BIOCHAR-SUPPORTED IN VITRO CULTURES OF Lavandula officinalis L.

Yıl 2024, Cilt: 13 Sayı: 2, 133 - 142, 30.07.2024

Pınar Nartop , Sena Ozdıl Şener , Seray Begüm Gök

https://doi.org/10.18036/estubtdc.1404374

Öz

Plants are the sources of valuable biomass that are being currently used in many areas. It is important to produce high biomass for efficient commercial production. Amongst the many factors that affect in vitro propagation of plants, changing or enriching the media composition is one of the commonly used techniques in micropropagation of plants. Biochar is a solid product obtained from organic wastes and because of its rich composition, it has many beneficial effects on plants. In our study, Lavandula officinalis plantlets were subjected to two types of biochars (Geocharged biochar and Biorfe biochar) at 0.5 and 2 g/L concentrations and their effects were investigated by means of plant growth, biomass accumulation and biochemical composition. The results showed that 0.5 g/L concentration of biochar had better effects than 2 g/L concentration and except for biochemical composition, biochar type had no significant effect on plant growth and biomass accumulation. Mean root dry weights and multiple shoot formations/explant enhanced up to 3.7 and 4.17 times higher than the control at 0.5 g/L concentration. Explant browning was also detected lower in biochar-applied media. The differences between biochemical accumulations of different media were also found statistically significant. The total concentrations of phenolics and flavonoids and radical scavenging activities were detected lower when biochars were applied. The total antioxidant concentration was higher in the control group. These findings showed that biochars lowered the negative effects of the culture conditions for L. officinalis plantlets.

Anahtar Kelimeler

Lavandula officinalis, Micropropagation, Biochar, Biomass and biochemical accumulations

