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Mikrosatellit lokuslarının tavuk ve güvercinlerde karşılaştırmalı analizi

Yıl 2015, Cilt: 31 Sayı: 1, 43 - 50, 01.03.2015

Gülseren Yıldız Öz Sema Çakır İsmail Kurhan Müge Doğan Elif Şahin Mehmet Nizamlıoğlu Ercan Kurar

Öz

Amaç: Bu çalışmanın amacı, tavuk mikrosatellit markörlerinin güvercin (Columba livia) genetik çalışmaları için kullanılabilirliğinin araştırılmasıdır.Gereç ve Yöntem: Toplam 100 adet tavuk mikrosatellit markörü tavuk bağlantı haritalarından seçildi. Güvercin ve pozitif kontrol olarak tavuk DNA’sı kullanılarak polimeraz zincir reaksiyonu (PZR) ile spesifik genom bölgeleri çoğaltıldı. PZR ürünleri kapiller elektroforez ile ayrıştırıldı ve allel genotipleri tespit edildi. Pilot bir çalışma ile 24 adet pozitif mikrosatellitin genel populasyon parametrelerinden allel sayısı (Na), gözlenen (Ho) ve beklenen (He) heterezigotluk ile HardyWeinberg Dengesi’nden (HWE) sapma değerleri güvercin populasyonunda değerlendirildi.Bulgular: Toplam 48 mikrosatellit lokusu güvercin DNA’sında (%48) PZR ile yükseltgenmiştir. Pilot çalışma kapsamında 24 lokusun ortalama allel sayısı 2.5 olup allel sayısı 1-5 arasında değişmektedir. Toplam 60 farklı allel tespit edilmiştir. Ho ve He değerleri sırasıyla 0.000-1.000 ve 0.000-0.698 arasında değişmektedir. Öneri: Genel olarak gözlenen allel sayısı ve polimorfizm değerleri düşük olmasına rağmen, tavuk mikrosatellit lokuslarının güvercin genetik çalışmalarında kullanılabileceği kanaatine varılmıştır.

