KLİNİK ÖRNEKLERDE MOLEKÜLER YÖNTEMLERLE CHLAMYDIA TRACHOMATIS ARAŞTIRILMASI

Yıl 2013, Cilt: 22 Sayı: 2, 121 - 126, 01.06.2013

Osman Özüberk Selma Gökahmetoğlu

Öz

Chlamydia trachomatis, tüm dünyada enyaygın seksüel geçişli bakteriyel enfeksiyon ajanıolarak bilinir. Bu mikroorganizma zorunlu hücre içiparazitidir. enfeksiyonlarına, genitoüriner Lenfogranüloma venerum (LGV) suşları tarafındanoluşturulan ve özellikle genital bölgedeki lenfdüğümlerinde patoloji ile karakterize LGV’a nedenolmaktadır. Polimeraz Zincir Reaksiyonu (PCR) ve GerçekZamanlı PCR ile C. trachomatis’in araştırılmasıamaçlandı. arasında, Erciyes Üniversitesi Gevher NesibeAraştırma Hastalıkları ve Doğum poliklinikleri ile Ürolojipolikliniklerine genital akıntı ön tanısıyla başvuranve genital enfeksiyon düşünülen 50 hastadan alınansürüntü örnekleri çalışmaya dahil edildi. Çalışmayaalınan 50 sürüntü örneğinin 1’inde (%2) her ikiyöntem ile C. trachomatis-DNA pozitif bulundu.Sonuç olarak C. trachomatis DNA araştırılmasındaiki PCR yöntemi arasında fark bulunamadı

Anahtar Kelimeler

C. trachomatis, PCR, Gerçek

The Investigation of Chlamydia Trachomatis in Clinical Samples with Molecular Method

Öz

Chlamydia trachomatis is known as themost common bacterial infection agent to pass withsexual transition. This microorganism is anobligatory intracellular parasite. C. trachomatiscauses eye infections such as trachoma, pneumoniasin new born babies, genito urinary system infectionsand Lymphogranuloma venereum (LGV) that isconstituted by LGV strains causes pathology in thelymph nodes in the genital region. In this study, ithas been aimed to investigate C. trachomatis byusing polymerase chain reaction (PCR) and realtime PCR methods. The swab samples that havebeen taken from 50 patients, who have applied toErciyes University Gevher Nesibe Investigation andApplication Hospital Obstetric and GynecologyPoliclinics and Urology Policlinics with genitalflow pre-diagnosis between February 2010 andMarch 2010 dates, have been included in this study.C. trachomatis was found positive in 1 (2%) sampleof 50 samples. As a conclusion no difference wasfound between two PCR methods for investigation ofC. trachomatis DNA

Anahtar Kelimeler

C. trachomatis, PCR, Real Time PCR

Kaynakça

• Cheng H, Macaluso M, Vermund SH, et al. Relative accuracy of nucleic acid amplifica- tion test and culture in detecting Chlamydia in asymptomatic Men. J Clin Mic 2001;3927- 3937.
• Ferrero DV, Meyers HN, Schultz DE, et al. Performance of the Gen-Probe AMPLIFIED Chlamydia trachomatis assay in detecting Chlamydia trachomatis in endocervical and urine specimens from women and urethral and urine specimens from men attending Sexually Transmitted Disease and Family Planning Clinics. J Clin Microbiol 1998; 3230-3233.
• Olafsson JH, Davidsson S, Karlsson SM, et al. Diagnosis of Chlamydia trachomatis infection in high-risk females with PCR on first void urine. Acta Derm Venereol 1996; 76: 226- 227.
• Germain M, Alary M, Guedeme A, et al. Eva- luation of a screening algorithm for he diag- nosis of genital infections with Neisseria go- norrhoeae and Chlamydia trachomatis among female sexworkers in Benin. Sex Transm Dis 1997; 24: 109-115.
• Goessens WH, Mouton JW, van der Meijden WI, et al. Comparison of three commercially available amplification assays, AMP CT, LCx and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first void urine. J Clin Microbiol 1997; 35: 2628-2633.
• Puolakkainen M, Hiltunen-Back E, Reunala T, et al. Comparison of performances of two commercially available tests, a PCR assay and a ligase chain reaction test in detection of urogenital Chlamydia trachomatis infec- tion. J Clin Microbiol 1998; 36: 4489-4493.