KANATLILARDA KULLANILAN SİPROFLOKSASİN İÇEREN İKİ FARKLI SPESİYALİTENİN ETÇİ PİLİÇLERDE BİYOEŞDEĞERLİLİKLERİNİN KARŞILAŞTIRILMASI

Yıl 2008, Cilt: 17 Sayı: 1, 23 - 31, 01.03.2008

Zeynep Soyer Sarıca Bilal Cem Liman

Öz

Bu çalışmada piyasada veteriner sağaltım amaçlı satılan iki farklı siprofloksasin preparatının biyoeşdeğerliğinin karşılaştırılması amaçlandı. Çalışmada, 30 günlük 21 adet dişi etçi piliç kullanıldı. Hayvanlar 30 gün boyunca aynı yemle beslenip aynı ortamda tutularak fizyolojik denklik sağlanan yetiştirme çiftliğinden temin edildi. Daha sonra tesadüfi olarak biri kontrol (Grup 1), ikisi deneme (Grup 2 ve Grup 3) olmak üzere üç gruba ayrıldı. 30. günün sonunda kontrol grubundaki piliçlere kanat altı venasından 5 mg/ kg dozunda siprofloksasin etken maddesi, diğer iki gruptaki hayvanlara ise iki farklı siprofloksasin preparatının her biri bir gruba olacak şekilde 5mg/ kg dozunda kursak içi yol ile verildi. İlaçların verilmesini takiben bütün gruplardaki hayvanlardan eş zamanlı olarak 0.08, 0.25, 0.50, 0.75, 1, 2, 4, 6, 8, 12 ve 24. saatlerde, kan örnekleri alındı ve alınan örneklerin serumları ayrıldı. Elde edilen serumlar, analizler gerçekleştirilinceye kadar -20°C derin dondurucuda saklandı. Daha sonra serum siprofloksasin konsantrasyonu agar jel difüzyon yöntemi kullanılarak tayin edildi. Farmakokinetik hesaplamalar, eşitlikleri esas alan farmakokinetik paket programda yapıldı. Elde edilen sonuçların istatistik analizleri SPSS 10. 0 istatistik paket programında gerçekleştirilerek verilere tek yönlü varyans analizi ve Duncan testi uygulandı. Damar içi uygulamayı takiben hızlı bir dağılım ve yavaş bir eliminasyon fazı gözlendi. Kursak içi uygulamayı takiben hızlı ve geniş ölçüde emilim gözlendi. Sonuç olarak; kursak içi uygulanan siprofloksasin müstahzarları, ortalama serum konsantrasyonları ve t½α, t½β, ortalama etki süresi,eğrinin altında kalan alan ,doruk konsantrasyon zamanı, doruk konsantrasyon ve mutlak biyoyararlanım olmak üzere farmakokinetik değişkenler değerlendirildiğinde ve aynı zamanda eğrinin altında kalan alan ,doruk konsantrasyon zamanı, doruk konsantrasyon değerleri referans preparatla karşılaştırıldığında, mutlak biyoyararlanım değerinin belirtilen %80-%120 aralığında olduğu , dorukkonsatrasyon zamanı ve doruk konsantrasyon değerlerinin ise belirtilen sınırın altında kaldığı gözlendi

Anahtar Kelimeler

Siprofloksasin, biyoeşdeğerlilik, etçi piliç

The Comparation of Bioequivalence of two Different Specialites Including Ciprofloxacin Used in Broiler Chickens

Öz

Aim of this study is to analyze the bio equality comparisons of two different specialty of ciprofloxacin used with the birds and chickens. In this work it is aimed to search the bio equality of two different preparations of veterinary medication sold in the market. 21 30 day-old broiler chicken is used in the work. Chickens were fed on the same food and they were kept under the same physiological conditions for 30 days. Later they were randomly chosen as one control group (group 1) and two experimental groups . At the end of the 30th day, chickens in the control group were given ciprofloxacin under wing vein with the dose of 5 kg /mg, and the other animals in the other two groups were given a different ciprofloxacin preparations in craw at the rate of 5 kg/mgr each . After the injection of medicine, blood samples of the animals in the all groups were taken on the 0.08 0.25 0.75 1 2 4 6 8 12 and 24 hours and the bloods were centrifuged for 5 minutes/at 3000 rpm and thus the serum was prepared. Obtained serums were kept under the -20 C deep freeze condition the analyzes were done. With the agar gel diffusion plasma ciprofloxacin concentration was determined. Pharmacokinetic calculations were done by the package application. Statistical analyzes of the obtained results are done by the SPSS 10. windows statistical application and single way variants analyzes and Duncan tests were applied to the data by this application. After application into the vine, fast distribution and slow elimination phase is seen. After the craw application, fast and widel absorption is seen. As a result, in craw application, when commercial preparations of ciprofloxacin are examined with average serum concentrations t½α, t½β, MRT, AUC, tmax, Cmax, F pharmacokinetic variables compared with AUC, tmax, Cmax values of referance preparations, F values can be seen between %80-%120 range; tmax, Cmax values are seen below the desired values

Anahtar Kelimeler

Ciprofloxacin, bioequivalence, broiler chicks

Kaynakça

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