Anahtar Kelimeler

Güvercin tavuk mikrosatellit markör polimorfizm

Kaynakça

  • Ando H, Kaneko S, Suzuki H, et al., 2011. Genetic diversity of the Japanese Wood Pigeon, Columba janthina, endemic to islands of east Asia, estimated by newly developed microsatellite markers. Zool Sci, 28, 891-896.
  • Anonim 2004. http://www.nhgri.nih.gov/11510730. Chicken genome assembled. Erişim tarihi; 15.09.2014.
  • Anonim 2014. Pigeon genetics at the University of Utah. http://biologylabs.utah.edu/shapiro/shapiro_lab/pigeons.html. Erişim tarihi; 15.09.2014.
  • Bidgood JJ, Somes RGJ, 1993. Genemap of the chicken (Gallus gallus), in: Genetic maps, Ed; O’Brien S, 6th edition, Cold Springs Harbor Laboratory Press, Cold Springs Harbor, USA, pp: 4.333-4.442.
  • Bumstead N, Palya J, 1992. A preliminary linkage map of the chicken genome. Genomics, 13, 690-697.
  • Bulut Z, Kurar E, Ozsensoy Y, et al., 2013. Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations. Eurasian J Vet Sci, 29, 30-38.
  • Burt DW, Bruley C, Dunn IC, 1999. The dynamics of chromosome evolution in birds. Nature, 402, 411-413.
  • de Gortari MJ, Freking BA, Kappes SM, et al., 1996. Extensive genomic conservation of cattle microsatellite heterozygosity in sheep. Anim Genet, 28, 274-290.
  • Don RH, Cox PT, Wainwright BJ, et al., 1991. Touchdown PCR to circumvent spurious priming during gene amplification. Nucl Acid Res, 19, 4008.
  • Fields RL, Scribner KT, 1997. Isolation and characterization of novel waterfowl microsatellite loci: Cross-species comparisons and research applications. Mol Ecol, 6, 199-202.
  • Groenen MA, Crooijmans RP, Veenendaal A, et al., 1998. A comprehensive microsatellite linkage map of the chicken genome. Genomics, 49, 265-274.
  • Groenen MA, Cheng HH, Bumstead N, et al., 2000. A consensus linkage map of the chicken genome. Genome Res, 10, 137-147.
  • Hanotte O, Puch A, Maucher C, et al., 1997. Nine novel chicken microsatellite loci and their utility in other Galliformes. Anim Genet, 28, 308-322.
  • Horbanczuk JO, Kawka M, Sacharczuk M, et al., 2007. A search for sequence similarity between chicken (Gallus domesticus) and ostrich (Struthio camellus) microsatellite markers. Anim Sci Pap Rep, 4, 283-288.
  • Huang Y, Tu J, Cheng X, et al., 2005. Characterization of 35 novel microsatellite DNA markers from the duck (Anas platyrhynchos) genome and cross-amplification in other birds. Genet Sel Evol, 37, 455-472.
  • Hutt FB, 1936. Genetics of fowl. VI. A tentative chromosome map. Neue Forsch Tierzucht Abstam (Duerst Festschrift) pp: 105-112.
  • Kappes SM, Keele JW, Stone RT, et al., 1997. A second generation map of the bovine genome. Genome Res, 7, 235-249.
  • Kayang BB, Inoue-Murayama M, Hoshi T, et al., 2002. Microsatellite loci in Japanese quail and cross-species amplification in chicken and quinea fowl. Genet Sel Evol, 34, 233- 253.
  • Levin I, Santangelo L, Cheng H, 1994. An autosomal genetic linkage map of the chicken. J Hered, 85, 79-85.
  • Maddox JF, Davies KP, Crawford AM, et al., 2001. An enhanced linkage map of the sheep genome comprising more than 1000 loci. Genom Res, 11, 1275-1289.
  • Mukesh, Sathyakumar S, 2011. Eighteen polymorphic microsatellites for domestic pigeon Columba livia var. domestica developed by cross species amplification of chicken markers. J Genet, 90, 86-89.
  • O'Brien SJ, Womack JE, Lyons LA, et al., 1993. Anchored reference loci for comparative genome mapping in mammals. Nat Genet, 3, 103-112.
  • Özşensoy Y, Kurar E, 2012. Markör sistemleri ve genetik karakterizasyon çalışmalarında kullanımları. J Cell Mol Biol, 10, 11-19.
  • Peakall R, Smouse PE, 2006. GenAlEx6: Genetic analysis in Excel, Population genetic software for teaching and research. Mol Ecol Notes, 6, 288-295.
  • Petren K, 1998. Microsatellite primers from Geospiza fortis and cross-species amplification in Darwin’s finches. Mol Ecol, 7, 1782-1784.
  • Primmer CR, Moller AP, Ellegren H, 1996. A wide-range survey of cross-species microsatellite amplification in birds. Mol Ecol, 5, 365-378.
  • Reed KM, Mendoza KM, Beattie CW, 2000. Comparative analysis of microsatellite loci in chicken and turkey. Genome, 43, 796-802.
  • Sambrook J, Fritsch EF, Maniatis T, 1989. Molecular Clonning: A Laboratory Manual. Second Edition, Volume 2, ColdSpring Harbor, New York, USA, pp: 9.16-9.19.
  • Schmutz J, Grimwood J, 2004. Fowl sequence. Nature, 432, 679-680.
  • Traxler B, Brem G, Muller M, et al., 2000. Polymorphic DNA microsatellites in the domestic pigeon, Columba livia var. domestica. Mol Ecol, 9, 365–378.
  • Weber JL, May PE, 1989. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet, 44, 388-396.
  • Womack JE, Kata SR, 1995. Bovine genome mapping: evolutionary inference and the power of comparative genomics. Curr Op Gen Develop, 5, 725-733.
  • Yılmaz O, Ertuğrul M, 2012. Tarihte güvercin yetiştiriciliğinin önemi. J Agric Fac HRU, 16, 1-7.

Comparative analysis of microsatellite loci in chicken and pigeon

Yıl 2015, Cilt: 31 Sayı: 1, 43 - 50, 01.03.2015

Gülseren Yıldız Öz Sema Çakır İsmail Kurhan Müge Doğan Elif Şahin Mehmet Nizamlıoğlu Ercan Kurar

Öz

Aim: The aim of this study is to investigate utilization of chicken microsatellite loci in genetic studies of pigeon (Columba livia) populations.Materials and Methods: A total of chicken 100 microsatellite markers were selected from chicken linkage maps. DNAs of pigeons and a chicken as a positive control were used to amplify specific genomic regions by polymerase chain reaction (PCR). The resulting PCR products were separated by capillary electrophoresis and allele genotypes were determined. In a pilot study, general population parameters including number of allele (Na), observed (Ho) and expected (He) heterozygosities and deviation from Hardy-Weinberg Equilibrium (HWE) were calculated for 24 positive loci in a pigeon population.Results: A total of 48 microsatellite loci were amplified (48%) in pigeon DNA and expected PCR products were observed. In the pilot study, alleles numbers varied 1-5 and mean Na was 2.5 for 24 loci. A total of 60 different alleles were determined. Ho and He values were observed as 0.0001.000 and 0.000-0.698, respectively.Conclusion: Although Na and polymorphism levels were lower in general, results of this study suggested that chicken microsatellite loci can be used in genetic studies of pigeons.

