DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS

Yıl 2019, Cilt: 8 Sayı: 2, 191 - 202, 31.07.2019

Kaan Hürkan , Merve Ballı Yüksel Yasemin Kemeç Hürkan , Neslihan Demir

https://doi.org/10.18036/estubtdc.598852

Cited By: 7

Öz

ABSTRACT

 In
this study we evaluated the secondary metabolites, total phenolic (TPC) and
flavonoid contents (TFC), antioxidant and antimicrobial activities of salep
orchids, Anacamptis morio, Anacamptis pyramidalis, Neotinea tridentata, Ophrys mammosa, Ophrys lutea, and Ophrys
speculum. DPPH free radical scavenging assay was used to determine the
antioxidant activities of n-hexane,
chloroform, methanol and water extracts of the plants. The antimicrobial
activities were also determined by the Broth micro-dilution method. The
extracts were studied for antimicrobial activity by the Minimum Inhibitory
Concentration (MIC) approach against seven clinical pathogenic bacteria and two
fungi. Phytochemical screening revealed that the presences of coumarins,
flavonoids, flavanones, cardiac glycosides, proteins and quinones. The extracts
had variable TPC and TFC, with values of 4.46 ± 0.19–45.83 ± 1.86 mg gallic
acid equivalent/g dry weight and 0.67 ± 0.04–8.64 ± 0.37 mg quercetin
equivalent/g dry weight respectively. O.
speculum had the highest (35.12%) antioxidant activity, followed by O. mammosa (33.17%). Chloroform extracts
of all species showed significant antioxidant and antimicrobial activity. These
bioactivities of the chloroform extracts were positively associated with the
total phenolic and flavonoid contents. The MIC concentrations ranged from
0.156–20 mg/mL. The present investigation shows that the extracts of these
species, especially chloroform extracts, could be used as potential antioxidant
and antimicrobial sources.

 

Anahtar Kelimeler

Salep orchids, Antioxidant activity, DPPH• assay, Antimicrobial activity, Minimum inhibitory concentration

BAZI ÖNEMLİ SALEP ORKİDESİ TÜRLERİNİN TOPLAM FENOLİK VE FLAVONOİT İÇERİKLERİNİN, ANTİOKSİDAN VE ANTİMİKROBİYAL AKTİVİTELERİNİN BELİRLENMESİ

Öz

Bu çalışmada; Anacamptis morio,
Anacamptis pyramidalis, Neotinea tridentata, Ophrys mammosa, Ophrys lutea ve Ophrys
speculum salep orkidelerinin
sekonder metabolitleri, toplam fenolik (TPC) ve flavonoit bileşikleri (TFC),
antioksidan ve antimikrobiyal aktiviteleri ölçülmüştür. DPPH serbest radikal
temizleme yöntemi ile bitkilerin n-hekzan,
kloroform, methanol ve su özütlerinde antioksidan aktiviteler belirlenmiştir.
Antimikrobiyal aktiviteler Broth mikrodilüsyon yöntemi ile belirlenmiştir.
Özütler yedi klinik patojen bakteriye ve iki fungusa karşı taranmıştır.
Fitokimyasal taramada kumarinler, flavanoitler, flavanonlar, kardiyak
glikozitler, proteinler ve kinonlar bulunduğunu ortaya çıkarılmıştır. Özütlerin
değişken TPC ve TFC değerlerine sahip olduğu gözlenmiştir (TPC 4.46 ±
0.19–45.83 ± 1.86 mg gallik asit eşdeğer/g kuru ağırlık ve TFC 0.67 ± 0.04–8.64
± 0.37 mg kuersetin eşdeğer/g kuru ağırlık). Çalışmada O. speculum türünün %35.12 ile en yüksek antioksidan aktiviteye sahip
olduğu ve onu %33.17 ile O. mammosa türünün
takip ettiği belirlenmiştir. Bütün türlerde kloroform özütlerinin en yüksek antioksidan
ve antimikrobiyal aktiviteye sahip olduğu görülmüştür. Kloroform özütlerinin bu
biyoaktiviteleri toplam fenolik ve flavanoit
bileşikleri ile pozitif olarak ilişkilidir. MİK konsantrasyonları 0.156-20
mg/mL oranındadır. Bu çalışma, kullanılan salep orkidelerinin özellikle
kloroform özütlerinin, potansiyel antioksidan ve antimikrobiyal kaynakları
olabileceğini göstermektedir.

 

Anahtar Kelimeler

Salep orkideleri, Antioksidan aktivite, DPPH• analizi, Antimikrobiyal aktivite, Minimum inhibisyon konsantrasyonu

Kaynakça

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