DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS

Kaan Hürkan; Merve Ballı Yüksel; Yasemin Kemeç Hürkan; Neslihan Demir

doi:10.18036/estubtdc.598852

EN TR

DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS

Abstract

ABSTRACT

In this study we evaluated the secondary metabolites, total phenolic (TPC) and flavonoid contents (TFC), antioxidant and antimicrobial activities of salep orchids, Anacamptis morio, Anacamptis pyramidalis, Neotinea tridentata, Ophrys mammosa, Ophrys lutea, and Ophrys speculum. DPPH free radical scavenging assay was used to determine the antioxidant activities of n-hexane, chloroform, methanol and water extracts of the plants. The antimicrobial activities were also determined by the Broth micro-dilution method. The extracts were studied for antimicrobial activity by the Minimum Inhibitory Concentration (MIC) approach against seven clinical pathogenic bacteria and two fungi. Phytochemical screening revealed that the presences of coumarins, flavonoids, flavanones, cardiac glycosides, proteins and quinones. The extracts had variable TPC and TFC, with values of 4.46 ± 0.19–45.83 ± 1.86 mg gallic acid equivalent/g dry weight and 0.67 ± 0.04–8.64 ± 0.37 mg quercetin equivalent/g dry weight respectively. O. speculum had the highest (35.12%) antioxidant activity, followed by O. mammosa (33.17%). Chloroform extracts of all species showed significant antioxidant and antimicrobial activity. These bioactivities of the chloroform extracts were positively associated with the total phenolic and flavonoid contents. The MIC concentrations ranged from 0.156–20 mg/mL. The present investigation shows that the extracts of these species, especially chloroform extracts, could be used as potential antioxidant and antimicrobial sources.

Keywords

Salep orchids,Antioxidant activity,DPPH• assay,Antimicrobial activity,Minimum inhibitory concentration

BAZI ÖNEMLİ SALEP ORKİDESİ TÜRLERİNİN TOPLAM FENOLİK VE FLAVONOİT İÇERİKLERİNİN, ANTİOKSİDAN VE ANTİMİKROBİYAL AKTİVİTELERİNİN BELİRLENMESİ

Abstract

Bu çalışmada; Anacamptis morio, Anacamptis pyramidalis, Neotinea tridentata, Ophrys mammosa, Ophrys lutea ve Ophrys speculum salep orkidelerinin sekonder metabolitleri, toplam fenolik (TPC) ve flavonoit bileşikleri (TFC), antioksidan ve antimikrobiyal aktiviteleri ölçülmüştür. DPPH serbest radikal temizleme yöntemi ile bitkilerin n-hekzan, kloroform, methanol ve su özütlerinde antioksidan aktiviteler belirlenmiştir. Antimikrobiyal aktiviteler Broth mikrodilüsyon yöntemi ile belirlenmiştir. Özütler yedi klinik patojen bakteriye ve iki fungusa karşı taranmıştır. Fitokimyasal taramada kumarinler, flavanoitler, flavanonlar, kardiyak glikozitler, proteinler ve kinonlar bulunduğunu ortaya çıkarılmıştır. Özütlerin değişken TPC ve TFC değerlerine sahip olduğu gözlenmiştir (TPC 4.46 ± 0.19–45.83 ± 1.86 mg gallik asit eşdeğer/g kuru ağırlık ve TFC 0.67 ± 0.04–8.64 ± 0.37 mg kuersetin eşdeğer/g kuru ağırlık). Çalışmada O. speculum türünün %35.12 ile en yüksek antioksidan aktiviteye sahip olduğu ve onu %33.17 ile O. mammosa türünün takip ettiği belirlenmiştir. Bütün türlerde kloroform özütlerinin en yüksek antioksidan ve antimikrobiyal aktiviteye sahip olduğu görülmüştür. Kloroform özütlerinin bu biyoaktiviteleri toplam fenolik ve flavanoit bileşikleri ile pozitif olarak ilişkilidir. MİK konsantrasyonları 0.156-20 mg/mL oranındadır. Bu çalışma, kullanılan salep orkidelerinin özellikle kloroform özütlerinin, potansiyel antioksidan ve antimikrobiyal kaynakları olabileceğini göstermektedir.

Keywords

Salep orkideleri,Antioksidan aktivite,DPPH• analizi,Antimikrobiyal aktivite,Minimum inhibisyon konsantrasyonu

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Kaan Hürkan ^*
0000-0001-5330-7442
Türkiye

Merve Ballı Yüksel This is me
0000-0001-7205-5885
Türkiye

Yasemin Kemeç Hürkan
0000-0003-4089-2683
Türkiye

Neslihan Demir
0000-0002-2347-8344
Türkiye

Publication Date

July 31, 2019

Submission Date

October 3, 2018

Acceptance Date

January 29, 2019

Published in Issue

Year 2019 Volume: 8 Number: 2

DOI

https://doi.org/10.18036/estubtdc.598852

IZ

https://izlik.org/JA99SP24WW

APA

Hürkan, K., Ballı Yüksel, M., Kemeç Hürkan, Y., & Demir, N. (2019). DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 8(2), 191-202. https://doi.org/10.18036/estubtdc.598852

AMA

1.Hürkan K, Ballı Yüksel M, Kemeç Hürkan Y, Demir N. DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2019;8(2):191-202. doi:10.18036/estubtdc.598852

Chicago

Hürkan, Kaan, Merve Ballı Yüksel, Yasemin Kemeç Hürkan, and Neslihan Demir. 2019. “DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8 (2): 191-202. https://doi.org/10.18036/estubtdc.598852.

EndNote

Hürkan K, Ballı Yüksel M, Kemeç Hürkan Y, Demir N (July 1, 2019) DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8 2 191–202.

IEEE

[1]K. Hürkan, M. Ballı Yüksel, Y. Kemeç Hürkan, and N. Demir, “DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS”, Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 8, no. 2, pp. 191–202, July 2019, doi: 10.18036/estubtdc.598852.

ISNAD

Hürkan, Kaan - Ballı Yüksel, Merve - Kemeç Hürkan, Yasemin - Demir, Neslihan. “DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS”. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8/2 (July 1, 2019): 191-202. https://doi.org/10.18036/estubtdc.598852.

JAMA

1.Hürkan K, Ballı Yüksel M, Kemeç Hürkan Y, Demir N. DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2019;8:191–202.

MLA

Hürkan, Kaan, et al. “DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 8, no. 2, July 2019, pp. 191-02, doi:10.18036/estubtdc.598852.

Vancouver

1.Kaan Hürkan, Merve Ballı Yüksel, Yasemin Kemeç Hürkan, Neslihan Demir. DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2019 Jul. 1;8(2):191-202. doi:10.18036/estubtdc.598852