CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH

Yıl 2020, Cilt: 9 Sayı: 1, 98 - 108, 31.01.2020

Ömer Yamaner , Bengi Erdağ

https://doi.org/10.18036/estubtdc.569370

Öz

In
this study, A standart protocol to callus formation and adventitious shoot regeneration of Hypericum adenotrichum Spach via direct or indirect organogenesis
has been described.

Callus
induction was carried out by using leaves which were collected from their
native environment. The maximum callus induction frequency has been observed on
MS media containing 4 mgL^-1 BA and 0.2 mgL^-1 NAA. These
calli were not induced to shoot regeneration. Shoot formation was obtained by
transferring the calluses from BA-containing media to MS medium containing 0.5
mgL^-1 KIN. Maximum shoot number was observedin transferred calluses from
callus induction medium containing 3 mgL^-1 BA to media containing
0.5 mgL^-1 KIN.

Direct
shoot formation developed on leaf explants of H. adenotrichum on MS
media containing KIN alone. The highest direct shoot development was observed on
MS medium with 1 mgL^-1 KIN.

 

Anahtar Kelimeler

Hypericum adenotrichum, callus, , endemic plant, , adventitious shoot

Destekleyen Kurum

Aydın Adnan Menderes Üniversitesi

Proje Numarası

FBE- 09013

Teşekkür

This study was part of the pH D thesis of Ömer Yamaner and financially supported by Aydın Adnan Menderes University Scientific Research Project Department (Project no: FBE- 09013). Abstract part of the study was presented as poster presentation at the 21st National Biology Congress, 3-7 September 2012, İzmir TURKEY.

Hypericum adenotrichum SPACH‘UN KALLUS İNDÜKSİYONU VE ADVENTİF SÜRGÜN REJENERASYONU

Öz

Bu
çalışmada, Hypericum adenotrichum
Spach ‘un kallus indüksiyonu ve direkt veya dolaylı organogenez yolu ile
adventif sürgün rejenerasyonu için standart bir protokol tanımlanmıştır.

Doğadan
toplanan bitkilerin yaprak eksplantlarından kallus oluşumu gözlenmiştir.
Maksimum kallus indüksiyon frekansı 4 mgL^-1 BA ve 0.2 mgL^-1
NAA ile kombine edilmiş MS ortamında elde edilmiştir. Fakat bu kalluslardan
sürgün rejenerasyonu indüklenememiştir. BA’nın tek başına kullanıldığı MS besi
ortamlarından elde edilen kalluslar, 0.5 mgL^-1 KIN içeren MS besi
ortamında alt kültüre alındıklarında sürgün oluşumu gerçekleşmiştir. Maksimum
sürgün sayısı 3 mgL^-1 BA içeren kallus indüksiyon ortamlarından
alınan kallusların 0.5 mgL^-1 KIN içeren ortamlara aktarılması ile
elde edilmiştir. Direkt sürgün oluşumu ise Kinetinin tek başına kullanıldığı MS
besi ortamlarında gerçekleşmiştir. Maksimum sürgün sayısı 1 mgL^-1
KIN içeren MS besi ortamında gözlenmiştir.

Anahtar Kelimeler

Hypericum adenotrichum, kallus, endemik plant, adventif sürgün

Proje Numarası

FBE- 09013

Kaynakça

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