various Chemicals, Journal National Cancer Institute, 58, 6, 1635-1641.
  • ATSDR. (2006). Toxicological profile for 1,4-dioxane (update). U.S. Department of Health and Human Services. Agency for Toxic Substances and Disease Registry.
  • Benzio, I. F. F. and Szeto, Y. T. (1999). Total Antioxidant Capacity of Teas By The Ferric Reducing/Antioksidant Power Assay, Journal Agricultural and Food Chemical, 64, 633-636.
  • Beyersmann, D. and Hechtenberg, S. (1997). Cadmium, Gene Regulation and Cellular Signaling in Mammalian Cells, Toxicology and Applied Pharmacology, 144, 247–261.
  • Brusick, D. (1987). Principles of Genetic Toxicology, Plenum Pres, NY, London.
  • Drew, R.T. , Patel, J. M. and Lin, F. N. (1978). Changes in Serum Enzymes in Rats After Inhalation of Organic Solvents Singly and in Combination, Toxicology and Applied Pharmacology, 45, 809-819. Eman, A. M., Ola Hassan, E. H. (2005). Genotoxicity of 1,4 Dioxane in Mouse Bone Marrow: I. Effect on the Micronucleus Assay, Scientific Journal of King Faisal University, 6, 1426.
  • EPA. (2006). Treatment Technologies for 1,4- Dioxane. Fundamentals and Field Applications. EPA 542-R-06-009.
  • Fenench, M., Chang, W. P. , Kirsch-Volders, M. , Holland, N. , Bonassi, S. and Zeiger, E. (2003). Human Micronucleus Project. HUMN Project: Detailed Description of the Scoring Criteria for the CytokinesisBlock Micronucleus Assay Using Isolated Human Lymphocyte Cultures, Mutation Research, 534, 65–75.
  • Kociba, R. J. (1974). Chronic toxicity study of dioxane in the drinking water of Sherman rats. Midland, MI: Dow Chemical Company.
  • Mateuca, R. , Lombaert N. , Aka PV. , Decordier I. and Kirsch-Volders M. (2006). Chromosomal Changes: Induction, Detection Methods and Applicability in Human Biomonitoring, Biochemistry, 88, 11, 1515
  • Moeller, D.W. (1997). Environmental Health, Harvard University Press, Cambridge.
  • Mohr, T.K.G. (2001). Solvent Stabilizers, White Paper. Prepublication Copy. Santa Clara Valley Water District of California. San Jose, California. Morley, A. A. (1986). Cytokinesis-Blocked Micronucleus Method in Human Lymphocytes: Effect of In-Vivo Aging and Low Dose X-Irradiation, Mutation Research, 161, 193-198.
  • Murray, R. K. , Granner, D. K. , Mayes, P.A. & Rodwell, V.W. (1996). Harper’ın Biyokimyası. Barış Kitabevi. İstanbul.
  • Oguni, I. , Nasu, K. , Kanaya, S. , Ota, Y. , Yamamoto, S. and Nomura, T. (1989). Epidemiological and Experimental Studies on the Antitumor Activity by Green Tea Extract, Jpn J Nutr, 47, 93-102.
  • Okuda, T. , Mori, K. and Hayatsu, H. (1984). Inhibitory Effect of Tannins on Direct-Acting Mutation, Chem Pharm Bull, 32, 3755-3758.
  • Özkul, Y. , Dönmez, H. , Erenmemişoğlu, A. , Demirtaş, H. and İmamoğlu , N. (1997). Induction of Micronuclei by Smokeless Tobacco on Buccal Mucosa Cells of Habitual Users, Mutagenesis, 12, 285–287.
  • Pala, S. F. , Akkaya, F. , Tabakçıoğlu, K. , Tokatlı, F. , Uzal, C. , Parlar, Ş. ve Algüneş, Ç. (2008). Tüm Kromozom Taşıyan Mikronukleusların Biyolojik Doz Değerlendirmelerindeki Etkisi, Turkish Journal of Biology, 32, 283-290.
  • Rencüzoğulları, E. , İla, H. B. , Kayraldız, A. and Topaktaş, M. (2001)b. Chromosome Aberrations and Sister Chromatid Exchange in Cultured Human Lymphocytes Treated with Sodium Metabisulfite, a Food Preservative, Mutation Research, 490, 107-112.
  • Savage, J. R. (1976). Classification and Relationships of Induced Chromosomal Structural Changes, Journal of Medical Genetics, 13, 103–122.
  • Stefan, M. I. and Bolton, J. R. (1998). Mechanism of the Degradation of 1,4Dioxane in Dilute Aqueous Solution Using the UV/Hydrogen Peroxide Process, Environ Sci Technol, 32,1588–1595.
  • Stensvold, I., Tverdal, A. , Solvoll, K. and Foss, O.P. (1992). Tea Consumption. Relationship to Cholesterol, Blood Pressure and Coronary and Total Mortality, Preventive Medicine, 21, 546-53.
  • Sharma, A. K. (1972). Chromosome Techniques Theory and Practice, University Park Press, 575, Baltimore.
  • Te-Hsiu, M. A. , Zhou, X. , Loarco, G. F. , Arreola, G.G. and Lecona, S. (1995). Mouse–erythrocyte Micronucleus (MUS -EMN) Assay on the Clastogenicity of Industrial Wastewater, Revista Internacional de Contaminacion Ambiental, 11, 95–98.
  • Thiess, A. M, Tress, E. and Fleig I. (1976). Industrial-Medical Investigation Results in the Case of Workers Exposed to Dioxane(Ger.), Arbeitsmed Sozialmed Praventivmed, 11, 35-46.
  • Yang, Y. C. , Lu, F. H. , Wu, J. S. , Wu, C.H. and Chang, C.J. (2003). The Protective Effect of Habitual Tea Consumption on Hypertension, Archives of International Medicine, 164, 1534-40.
  • Yıldırım, A. ve Yıldırım, M. S. (2011). Matbaa Sanayinde Çalışan İşçilerin Bukkal Mukoza Hücrelerinde Mikronükleus ve Binükleotid Sıklığının Belirlenmesi, Tıp Araştırma Dergisi, 9, 1, 25-28. ***
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Kültiğin Çavuşoğlu

Saffet Sağır Bu kişi benim

Kürşad Yapar

Yayımlanma Tarihi 18 Mart 2014
Yayımlandığı Sayı Yıl 2013 Cilt: 6 Sayı: 2

Kaynak Göster

APA Çavuşoğlu, K., Sağır, S., & Yapar, K. (2014). 1,4-DİOKSAN VERİLEN SWISS ALBİNO FARELERDE(♀) YEŞİL ÇAYIN BAZI FİZYOLOJİK VE GENOTOKSİK PARAMETRELER ÜZERİNE KORUYUCU ETKİSİ. Erzincan University Journal of Science and Technology, 6(2), 145-155.