Geçmişten günümüze Türkiye’de dolaşımda olan Canine adenovirus tip 2 suşlarının genetik incelemesi

Öz

Canine adenoviruslar (CAV) hem evcil hem de vahşi yaşamda köpek ve tilkilerde enfeksiyona neden olabilmektedir. Canine Adenovirus Tip-1 (CAV-1) infectious canine hepatitis (ICH) enfeksiyonuna ve Canine Adenovirus Tip-2 (CAV-2) infectious canine laryngotracheitis enfeksiyonuna neden olmaktadır. Virusun epidemiyolojisi çoğu bölgede yeterince net değildir. Bu amaçla Türkiye'de on yıllık süre zarfında dolaşımda olan suşlar moleküler düzeyde incelendi ve genetik bir heterojenlik olduğu görüldü. Bu incelemeyi yapabilmek için 2011 yılında ishalli barınak köpeklerinden toplanan 32 adet dışkı örneği kullanıldı. İlk olarak, MDCK hücre hattında virus izolasyonu yapıldı ve 4/32 örnekte sitopatojenik etkiler (CPE) gözlendi. İkinci olarak, CPE pozitif dört izolat CAV-2'nin E3 geni yönünden PCR ile pozitif bulundu. Sekans işlemi sonucu elde edilen diziler filogenetik ağaçta aynı alt grup altındaki diğer Türk izolatlarından farklı bir dal üzerine yerleşti. Kısmi E3 gen analizine göre, suşlar arasında yedi amino asitte değişim tespit edildi. Shannon entropi değeri ve ConSurf analizine göre tüm amino asit değişikliklerinin önemli antijenik bölgelerde meydana geldiği belirlendi. Bu çalışma, Türkiye CAV-2 suşları arasındaki olası genetik heterojeniteye dikkat çekmektedir.

Anahtar Kelimeler

• Canine adenovirus tip 2
• moleküler karakterizasyon
• virus izolasyonu

Genetic analysis of Canine adenovirus type 2 strains circulating in Turkey from past to present

Öz

Canine adenoviruses (CAV) can infect both domestic and wild canid. Canine Adenovirus Type-1 (CAV-1) and Canine Adenovirus Type-2 (CAV-2) are the cause of infectious canine hepatitis and infectious canine laryngotracheitis, respectively. The epidemiology of the virus is not clear enough in the most territory. For this purpose, data on the virus in Turkey, which is a bridge between continents, were examined. Comparing the sequence data of strains circulated in the field over a ten-year period revealed a genetic heterogeneity. To make this comparision total of 32 fecal samples collected from shelter dogs with diarrhea in 2011 was used. Firstly, virus isolation was performed in MDCK cells and cytopathogenic effects were observed in 4/32 samples. Secondly, the supernatant of four isolates were found positive for the E3 gene of CAV-2 by PCR. Strains were placed on a different branch from other Turkish isolates under the same subgroup in the phylogenetic tree. According to partial E3 gene analysis, seven amino acid substitutions were detected between the strains. Due to Shannon entropy value and ConSurf analysis, all of them were indicated as important antigenic sites. This study pointed out the possible genetic heterogeneity among CAV-2 in Turkey.

Anahtar Kelimeler

• Canine adenovirus type 2
• molecular characterization
• virus isolation

Kaynakça

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