ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE

Yıl 2018, Cilt: 27 Sayı: 1, 10 - 15, 01.03.2018

Gülin Renda Büşra Korkmaz Sercan Yıldırım Ahu Reis Nurdan Yazici Bektaş Serhat Sevgi Sevda Türkiş M. Erkan Uzunhisarcıklı Ahmet Yaşar İlknur Tosun

Öz

Scrophularia L. genus (Scrophulariaceae) has 310 taxa
worldwide. Some Scrophularia species are used in folk
medicine for the treatment of different skin inflammatory diseases and as antibacterial. The aim of the study
was to investigate the antimicrobial activity and determine the phenolic profile of S. kotscyhana Bentham, S.
cinarescens Boiss., S. catariifolia Boiss. & Heldr., S. chrysantha Jaub&Spach and S. scopolii var. adenocalyx
Hoppe. ex Pers. Phenolic components were analyzed by
an HPLC-DAD method. Disk diffusion method and microtiter-plate assay were used to determine the antimicrobial activities of the aerial parts methanolic extracts
of the species. Our results revealed that all of the tested
species contain caffeic acid and ferulic acid in varied
concentrations and none of them have chlorogenic acid.
All of the plant species exhibited moderate activity
against Candida albicans (MIC values; 0.3125 mg/mL).
Besides, S. cinarescens exhibited moderate activity
against Enterococcus faecalis (MIC value; 0.3125 mg/
mL) and S. chrysantha exhibited moderate activity
against Pseudomonas aeruginosa (MIC value; 0.3125
mg/mL). The results of this study showed that further
studies were needed to identify the secondary metabolites of the species and evaluate their antimicrobial activities.

Anahtar Kelimeler

Antimicrobial activity, HPLC-DAD, microtiter-plate assay, medicinal plants, Scrophularia species.

Beş Scrophularia L. Türünün Antimikrobiyal Aktivitesi Ve Fenolik Profili

Öz

Scrophularia L. cinsi (Scrophulariaceae) dünya üzerinde
310 taksona sahiptir. Bazı Scrophularia türleri halk arasında çeşitli iltihaplı cilt hastalıklarının tedavisinde ve
antibakteriyel olarak kullanılır. Çalışmanın amacı, S.
kotscyhana Bentham, S. cinarescens Boiss., S. catariifolia
Boiss. & Heldr., S. chrysantha Jaub&Spach ve S. scopolii
var. adenocalyx Hoppe. ex Pers. türlerinin
antimikrobiyal aktivitesini araştırmak ve fenolik profilini belirlemektir. Fenolik bileşenler bir HPLC-DAD yöntemi ile analiz edildi. Türlerin toprak üstü kısımlarının
metanol ekstrelerinin antimikrobiyal aktivitelerini belirlemek için disk difüzyon yöntemi ve mikrodilüsyon
yöntemi kullanıldı. Bulgularımız, test edilen tüm türlerin, çeşitli konsantrasyonlarda kafeik asit ve ferulik asit
içerdiğini ve hiçbirinin klorojenik asit içermediğini ortaya koymuştur. Çalışılan tüm türler, Candida albicans'a
(MIC değerleri; 0.3125 mg / mL) karşı orta derecede
aktivite göstermiştir. Ayrıca, S. cinarescens, Enterococcus
faecalis’e karşı (MIC değeri; 0.3125 mg/mL) ve S.
chrysantha Pseudomonas aeruginosa’ya karşı (MIC değeri; 0.3125 mg/mL) orta derecede aktivite gösterdi. Bu
çalışmanın sonuçları, türlerin sekonder metabolitlerinin
aydınlatılması ve antimikrobiyal etkinliklerinin değerlendirilmesi için daha ileri çalışmalara ihtiyaç duyulduğunu gösterdi.

Anahtar Kelimeler

Antimikrobiyal aktivite, HPLCDAD, mikrodilüsyon yöntemi, tıbbi bitkiler, Scrophularia türleri

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