Anahtar Kelimeler

Pigeon chicken microsatellite marker polymorphism

Kaynakça

  • Ando H, Kaneko S, Suzuki H, et al., 2011. Genetic diversity of the Japanese Wood Pigeon, Columba janthina, endemic to islands of east Asia, estimated by newly developed microsatellite markers. Zool Sci, 28, 891-896.
  • Anonim 2004. http://www.nhgri.nih.gov/11510730. Chicken genome assembled. Erişim tarihi; 15.09.2014.
  • Anonim 2014. Pigeon genetics at the University of Utah. http://biologylabs.utah.edu/shapiro/shapiro_lab/pigeons.html. Erişim tarihi; 15.09.2014.
  • Bidgood JJ, Somes RGJ, 1993. Genemap of the chicken (Gallus gallus), in: Genetic maps, Ed; O’Brien S, 6th edition, Cold Springs Harbor Laboratory Press, Cold Springs Harbor, USA, pp: 4.333-4.442.
  • Bumstead N, Palya J, 1992. A preliminary linkage map of the chicken genome. Genomics, 13, 690-697.
  • Bulut Z, Kurar E, Ozsensoy Y, et al., 2013. Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations. Eurasian J Vet Sci, 29, 30-38.
  • Burt DW, Bruley C, Dunn IC, 1999. The dynamics of chromosome evolution in birds. Nature, 402, 411-413.
  • de Gortari MJ, Freking BA, Kappes SM, et al., 1996. Extensive genomic conservation of cattle microsatellite heterozygosity in sheep. Anim Genet, 28, 274-290.
  • Don RH, Cox PT, Wainwright BJ, et al., 1991. Touchdown PCR to circumvent spurious priming during gene amplification. Nucl Acid Res, 19, 4008.
  • Fields RL, Scribner KT, 1997. Isolation and characterization of novel waterfowl microsatellite loci: Cross-species comparisons and research applications. Mol Ecol, 6, 199-202.
  • Groenen MA, Crooijmans RP, Veenendaal A, et al., 1998. A comprehensive microsatellite linkage map of the chicken genome. Genomics, 49, 265-274.
  • Groenen MA, Cheng HH, Bumstead N, et al., 2000. A consensus linkage map of the chicken genome. Genome Res, 10, 137-147.
  • Hanotte O, Puch A, Maucher C, et al., 1997. Nine novel chicken microsatellite loci and their utility in other Galliformes. Anim Genet, 28, 308-322.
  • Horbanczuk JO, Kawka M, Sacharczuk M, et al., 2007. A search for sequence similarity between chicken (Gallus domesticus) and ostrich (Struthio camellus) microsatellite markers. Anim Sci Pap Rep, 4, 283-288.
  • Huang Y, Tu J, Cheng X, et al., 2005. Characterization of 35 novel microsatellite DNA markers from the duck (Anas platyrhynchos) genome and cross-amplification in other birds. Genet Sel Evol, 37, 455-472.
  • Hutt FB, 1936. Genetics of fowl. VI. A tentative chromosome map. Neue Forsch Tierzucht Abstam (Duerst Festschrift) pp: 105-112.
  • Kappes SM, Keele JW, Stone RT, et al., 1997. A second generation map of the bovine genome. Genome Res, 7, 235-249.
  • Kayang BB, Inoue-Murayama M, Hoshi T, et al., 2002. Microsatellite loci in Japanese quail and cross-species amplification in chicken and quinea fowl. Genet Sel Evol, 34, 233- 253.
  • Levin I, Santangelo L, Cheng H, 1994. An autosomal genetic linkage map of the chicken. J Hered, 85, 79-85.
  • Maddox JF, Davies KP, Crawford AM, et al., 2001. An enhanced linkage map of the sheep genome comprising more than 1000 loci. Genom Res, 11, 1275-1289.
  • Mukesh, Sathyakumar S, 2011. Eighteen polymorphic microsatellites for domestic pigeon Columba livia var. domestica developed by cross species amplification of chicken markers. J Genet, 90, 86-89.
  • O'Brien SJ, Womack JE, Lyons LA, et al., 1993. Anchored reference loci for comparative genome mapping in mammals. Nat Genet, 3, 103-112.
  • Özşensoy Y, Kurar E, 2012. Markör sistemleri ve genetik karakterizasyon çalışmalarında kullanımları. J Cell Mol Biol, 10, 11-19.
  • Peakall R, Smouse PE, 2006. GenAlEx6: Genetic analysis in Excel, Population genetic software for teaching and research. Mol Ecol Notes, 6, 288-295.
  • Petren K, 1998. Microsatellite primers from Geospiza fortis and cross-species amplification in Darwin’s finches. Mol Ecol, 7, 1782-1784.
  • Primmer CR, Moller AP, Ellegren H, 1996. A wide-range survey of cross-species microsatellite amplification in birds. Mol Ecol, 5, 365-378.
  • Reed KM, Mendoza KM, Beattie CW, 2000. Comparative analysis of microsatellite loci in chicken and turkey. Genome, 43, 796-802.
  • Sambrook J, Fritsch EF, Maniatis T, 1989. Molecular Clonning: A Laboratory Manual. Second Edition, Volume 2, ColdSpring Harbor, New York, USA, pp: 9.16-9.19.
  • Schmutz J, Grimwood J, 2004. Fowl sequence. Nature, 432, 679-680.
  • Traxler B, Brem G, Muller M, et al., 2000. Polymorphic DNA microsatellites in the domestic pigeon, Columba livia var. domestica. Mol Ecol, 9, 365–378.
  • Weber JL, May PE, 1989. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet, 44, 388-396.
  • Womack JE, Kata SR, 1995. Bovine genome mapping: evolutionary inference and the power of comparative genomics. Curr Op Gen Develop, 5, 725-733.
  • Yılmaz O, Ertuğrul M, 2012. Tarihte güvercin yetiştiriciliğinin önemi. J Agric Fac HRU, 16, 1-7.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA59ZJ53BA
Bölüm Araştırma
Yazarlar

