BibTex RIS Kaynak Göster

ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE

Yıl 2018, Cilt: 27 Sayı: 1, 10 - 15, 01.03.2018

Öz

Scrophularia L. genus (Scrophulariaceae) has 310 taxa
worldwide. Some Scrophularia species are used in folk
medicine for the treatment of different skin inflammatory diseases and as antibacterial. The aim of the study
was to investigate the antimicrobial activity and determine the phenolic profile of S. kotscyhana Bentham, S.
cinarescens Boiss., S. catariifolia Boiss. & Heldr., S. chrysantha Jaub&Spach and S. scopolii var. adenocalyx
Hoppe. ex Pers. Phenolic components were analyzed by
an HPLC-DAD method. Disk diffusion method and microtiter-plate assay were used to determine the antimicrobial activities of the aerial parts methanolic extracts
of the species. Our results revealed that all of the tested
species contain caffeic acid and ferulic acid in varied
concentrations and none of them have chlorogenic acid.
All of the plant species exhibited moderate activity
against Candida albicans (MIC values; 0.3125 mg/mL).
Besides, S. cinarescens exhibited moderate activity
against Enterococcus faecalis (MIC value; 0.3125 mg/
mL) and S. chrysantha exhibited moderate activity
against Pseudomonas aeruginosa (MIC value; 0.3125
mg/mL). The results of this study showed that further
studies were needed to identify the secondary metabolites of the species and evaluate their antimicrobial activities.

Kaynakça

  • 1. Lall SS, Mill RR. Scrophularia L. In: Flora of Turkey and the East Aegean Islands, Davis PH (ed). Edinburgh University Press: Edinburgh 1978; 6:603-647.
  • 2. Olivencia AO. Scrophularia L. In: Flora Iberica, Benedí C, Rico E, Güemes J, Herrero A. (Eds.) Real Jardín Botánico, CSIC, Madrid 2009; 97−134.
  • 3. Uzunhisarcıklı ME, Güneri ED, Özbek F, et al. Scrophularia lucidaifolia (Scrophulariaceae), a new species from Turkey. Phytotaxa 2015; 204:95-98.
  • 4. Galindez JS, Lanza AMD, Matellano LF. Biologically active substances from the Genus Scrophularia. Pharm Biol 2002; 40:45-59.
  • 5. Nguyen AT, Fontaine J, Malonne H, et al. A sugar ester and an iridoid glycoside from Scrophularia ningpoensis. Phytochem 2005; 66:1186-1191.
  • 6. Shen X, Eichhorn T, Greten HJ, et al. Effects of Scrophularia ningpoensis Hemsl. on inhibition of proliferation, apoptosis induction and NFKB signaling of immortalized and cancer cell lines. Pharmaceuticals 2012; 5:189-208.
  • 7. Guarrera PM. Traditional phytotherapy in Central Italy (Marche, Abruzzo and Latium). Fitoterapia 2005; 76:1-25.
  • 8. Duke JA, Ayensu ES. Medicinal Plants of China, Reference Publications, Michigan 1985.
  • 9. Sezik E, Yeşilada E, Honda G, et al. Traditional medicine in Turkey X. Folk medicine in Central Anatolia. J of Ethnopharmacol 2001; 75:95-115.
  • 10. Altundağ E, Öztürk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Social and Behavioral Sci 2011; 19:756-777.
  • 11. Baydoun S, Lamis C, Helena D, et al. Ethnopharmacological survey of medicinal plants used in traditional medicine by the communities of Mount Hermon, Lebanon. J of Ethnopharmacol 2015; 173:139- 156.
  • 12. Swiatek L. Pharmacobotanical investigations on some species of family Scrophulariaceae. IV. Chemical constituents of the herbs of Scrophularia nodosa. Dissert Pharm Pharmacol 1970; 22:321-328.
  • 13. Swiatek L. Phenolic acids of underground parts of Scrophularia nodosa. Pol J Pharmacol Pharm 1973; 25:461-464.
  • 14. Fernández MA, García MD, Saenz MT. Antibacterial activity of the phenolic acids fractions of Scrophularia frutescens and Scrophularia sambucifolia. J Ethnopharmacol 1996; 53:11–14.
  • 15. Ahmad W, Khan MI, Waqar M, et al. In vitro Antibacterial Activity of Vitis vinifera Leaf Extracts against some Pathogenic Bacterial Strains. Adv Biol Res 2014; 8:62-67.
  • 16. Silva NCC, Fernandes JA. Biological properties of medicinal plants: a review of their antimicrobial activity. JVAT 2010; 16:402-413.
  • 17. Wiegand I, Hilpert K, Hancock REW. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols 2008; 3:163-175.
  • 18. Aliyazicioglu R, Demir S, Badem M Et al. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec Nat Prod 2017; 11:270-284.
  • 19. Kuete V. Potential of Cameroonian plants and derived products against microbial infections: A review. Planta Med 2010; 76:1479-1491.
  • 20. Fernandez MA, Saenz MT, Garcia MT. Antiinflammatory activity in rats and mice of phenolic acids isolated from Scrophularia frutescens. J Pharm Pharmacol 1998; 50:1183-1186.
  • 21. Jeong BR, Iyyakkannu S. Direct adventitious shoot regeneration, in vitro flowering, fruiting, secondary metabolite content and antioxidant activity of Scrophularia takesimensis Nakai. Plant Cell Tiss Org 2015; 123:607-618.
  • 22. Pasdaran A, Delazar A, Nazemiyeh H, et al. Chemical Composition, and Antibacterial (Against Staphylococcus aureus) and Free-Radical-Scavenging Activities of the Essential Oil of Scrophularia amplexicaulis Benth. Rec Nat Prod 2012; 6:350-355.
  • 23. Dulger B. Antimicrobial Activity of Some Endemic Scrophulariaceae from Turkey. Pharm Biol 2006; 44:672-676.
  • 24. Dulger B, Hacıoglu N. Antimicrobial Activity of Some Endemic Verbascum and Scrophularia Species from Turkey. Asian J Chem 2008; 20:3779-3785.
  • 25. Mahboubi M, Kazempour N, Nazar ARB. Total Phenolic, Total Flavonoids, Antioxidant and Antimicrobial Activities of Scrophularia striata Boiss Extracts. Jundishapur J Nat Pharm Prod 2013; 8:15-19.
  • 26. Li J, Huang X, Du X, et al. Study of chemical composition and antimicrobial activity of leaves and roots of Scrophularia ningpoensis. Nat Prod Res 2009; 23:775 -780.
  • 27. Bahmani M, Saatloo NV, Maghsoudi R, et al. A comparative study on the effect of ethanol extract of wild Scrophularia deserti and streptomycin on Brucellla melitensis. J Herb Med Pharmacol 2013; 2:17-20.
  • 28. J de Santos Galindez AM, Lanza D, Matellano F. Biologically active substances from the genus Scrophularia. Pharm Biol 2002; 40:45-49.
  • 29. Matejczyk M, Swislocka R, Golonko A, et al. Cytotoxic, genotoxic and antimicrobial activity of caffeic and rosmarinic acids and their lithium, sodium and potassium salts as potential anticancer compounds. Adv Med Sci 2018; 63:14-21.
  • 30. Pinho E, Soares G, Henriques M. Evaluation of antibacterial activity of caffeic acid encapsulated by βcyclodextrins. J Microencapsulation 2015; 32:804- 810.
  • 31. Borges A, Ferreira C, Saavedra MJ, Simoes M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Micr Drug Res 2013; 19:256-265.
  • 32. Ota AH, Abramovic VA, Ulrih NP. Interactions of pcoumaric, caffeic and ferulic acids and their styrenes with model lipid membranes. Food Chem 2011; 125:1256–1261.

