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This study aims to explore the role of lesson analysis in the development of mathematical knowledge for teaching. For this purpose, a graduate course based on lesson analysis was designed for novice mathematics teachers. Throughout the course the teachers watched videos of group-mates and discussed the issues they identified in terms of student-teacher relationship for a student centered instruction. Analysis over the video made teachers notice points and came to realize the thoughts of students; they had otherwise missed at the classroom. They achieved improvements regarding the awareness of the need to identify the cases presenting the most difficulty to the students in terms of teaching, and the necessity to take precautions regarding such points, to learn about the reason of the difficulty, and to pay attention to the statements by the students. Therefore, it is possible to note that the teachers improved themselves in terms of student’ knowledge with respect to the mathematical knowledge for teaching. mathematics teacher, lesson analysis, mathematical knowledge for teaching.