Sıçanlarda Trikloroasetik Asit ile İndüklenen Deneysel İntrauterin Adezyonlarda Kök Hücre Tedavisi

Öz

Kemik iliğinden elde edilen kök hücrelerin, sıçanlarda kimyasal kullanılarak oluşturulan intrauterin adezyon modellemesinde rahim içi yapışıklıkların giderilmesinde ve blastosistin endometriuma implantasyonunun yeniden sağlanmasında etkinliği ve rolü araştırıldı. Deney modeli, trikloroasetik asit kullanılarak tek uterin hornda meydana gelen hasara karşı, yalnızca kültür besiyeri (CM), kök hücre ve 48 saatlik Niş kullanılarak farklı grup ve iki alt grup oluşturulmuştur. Çalışmada toplam 30 dişi ve 3 erkek rat kullanıldı. Akut fazda model oluşumundan hemen sonra tedaviye başlandı ve 10 gün sonra denekler gebe kalmaları için erkek sıçanlarla aynı kafese yerleştirildi. Daha sonra gebelik durumu ve doğan yavru sayısı değerlendirildi. Histolojik değerlendirme hematoksilen-eozin boyaması ile yapıldı. Yenidoğan sayısına göre hem histolojik hem de morfolojik değerlendirmelerde kök hücre uygulanan gruplarda diğer gruplara göre istatistiksel olarak anlamlı fark (p<0.05) bulundu. Akut dönemde bölgesel kök hücre uygulamasının rahim içi yapışıklıkları ortadan kaldırabileceği ve endometriyumu implantasyona daha uygun hale getirebileceği belirlendi. Sonuç olarak tedavi gruplarında endometrial kalınlık, bez sayısı ve damarlanmanın artması, fibröz alanların azalması ve adeziv alanların gerilemesi sonucu yenidoğan sayısındaki artış deneysel intrauterin çalışmaların kliniğe uyarlanması açısından umut verici olmuştur.

Anahtar Kelimeler

• Kök hücre
• İntrauterin adezyon
• rat
• histoloji

Stem Cell Therapy in Rats with Experimental Intrauterine Adhesions Induced by Trichloroacetic Acid

Abstract

The effectiveness and role of stem cells obtained from bone marrow in removing the adhesion formed in intrauterine adhesion modeling using chemicals in rats and restoring the implantation of the blastocyst to the endometrium were investigated. The experimental model was created in a single horn using trichloroacetic acid. Three different groups and two subgroups were formed as only culture medium (CM), stem cell and 48-hour medium (Niche). A total of 30 female and 3 male rats were used in the study. Treatment was started immediately after model formation in the acute phase, and 10 days later, the subjects were placed in the same cage with male rats for conception. Then, the pregnancy status and the number of puppies born were evaluated. Histological evaluation was performed with hematoxylin-eosin staining.A statistically significant difference (p<0.05) was found in the stem cell applied groups compared to the other groups in both histological and morphological evaluations according to the number of newborns. It has been determined that regional stem cell application in the acute period can remove adhesions in uterine adhesions and make the endometrium more suitable for implantation. As a result, the increase in the number of newborns as a result of the increase in endometrial thickness, number of glands and vascularization, decrease in fibrous areas and regression of adhesive areas in the treatment groups is promising in terms of carrying experimental intrauterine studies to the clinic.

Keywords

• Stem cell
• intrauterine adhesion
• rat
• histology

Kaynakça

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