Beş Scrophularia L. Türünün Antimikrobiyal Aktivitesi Ve Fenolik Profili

Yıl 2018, Cilt: 27 Sayı: 1, 10 - 15, 01.03.2018

Öz

Scrophularia L. cinsi (Scrophulariaceae) dünya üzerinde
310 taksona sahiptir. Bazı Scrophularia türleri halk arasında çeşitli iltihaplı cilt hastalıklarının tedavisinde ve
antibakteriyel olarak kullanılır. Çalışmanın amacı, S.
kotscyhana Bentham, S. cinarescens Boiss., S. catariifolia
Boiss. & Heldr., S. chrysantha Jaub&Spach ve S. scopolii
var. adenocalyx Hoppe. ex Pers. türlerinin
antimikrobiyal aktivitesini araştırmak ve fenolik profilini belirlemektir. Fenolik bileşenler bir HPLC-DAD yöntemi ile analiz edildi. Türlerin toprak üstü kısımlarının
metanol ekstrelerinin antimikrobiyal aktivitelerini belirlemek için disk difüzyon yöntemi ve mikrodilüsyon
yöntemi kullanıldı. Bulgularımız, test edilen tüm türlerin, çeşitli konsantrasyonlarda kafeik asit ve ferulik asit
içerdiğini ve hiçbirinin klorojenik asit içermediğini ortaya koymuştur. Çalışılan tüm türler, Candida albicans'a
(MIC değerleri; 0.3125 mg / mL) karşı orta derecede
aktivite göstermiştir. Ayrıca, S. cinarescens, Enterococcus
faecalis’e karşı (MIC değeri; 0.3125 mg/mL) ve S.
chrysantha Pseudomonas aeruginosa’ya karşı (MIC değeri; 0.3125 mg/mL) orta derecede aktivite gösterdi. Bu
çalışmanın sonuçları, türlerin sekonder metabolitlerinin
aydınlatılması ve antimikrobiyal etkinliklerinin değerlendirilmesi için daha ileri çalışmalara ihtiyaç duyulduğunu gösterdi.