Etik Beyan

----

Destekleyen Kurum

----

Proje Numarası

---

Teşekkür

----

Kaynakça

• [1] Mathur J, Koncz C. Callus Culture and Regeneration. In: Martínez-Zapater JM, Salinas J, editors. Methods in Molecular BiologyTM: Arabidopsis Protocols, New Jersey: Humana Press, 1998. 82, pp. 31-35p.
• [2] Chawla HS. Introduction to Plant Biotechnology. Second edition, New Hampshire, United States of America, USA: Science Publishers Inc., 2002.
• [3] Nartop P. Engineering of Biomass Accumulation and Secondary Metabolite Production in Plant Cell and Tissue Cultures. In. Ahmad P, Ahanger MA, Singh VP, editors. Plant Metabolites and Regulation Under Environmental Stress, Chapter 9, Elsevier, 2018, pp. 169-194, ISBN: 978-0-12-812689-9.
• [4] Rawat J, Saxena J, Sanwal P. Biochar: A Sustainable Approach for Improving Plant Growth and Soil Properties, Biochar - An Imperative Amendment for Soil and the Environment, 2019. DOI: http://dx.doi.org/10.5772/intechopen.82151.
• [5] Bonanomi G, Ippolito F, Scala FA. Black Future For Plant Pathology? Biochar As A New Soil Amendment For Controlling Plant Diseases. J Plant Pathol. 2015; 97(2): 223-234.
• [6] Jha P, Biswas AK, Rao AS. Biochar in agriculture – Prospects and related implications. Curr Sci 2010; 99(9): 1218-1225.
• [7] Lehmann J, Gaunt J, Rondon M. Bio-char sequestration in terrestrial ecosystems – A review. Mitig Adapt Strateg Glob Chang 2006; 11: 403-427. [8] Chang Y, Rossi L, Zotarelli L, Gao B, Shahid MA, Sarkhosh A. Biochar improves soil physical characteristics and strengthens root architecture in Muscadine grape (Vitis rotundifolia L.). Chem Biol Technol Agric 2021; 8:7.
• [9] Liang JF, Li QW, Gao JQ, Feng JG, Zhang XY, Wu YQ, Yu FH. Biochar rhizosphere addition promoted Phragmites australis growth and changed soil properties in the Yellow River Delta. Sci Total Environ 2021; 761: 143291.
• [10] Guo LL, Borno ML, Niu WQ, Liu FL. Biochar amendment improves shoot biomass of tomato seedlings and sustains water relations and leaf gas exchange rates under different irrigation and nitrogen regimes. Agric Water Manag 2021; 106580.
• [11] Farhangi-Abriz S, Torabian S. Biochar Increased Plant Growth-Promoting Hormones and Helped to Alleviates Salt Stress in Common Bean Seedlings. J Plant Growth Regul 2015; 37: 591–601.
• [12] Giannoulisa KD, Evangelopoulos V, Gougoulias N, Wogiatzi E. Lavender organic cultivation yield and essential oil can be improved by using bio-stimulants. Acta Agric Scand B Soil Plant Sci 2020; 70(8): 648–656.
• [13] Miclea I, Suhani A, Zahan M, Bunea A. Effect of Jasmonic Acid and Salicylic Acid on Growth and Biochemical Composition of In-Vitro-Propagated Lavandula angustifolia Mill Agron 2020; 10: 1722.
• [14] Lloyd GB, McCown BH. Commercial-feasible micropropagation of mountain laurel-Kalmia latifolia by use of shoot-tip culture. Proc Int Plant Prop Soc 1980; 30: 421–427.
• [15] Stoica R, Velea S, Ilie L, Calugareanu M, Ghimis BS, Ion R-M. The infuence of ethanol concentration on the total phenolics and antioxidant activity of Scenedesmus opoliensis algal biomass extracts. Rev Chim (Bucharest) 2013; 64(3): 304–306.
• [16] Desta ZY, Cherie DA. Determination of antioxidant and antimicrobial activities of the extracts of aerial parts of Portulaca quadrifda. Chem Cent J 2018; 12:146
• [17] İçli N. Total antioxidant capacity, total phenolic compounds and total favonoid amounts of darak dalı and basil which are materials of tarhana soup of Kastamonu. Sağlık Akademisi Kastamonu. 2018; 3(3): 171–184.
• [18] Ahmed D, Fatima K, Saeed R. Analysis of phenolic and flavonoid contents, and the anti-oxidative potential and lipid peroxidation inhibitory activity of methanolic extract of Carissa opaca roots and its fractions in diferent solvents. Antioxidants 2014; 3: 671–683.
• [19] Di Lonardo, S., Vaccari, F.P., Baronti, S., Capuana M., Bacci L., Sabatini F., Lambardi M., Miglietta F. Biochar successfully replaces activated charcoal for in vitro culture of two white poplar clones reducing ethylene concentration. Plant Growth Regul 2013; 69: 43–50.
• [20] Lehmann J, Rillig MC, Thies J, Masiello CA, Hockaday WC, Crowley D. Biochar effects on soil biota – A review. Soil Biol Biochem 2011; 43(9): 1812-1836.
• [21] Hammer E.C., Forstreuter M., Rilliga M.C., Kohlera J. Biochar increases arbuscular mycorrhizal plant growth enhancement and ameliorates salinity stress. Appl Soil Ecol 2015; 96: 114-121.
• [22] Hashemi SB, Momayezi M, Talee D. Biochar Effect on Cadmium Accumulation and Phytoremediation Factors by Lavender (Lavandula stoechas L.). Open J Ecol 2017; 7:447-459.
• [23] Mohamed-Yasseen, Y. Application of charcoal in horticulture. Trop Fruit News 1994; 28:7.
• [24] Bennett RC, Wallsgrove RM. Secondary metabolites in plant defencemechanisms. New Phytol 1994; 127: 617-633.
• [25] Scott CD, Dougall DK. Plant cell tissue culture - A potential source of chemicals. Oak Ridge National Laboratory, Department of Botany, Tenneesse, USA; 1987; pp 1-34.
• [26] Kul R, Ekinci M, Turan M, Yıldırım E. Impact of biochar on growth, physiology and antioxidant activity of common bean subjected to salinity stress. Glob J Bot Sci 2021; 9: 8-13.