In Situ Synthesis of Catecholamines-Inorganic Nanoflowers and Enhanced Antimicrobial Activity

Yıl 2025, Cilt: 34 Sayı: 3, 488 - 498, 30.12.2025

Çağla Çelik Yoldaş , Nilay Ildiz , Sadık Küçükgünay , İsmail Öçsoy

https://doi.org/10.34108/eujhs.1767146

Öz

Hybrid nanoflowers synthesized from proteins, enzymes, amino acids and various plant extracts have attracted considerable interest and are used for a variety of purposes. Notably, they exhibit increased stability, as well as enhanced catalytic, antimicrobial and antioxidant activity compared to free molecules. Most synthesis techniques are expected to occur in a solution medium. In this study, we synthesized catecholamine hybrid nanoflowers in situ as a result of a one-day incubation. The in situ catecholamine nanoflower that we synthesized on the surface of a glass exhibits high antimicrobial activity. Thanks to this feature, they have the potential to be used as antimicrobial surface coatings. For surface-bound synthesis, we selected a glass surface as the model surface and catecholamines (dopamine, epinephrine and norepinephrine) as the model organic components. We characterized the in situ catecholamine nanoflower using Scanning Electron Microscopy and contact angle analysis. Additionally, we tested the antimicrobial activities of the in situ catecholamine nanoflower coating against the bacteria Staphylococcus aureus and Escherichia coli and the fungus Candida albicans. The results revealed that the antimicrobial activity of the in situ catecholamine nanoflower coatings had increased significantly. In view of the findings, it is expected that they will have a successful impact as part of antimicrobial surface coatings.

Anahtar Kelimeler

Antibacterial coating , catecholamines , nanoflower

Etik Beyan

This study does not require ethical committee approval. Clinical samples were not used.

Teşekkür

This research did not receive support from any funding agency/industry.

Katekolamin-İnorganik Nano Çiçeklerin Yerinde Sentezi ve Gelişmiş Antimikrobiyal Aktiviteleri

Öz

Proteinler, enzimler, amino asitler ve çeşitli bitki özlerinden sentezlenen hibrit nano çiçekler büyük ilgi görmüş ve çeşitli amaçlarla kullanılmaktadır. Özellikle, serbest moleküllerle karşılaştırıldığında daha yüksek stabiliteye sahip olmanın yanı sıra, katalitik, antimikrobiyal ve antioksidan aktiviteleri de daha yüksektir. Sentez tekniklerinin çoğu bir çözelti ortamında gerçekleştirilir. Bu çalışmada ise, bir günlük inkübasyon sonucunda katekolamin hibrit nano çiçekleri in situ olarak sentezledik. Cam yüzeyinde sentezlediğimiz in situ katekolamin nano çiçekleri yüksek antimikrobiyal aktivite sergilemektedir. Bu özelliği sayesinde, yüzey kaplamaları olarak kullanılma potansiyeline sahiptir. Yüzeye bağlı sentez için, model yüzey olarak cam ve model organik bileşenler olarak katekolaminler (dopamin, epinefrin ve norepinefrin) seçilmiştir. İn situ katekolamin nano çiçekleri taramalı elektron mikroskobu ve temas açısı ölçümü kullanarak karakterize ettik. Ayrıca, in situ katekolamin nano çiçekleri kaplamalarının Staphylococcus aureus, Escherichia coli ve Candida albicans’a karşı antimikrobiyal aktivitesini test ettik. Sonuçlar, in situ katekolamin nano çiçekleri kaplamalarının antibakteriyel aktivitesinin önemli ölçüde arttığını ortaya koydu. Bulgular ışığında, antimikrobiyal yüzey kaplamalarının bir parçası olarak başarılı bir etki yaratmaları beklenmektedir.

Anahtar Kelimeler

Antibakteriyel kaplama , katekolaminler , nano çiçek

Etik Beyan

Bu çalışma etik kurul onayı gerektirmez. Klinik numune kullanılmamıştır.

Teşekkür

Bu araştırma herhangi bir finansman kuruluşundan/sektörden destek almamıştır.

Kaynakça

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