Vulpinic Acid Targets WNT/β-Catenin Signalling Pathway in HeLa Cells

Yıl 2025, Cilt: 18 Sayı: 2, 495 - 504, 31.08.2025

Şeyda Nur Kalin

https://doi.org/10.18185/erzifbed.1595902

Öz

Cervical cancer is one of the most common cancers in women. Due to the side effects and inadequate treatment methods of current cancer drugs used in the treatment of cervical cancer, it is important to develop new treatment strategies. Vulpinic acid (VA), a natural lichen secondary metabolite with many remarkable biological activities, has no detailed study describing its potential anti-cancer molecular mechanism in cervical cancer HeLa cell line. We reported in our previous study that VA exhibited anti-proliferative, apoptotic, and anti-migratory properties in HeLa cells and the IC50 dose of VA in HeLa cells was calculated as 66.53 µg/mL at 48 h. The effect of VA on the WNT/β-catenin signaling pathway, which plays a role in various biological processes including tumorigenesis, cell proliferation, cell cycle regulation, embryogenesis, metastasis, cellular differentiation, apoptosis and drug resistance, is unknown. In this study, we aimed to elucidate whether VA exerts its antimigratory effect on HeLa cells treated with IC50 dose through the WNT/β-catenin signalling pathway. In summary, this study demonstrated that the suppression of migration of HeLa cells by VA may be mediated by inhibition of the WNT/β-catenin signalling pathway. VA may be a natural active compound candidate for the therapy of human cervical cancer and may be among the inhibitory candidates of the WNT/β-catenin signalling pathway.

Anahtar Kelimeler

lichen secondary metabolite , antimigratory effect , gene expression

Proje Numarası

No funding was received to assist with the preparation of this manuscript.

Vulpinik Asit HeLa Hücrelerinde WNT/β-Catenin Sinyal Yolağını Hedefler

Öz

Rahim ağzı kanseri kadınlarda en sık görülen kanserlerden biridir. Rahim ağzı kanseri tedavisinde kullanılan mevcut kanser ilaçlarının yan etkileri ve yetersiz tedavi yöntemleri nedeniyle yeni tedavi stratejilerinin geliştirilmesi önem arz etmektedir. Birçok dikkate değer biyolojik aktiviteye sahip doğal bir liken sekonder metaboliti olan vulpinik asit (VA), serviks kanseri HeLa hücre hattında potansiyel anti-kanser moleküler mekanizmasını açıklayan ayrıntılı bir çalışmaya sahip değildir. Önceki çalışmamızda VA'nın HeLa hücrelerinde anti-proliferatif, apoptotik ve anti-göç özellikleri sergilediğini ve VA'nın HeLa hücrelerindeki IC50 dozunun 48 saatte 66,53 µg/mL olarak hesaplandığını bildirmiştik. VA'nın tümörigenez, hücre proliferasyonu, hücre döngüsü düzenlemesi, embriyogenez, metastaz, hücresel farklılaşma, apoptoz ve ilaç direnci gibi çeşitli biyolojik süreçlerde rol oynayan WNT/β-katenin sinyal yolağı üzerindeki etkisi bilinmemektedir. Bu çalışmada, VA'nın IC50 dozu ile tedavi edilen HeLa hücreleri üzerindeki antimigratör etkisini WNT/β-katenin sinyal yolağı üzerinden gösterip göstermediğini aydınlatmayı amaçladık. Özetle, bu çalışma HeLa hücrelerinin göçünün VA tarafından baskılanmasına WNT/β-katenin sinyal yolağının inhibisyonunun aracılık edebileceğini göstermiştir. VA, insan serviks kanserinin tedavisi için doğal bir aktif bileşik adayı olabilir ve WNT/β-katenin sinyal yolağının inhibitör adayları arasında yer alabilir.

Anahtar Kelimeler

liken sekonder metabolit , anti-migratif etki , gen ekspresyonu

Proje Numarası

No funding was received to assist with the preparation of this manuscript.

Kaynakça

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