Investigating TIFY Genes for Salt Stress Adaptation in Quinoa (Chenopodium quinoa Willd.): A Genome-Wide Approach

Yıl 2025, Cilt: 18 Sayı: 1, 213 - 228, 28.03.2025

Esma Yigider

https://doi.org/10.18185/erzifbed.1581226

Öz

TIFY ailesi, TIFY, Jas ve GATA motiflerini içeren bitkilerin savunma mekanizmalarında ve stres faktörlerine karşı verdikleri yanıtta önemli rol oynayan bir gen ailesidir. TIFY gen ailesi birçok bitki türünde araştırılmış olmasına rağmen, kinoada henüz incelenmemiştir. Bu çalışmada, 16 Cq-TIFY geni tanımlanmış, bu genler Cq-TIFY-1’den Cq-TIFY-16’ya kadar numaralandırılarak yapısal ve işlevsel özellikleri karakterize edilmiştir. Tanımlanan Cq-TIFY proteinlerinin moleküler ağırlıkları 19,99 ile 48,59 kDa, amino asit sayıları 189 ile 450, teorik izoelektrik noktaları ise 4,84 ile 10,1 arasında değişmektedir. Filogenetik analiz sonuçlarına göre, TIFY genlerinin üç sınıfa ayrıldığı belirlenmiştir. Gen ailesinin farklı sınıflarındaki üyelerin gen yapılarının genellikle benzer olduğu belirlenmiştir. Kinoa’da yedi segmental duplikasyon geçirmiş gen tanımlanmış olup, Ka/Ks analizi bu genlerin evrimsel süreçte arındırıcı (negatif) seçilime maruz kaldığını göstermiştir. Chenopodium quinoa, Arabidopsis thaliana ve Spinacia oleracea türleri arasındaki TIFY genlerinin sinteni analizi, bu üç bitki arasında TIFY genleri açısından bir ilişki olduğunu ortaya koymuştur. Promotör analizi sonucunda, TIFY genlerinde strese duyarlı ve hormonla ilişkili cis-elementlerin varlığı ortaya çıkarılmıştır. Araştırmada, RNA-seq verileri, tuz stres koşulları altında kök ve sürgün dokularında Cq-TIFY genlerinin ifade modellerini incelemek için kullanılmıştır. Genlerin tuz stresi altındaki ifade profili köklerde ve sürgünlerde dokuya özgü olarak farklılık göstermiş ve ifadelerinde anlamlı bir artış belirlenmiştir. Bu sonuç, genlerin tuz toleransı mekanizmalarında rol oynayabileceğini düşündürmüştür. Bu çalışma, kinoadaki TIFY gen ailesine dair bilgimizi artırmakta ve klasik ıslah veya genetik mühendisliği yoluyla stres toleransını artırmaya yönelik bir temel oluşturmaktadır.

Anahtar Kelimeler

Abiotic stress, Cis-regulatory element, JAZ domain, Phylogenetic analysis

Etik Beyan

This study presents no ethical concerns related to its publication.

Investigating TIFY Genes for Salt Stress Adaptation in Quinoa (Chenopodium quinoa Willd.): A Genome-Wide Approach

Öz

Kaynakça

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