INVESTIGATION OF ENZYME INHIBITORY PROPERTIES AND ANTIOXIDANT ACTIVITY OF CAPPARIS OVATA DESF. VAR. PALAESTINA ZOH. FLOWER AND FRUIT EXTRACTS

Yıl 2021, Cilt: 30 Sayı: 2, 113 - 117, 15.09.2021

Leyla Paşayeva Ayşe Kübra Karaboğa Arslan

https://doi.org/10.34108/eujhs.916214

Öz

In this study, the inhibiting effect on α-amylase and α-glucosidase and the antioxidant activity of ethanol extracts from flower and fruit of Capparis ovata var. palaestina was investigated. The antioxidant activity of extracts was tested with DPPH●, ABTS●+ and FRAP tests. According to the results, the highest α-glucosidase and α-amylase activity were found in fruit extract (IC50=271.72±0.41 µg/mL and 142.07±0.85 µg/mL, respectively). Also, the highest TPC (26.40±0.02mgGAE/gextract) and TFC (8.33±0.30 mgCA /gextract) was found in the same extract and this extract showed the highest DPPH● activity (%32). Although, flower extract showed the highest FRAP values (at a concentration of 1 mg/mL equivalent to 262.69±2.27 mmol Fe2+) and ABTS+● (at a concentration of 0.5 mg/mL equivalent to 0.18±0.01 µM Trolox) activity. In conclusion, it is thought that these studies will be shed light on future studies on this species.

Anahtar Kelimeler

Capparaceae, Diabetes, DPPH, α-amylase, α-glucosidase

Teşekkür

The authors are thankful to Kübra Olagan for providing plant material.

CAPPARIS OVATA DESF. VAR. PALAESTINA ZOH. BİTKİSİNİN ÇİÇEK VE MEYVE EKSTRELERİNİN ENZİM İNHİBİTÖR ÖZELLİKLERİ VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI

Öz

Bu çalışmada Capparis ovata var. palaestina bitkisinin çiçek ve meyvelerinden hazırlanan etanol ekstresinin α-amilaz ve α-glikozidaz inhibitör etkisi ve antioksidan özelliği araştırılmıştır. Ekstrelerin antioksidant etkisi DPPH●, ABTS●+ ve FRAP testleri kullanılarak değerlerndirilmiştir. Sonuçlara göre en yüksek α-amilaz ve α-glikozidaz etki meyve ekstresinde bulunmuştur (Sırasıyla, IC50=271.72±0.41 µg/ml and 142.07±0.845 µg/ml). Ayrıca en yüksek TPC (26.40±0.02mgGAE/gextract) ve TFC (8.33±0.30 mgCA /gextract) değerleri ve DPPH● (32%) etkisi de aynı ekstrede görülmüştür. Bununla birlikte çiçek ekstresi en yüksek FRAP değerleri (1 mg/ml konsantrasyonda 262.69±2.27 mmol Fe2+ eşdeğer) ve ABTS+● (0,5 mg/ml konsantrasyonda 0.18±0.01 µM Troloks’a eşdeğer) aktivite göstermiştir. Sonuç olarak, yapılan çalışmanın bu bitki üzerinde ileride yapılacak çalışmalara ışık tutacağı düşünülmektedir.

Anahtar Kelimeler

Capparaceae, Diyabet, DPPH, α-amilaz, α-glikozidaz

Kaynakça

• 1. Maritim AC, Sanders RA, Watkins JB, 3rd. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 2003; 17 (1): 24-38.
• 2. Surampudi PN, John-Kalarickal J, Fonseca VA. Emerging concepts in the pathophysiology of type 2 diabetes mellitus. Mt Sinai J Med. 2009; 76 (3): 216-226.
• 3. Hung HY, Qian K, Morris-Natschke SL, et al. Recent discovery of plant-derived anti-diabetic natural products. Nat Prod Rep. 2012; 29 (5): 580-606.
• 4. Sloop KW, Michael MD, Moyers JS. Glucagon as a target for the treatment of type 2 diabetes. Expert Opin Ther Targets. 2005; 9 (3): 593-600.
• 5. Okur ME, Polat DC, Ozbek H, et al. Evaluation of the antidiabetic property of Capparis Ovata Desf. Var. Palaestina Zoh. Extracts using in vivo and in vitro approaches. Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders). 2018; 18 (5): 489-501.
• 6. Okur ME, Özbek H, Polat DÇ, et al. Hypoglycemic activity of Capparis ovata desf. var. palaestina zoh. methanol extract. Brazilian Journal of Pharmaceutical Sciences. 2018; 54 (3).
• 7. Aghel N, Rashidi I, Mombeini A. Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice. 2007.
• 8. Muhaidat R, Al-Qudah MA, Al-Shayeb A, et al. Chemical profile and antibacterial activity of crude fractions and essential oils of Capparis ovata Desf. and Capparis spinosa L.(Capparaceae). Int J Integr Biol. 2013; 14 (1): 39.
• 9. Okur ME, Ayla Ş, Çiçek Polat D, et al. Novel insight into wound healing properties of methanol extract of Capparis ovata Desf. var. palaestina Zohary fruits. Journal of Pharmacy and Pharmacology. 2018; 70 (10): 1401-1413.
• 10. Ergur BU, Gazioglu I, Sen A, et al. Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice. 2016.
• 11. Arslan R, Bektas N. Antinociceptive effect of methanol extract of Capparis ovata in mice. Pharm Biol. 2010; 48 (10): 1185-1190.
• 12. Arslan R, Bektas N, Ozturk Y. Antinociceptive activity of methanol extract of fruits of Capparis ovata in mice. J Ethnopharmacol. 2010; 131 (1): 28-32.
• 13. Tlili N, Elfalleh W, Saadaoui E, et al. The caper (Capparis L.): ethnopharmacology, phytochemical and pharmacological properties. Fitoterapia. 2011; 82 (2): 93-101.
• 14. Gull T, Anwar F, Sultana B, et al. Capparis species: A potential source of bioactives and high-value components: A review. Industrial Crops and Products. 2015; 67: 81-96.
• 15. Paşayeva L, Özalp B, Fatullayev H. Potential enzyme inhibitory properties of extracts and fractions from fruit latex of Ficus carica-based on inhibition of α-amylase and α-glucosidase. Journal of Food Measurement and Characterization. 2020; 14 (5): 2819-2827.
• 16. Gyamfi MA, Aniya Y. Antioxidant properties of Thonningianin A, isolated from the African medicinal herb, Thonningia sanguinea. Biochem Pharmacol. 2002; 63 (9): 1725-1737.
• 17. Devasahayam G, Burke DJ, Sturgill TW. Golgi manganese transport is required for rapamycin signaling in Saccharomyces cerevisiae. Genetics. 2007; 177 (1): 231-238.