Kaynakça

  • 1. Lall SS, Mill RR. Scrophularia L. In: Flora of Turkey and the East Aegean Islands, Davis PH (ed). Edinburgh University Press: Edinburgh 1978; 6:603-647.
  • 2. Olivencia AO. Scrophularia L. In: Flora Iberica, Benedí C, Rico E, Güemes J, Herrero A. (Eds.) Real Jardín Botánico, CSIC, Madrid 2009; 97−134.
  • 3. Uzunhisarcıklı ME, Güneri ED, Özbek F, et al. Scrophularia lucidaifolia (Scrophulariaceae), a new species from Turkey. Phytotaxa 2015; 204:95-98.
  • 4. Galindez JS, Lanza AMD, Matellano LF. Biologically active substances from the Genus Scrophularia. Pharm Biol 2002; 40:45-59.
  • 5. Nguyen AT, Fontaine J, Malonne H, et al. A sugar ester and an iridoid glycoside from Scrophularia ningpoensis. Phytochem 2005; 66:1186-1191.
  • 6. Shen X, Eichhorn T, Greten HJ, et al. Effects of Scrophularia ningpoensis Hemsl. on inhibition of proliferation, apoptosis induction and NFKB signaling of immortalized and cancer cell lines. Pharmaceuticals 2012; 5:189-208.
  • 7. Guarrera PM. Traditional phytotherapy in Central Italy (Marche, Abruzzo and Latium). Fitoterapia 2005; 76:1-25.
  • 8. Duke JA, Ayensu ES. Medicinal Plants of China, Reference Publications, Michigan 1985.
  • 9. Sezik E, Yeşilada E, Honda G, et al. Traditional medicine in Turkey X. Folk medicine in Central Anatolia. J of Ethnopharmacol 2001; 75:95-115.
  • 10. Altundağ E, Öztürk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Social and Behavioral Sci 2011; 19:756-777.
  • 11. Baydoun S, Lamis C, Helena D, et al. Ethnopharmacological survey of medicinal plants used in traditional medicine by the communities of Mount Hermon, Lebanon. J of Ethnopharmacol 2015; 173:139- 156.
  • 12. Swiatek L. Pharmacobotanical investigations on some species of family Scrophulariaceae. IV. Chemical constituents of the herbs of Scrophularia nodosa. Dissert Pharm Pharmacol 1970; 22:321-328.
  • 13. Swiatek L. Phenolic acids of underground parts of Scrophularia nodosa. Pol J Pharmacol Pharm 1973; 25:461-464.
  • 14. Fernández MA, García MD, Saenz MT. Antibacterial activity of the phenolic acids fractions of Scrophularia frutescens and Scrophularia sambucifolia. J Ethnopharmacol 1996; 53:11–14.
  • 15. Ahmad W, Khan MI, Waqar M, et al. In vitro Antibacterial Activity of Vitis vinifera Leaf Extracts against some Pathogenic Bacterial Strains. Adv Biol Res 2014; 8:62-67.
  • 16. Silva NCC, Fernandes JA. Biological properties of medicinal plants: a review of their antimicrobial activity. JVAT 2010; 16:402-413.
  • 17. Wiegand I, Hilpert K, Hancock REW. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols 2008; 3:163-175.
  • 18. Aliyazicioglu R, Demir S, Badem M Et al. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec Nat Prod 2017; 11:270-284.
  • 19. Kuete V. Potential of Cameroonian plants and derived products against microbial infections: A review. Planta Med 2010; 76:1479-1491.
  • 20. Fernandez MA, Saenz MT, Garcia MT. Antiinflammatory activity in rats and mice of phenolic acids isolated from Scrophularia frutescens. J Pharm Pharmacol 1998; 50:1183-1186.
  • 21. Jeong BR, Iyyakkannu S. Direct adventitious shoot regeneration, in vitro flowering, fruiting, secondary metabolite content and antioxidant activity of Scrophularia takesimensis Nakai. Plant Cell Tiss Org 2015; 123:607-618.
  • 22. Pasdaran A, Delazar A, Nazemiyeh H, et al. Chemical Composition, and Antibacterial (Against Staphylococcus aureus) and Free-Radical-Scavenging Activities of the Essential Oil of Scrophularia amplexicaulis Benth. Rec Nat Prod 2012; 6:350-355.
  • 23. Dulger B. Antimicrobial Activity of Some Endemic Scrophulariaceae from Turkey. Pharm Biol 2006; 44:672-676.
  • 24. Dulger B, Hacıoglu N. Antimicrobial Activity of Some Endemic Verbascum and Scrophularia Species from Turkey. Asian J Chem 2008; 20:3779-3785.
  • 25. Mahboubi M, Kazempour N, Nazar ARB. Total Phenolic, Total Flavonoids, Antioxidant and Antimicrobial Activities of Scrophularia striata Boiss Extracts. Jundishapur J Nat Pharm Prod 2013; 8:15-19.
  • 26. Li J, Huang X, Du X, et al. Study of chemical composition and antimicrobial activity of leaves and roots of Scrophularia ningpoensis. Nat Prod Res 2009; 23:775 -780.
  • 27. Bahmani M, Saatloo NV, Maghsoudi R, et al. A comparative study on the effect of ethanol extract of wild Scrophularia deserti and streptomycin on Brucellla melitensis. J Herb Med Pharmacol 2013; 2:17-20.
  • 28. J de Santos Galindez AM, Lanza D, Matellano F. Biologically active substances from the genus Scrophularia. Pharm Biol 2002; 40:45-49.
  • 29. Matejczyk M, Swislocka R, Golonko A, et al. Cytotoxic, genotoxic and antimicrobial activity of caffeic and rosmarinic acids and their lithium, sodium and potassium salts as potential anticancer compounds. Adv Med Sci 2018; 63:14-21.
  • 30. Pinho E, Soares G, Henriques M. Evaluation of antibacterial activity of caffeic acid encapsulated by βcyclodextrins. J Microencapsulation 2015; 32:804- 810.
  • 31. Borges A, Ferreira C, Saavedra MJ, Simoes M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Micr Drug Res 2013; 19:256-265.
  • 32. Ota AH, Abramovic VA, Ulrih NP. Interactions of pcoumaric, caffeic and ferulic acids and their styrenes with model lipid membranes. Food Chem 2011; 125:1256–1261.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA89YG28CS
Bölüm Araştırma Makalesi
Yazarlar

