Monoklanal Antikorların Newcastle Hastalığının Teşhis ve Epidemiyolojisinde Kullanımı

Öz

Poliklonal antikorların konvansiyonel serolajik testlerde virusların ayrımında kullanılması bazı problemlere yol açmaktadır. Monoklonal antikor teknolojisi (mAb) konu için yeni bir alternatif olmuştur. Newcastle Hastalığı Viruslarına (NHVJ karşı hazırlanan mAb'ler değişik izolatlar arasındaki kayda değer antijenik farklılığı göstermişlerdir. NHV suşlarının hepsi yada pek çoğu için spesifik olan ve HI aktivitesi gösteren mAb'ler. Newcastle Hastalığı (NH) için referans laboratuvar olan Weybridge'de rutin teşhiste kullanılmaktadır. Bu mAb'lerin kullanımı NHV. variant NHV. Lasota ve PMV-3 arasında ayrımı sağlamaktadır. MAb'ler aynı zamanda NHV'larının antijenik yapılarına göre gruplanmalarını sağlamışlardır. Bu konudaki en başarılı yaklaşım Russell ve Alexander ' ın çalışmaları olmuştur. Araştırıcılar, NHV'larını, NHV Ulster 2C suşuna karşı oluşturulan 9 mAb ile reaksiyonlarını temel alarak gruplamışlardır. Oluşturulan grupların virulans, temel konakçı gibi epidemiyolojikal özellikleri büyük ölçüde paylaştığı görülmüştür. MAb gruplamasının NH'nin epidemiyolojisindeki başarısı. İngiltere'de variant NHV'larının tavuklarda oluşturduğu NH salgınlarını ve Türkiye'de Güney Marmara bölgesinde son zamanlarda görülen NH salgınını da içeren pek çok olguda kanıtlanmıştır.

Anahtar Kelimeler

• Etlik Veteriner Mikrobiyoloji Dergisi
• Newcastle
• Monoklonal Antikorlar
• Epidemiyoloji
• 1999

Use of Monoclonal Antibiotics in Diagnosis And Epidemiology or Newcastle Disease

Öz

Applications of monoclonal an tibody (mAb) technology for Virus characterisation has been an alternative approach since polyclonal antibodies have been shown to have some problemsin discriminating Viruses in conventional serological tests . MAbs to Newcastle disease virus (NDVJ have proved the considerable variation among different isola tes. Many laboratories have prepared mAbs to NDVs and shown conserved and variant epitopes on some isolates. MAbs that is spesific for all or vast majority of NDV strains and show HI actiVities are now being used in Weybridge. international referans laboratory for Newcaslle Disease (ND), for routine diagnosis. Use of these mAb's enables distinction between NDV, variant NDV. Lasota and PMV-3 Viruses. MAbs also served to group NDVs with regard to their antigenic properties. The most successful approaches on this subject were studies of Russell and Alexander. They grouped NDV strains on the hasis of their reaction with 9 mAbs to NDV Ulster 2C. The groups formed have shared im portant epidemiological properties, such as main host, virulance ete. Success of mAb grouping in epidemiology of the ND has been confirmed in many ND casesincluding ND outbreakin chickens due to variant NDVs in England and recent ND outbreakin south Marmara region of Turkey.

Kaynakça

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