Gülseren Yıldız Öz Bu kişi benim

Sema Çakır Bu kişi benim

İsmail Kurhan Bu kişi benim

Müge Doğan Bu kişi benim

Elif Şahin Bu kişi benim

Mehmet Nizamlıoğlu Bu kişi benim

Ercan Kurar Bu kişi benim

Yayımlanma Tarihi 1 Mart 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 31 Sayı: 1

Kaynak Göster

APA Öz, G. Y., Çakır, S., Kurhan, İ., Doğan, M., vd. (2015). Comparative analysis of microsatellite loci in chicken and pigeon. Eurasian Journal of Veterinary Sciences, 31(1), 43-50.
AMA Öz GY, Çakır S, Kurhan İ, Doğan M, Şahin E, Nizamlıoğlu M, Kurar E. Comparative analysis of microsatellite loci in chicken and pigeon. Eurasian J Vet Sci. Mart 2015;31(1):43-50.
Chicago Öz, Gülseren Yıldız, Sema Çakır, İsmail Kurhan, Müge Doğan, Elif Şahin, Mehmet Nizamlıoğlu, ve Ercan Kurar. “Comparative Analysis of Microsatellite Loci in Chicken and Pigeon”. Eurasian Journal of Veterinary Sciences 31, sy. 1 (Mart 2015): 43-50.
EndNote Öz GY, Çakır S, Kurhan İ, Doğan M, Şahin E, Nizamlıoğlu M, Kurar E (01 Mart 2015) Comparative analysis of microsatellite loci in chicken and pigeon. Eurasian Journal of Veterinary Sciences 31 1 43–50.
IEEE G. Y. Öz, S. Çakır, İ. Kurhan, M. Doğan, E. Şahin, M. Nizamlıoğlu, ve E. Kurar, “Comparative analysis of microsatellite loci in chicken and pigeon”, Eurasian J Vet Sci, c. 31, sy. 1, ss. 43–50, 2015.
ISNAD Öz, Gülseren Yıldız vd. “Comparative Analysis of Microsatellite Loci in Chicken and Pigeon”. Eurasian Journal of Veterinary Sciences 31/1 (Mart 2015), 43-50.
JAMA Öz GY, Çakır S, Kurhan İ, Doğan M, Şahin E, Nizamlıoğlu M, Kurar E. Comparative analysis of microsatellite loci in chicken and pigeon. Eurasian J Vet Sci. 2015;31:43–50.
MLA Öz, Gülseren Yıldız vd. “Comparative Analysis of Microsatellite Loci in Chicken and Pigeon”. Eurasian Journal of Veterinary Sciences, c. 31, sy. 1, 2015, ss. 43-50.
Vancouver Öz GY, Çakır S, Kurhan İ, Doğan M, Şahin E, Nizamlıoğlu M, Kurar E. Comparative analysis of microsatellite loci in chicken and pigeon. Eurasian J Vet Sci. 2015;31(1):43-50.