Gülin Renda Bu kişi benim

Büşra Korkmaz Bu kişi benim

Sercan Yıldırım Bu kişi benim

Ahu Reis Bu kişi benim

Nurdan Yazici Bektaş Bu kişi benim

Serhat Sevgi Bu kişi benim

Sevda Türkiş Bu kişi benim

M. Erkan Uzunhisarcıklı Bu kişi benim

Ahmet Yaşar Bu kişi benim

İlknur Tosun Bu kişi benim

Yayımlanma Tarihi 1 Mart 2018
Gönderilme Tarihi 1 Mart 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 27 Sayı: 1

Kaynak Göster

APA Renda, G., Korkmaz, B., Yıldırım, S., Reis, A., vd. (2018). ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE. Sağlık Bilimleri Dergisi, 27(1), 10-15.
AMA Renda G, Korkmaz B, Yıldırım S, Reis A, Bektaş NY, Sevgi S, Türkiş S, Uzunhisarcıklı ME, Yaşar A, Tosun İ. ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE. JHS. Mart 2018;27(1):10-15.
Chicago Renda, Gülin, Büşra Korkmaz, Sercan Yıldırım, Ahu Reis, Nurdan Yazici Bektaş, Serhat Sevgi, Sevda Türkiş, M. Erkan Uzunhisarcıklı, Ahmet Yaşar, ve İlknur Tosun. “ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE”. Sağlık Bilimleri Dergisi 27, sy. 1 (Mart 2018): 10-15.
EndNote Renda G, Korkmaz B, Yıldırım S, Reis A, Bektaş NY, Sevgi S, Türkiş S, Uzunhisarcıklı ME, Yaşar A, Tosun İ (01 Mart 2018) ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE. Sağlık Bilimleri Dergisi 27 1 10–15.
IEEE G. Renda, “ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE”, JHS, c. 27, sy. 1, ss. 10–15, 2018.
ISNAD Renda, Gülin vd. “ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE”. Sağlık Bilimleri Dergisi 27/1 (Mart 2018), 10-15.
JAMA Renda G, Korkmaz B, Yıldırım S, Reis A, Bektaş NY, Sevgi S, Türkiş S, Uzunhisarcıklı ME, Yaşar A, Tosun İ. ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE. JHS. 2018;27:10–15.
MLA Renda, Gülin vd. “ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE”. Sağlık Bilimleri Dergisi, c. 27, sy. 1, 2018, ss. 10-15.
Vancouver Renda G, Korkmaz B, Yıldırım S, Reis A, Bektaş NY, Sevgi S, Türkiş S, Uzunhisarcıklı ME, Yaşar A, Tosun İ. ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE. JHS. 2018;27(1):10-5.