Kuduz Hastalığının Teşhisinde Polimeraz Zincir Reaksiyonu

Öz

Bu çalışmada kuduz hastalığının teşhisinde Polimeraz Zincir Reaksiyonu (PCR) tekniğinin kullanım olanakların araştırılması amaçlandı . Toplanan materyallerin Floresan Antikor Tekniği (FAT) ile kontrolü sonuçları heminested RT-PCR teslinden elde edilen sonuçlar ile karşılaştırıldı. Ayrıca kokuşmuş materyallerden heminested RT-PCR ile kuduz hastalığının teşhisi olanaği araştırıIdi. Çalişmada 83 adedi FAT ile değerlendirilebifen ve 30 adedi de kokuşmuş olmaları nedeni ile FAT ile değerlendirilemeyen toplam 113 adet değişik hayvan türüne ait beyin örneği kullanıIdi. FAT ile değerlendirilebilen 83 materyalden 80 adedi kuduz yönünden müspet. 3 adedi ise kuduz yönünden menfi idi. Bu materyaller Tarım ve Köyişleri Bakanfığma bağlı kuduz teşhis lahoratuvarından temin edildi. FAT ile müspel değerlendirilen 80 materyalden 49 (% 61,25) adedinde RT-PCR ile viral nükleik asit tespit edilirken, heminested RT-PCR ile tamamında (% 1 00) viral nükleik asit saptandı. FAT ile menfi olan 3 adet materyal de RT-PCR ve heminested RT-PCR ile de viral nükleik asit tespit edilmedi. 30 adet kokuşmuş materyalden 4 adedinde RT-PCR ile viral nükleik asit (%13.33) ve 6 adedinde de heminested RT-PCR .ile viral nükleik asit (% 20) tespit edildi. Heminested RT-PCR ile viral nükleik asit tespit edilen bu 6 materyalden 3 adedi istanbul Pendik Veteriner Kontrol ve Araştırma Enstitüsünden temin edilen, diğer 3 adedi de Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsünde aşı üretimi ve deneysel çalişmalarda kullanılan hayvanlara ait beyinlerdi. Deneysel amoçlarla kullanılanlar ve kuduz virusu ile enfekte edilmiş olan hayvanlar (3 adet) hariç tutulacak olursa kokuşmuş materyallerdeki RT-PCR ve heminested RT-PCR pozitiflik oranları sırası ile % 3. 7 ( 1/2 7) ve% 11.11 (3/2 7) olarak belirlendi.

Anahtar Kelimeler

• Teşhis
• Kuduz
• RT-PCR
• Heminested RT-PCR

Polymerase C hain Reaction in the Diagnosis of Rabies

Öz

In this study the probability of the use of the PCR technique for the diagnosis of rabies was investigated. The colected specimens were examined using the FAT and the results were compared with the ones thal were obtained from the heminested RT-PCR test. Furthermore. it was investigated the diagnosis of rabies in the decomposed specimens using the heminesled RT-PCR. In this study. 83 brain specimens to evaluate using FAT and 30 decomposed materials not to evatluate using FAT belonging to various animal species totally 113 brain pecimens were used. 80 of these specimens were found to be positive using FAT while 3 of them being negative using FAT All of these specimens were obtained from the rabies diagnosis laboratories belonging to the Ministry of Agriculture and Rural Affairs. Among the 80 specimens that were found to be positive using FAT, 49 of them were determined to be positive using RT-PCR (61,25%) while all of these 80 specimens were found to be pasitive using heminesled RT-PCR (100 %). The 3 specimens that were found to be negative using FAT all of them were found to be negative using RT-PCR and heminested RT-PCR. 4 of the 30 decomposed brain specimens were determined to be positive (13,33%) us ing RT-PCR and 6 of them found to be positive using heminested RT-PCR. If the results of the brain specimens that have been used for experimental purposes and animals that have been injected with rabies virus (3 animals) are excfuded, these rates are 3. 7% and 11, 11I% respeclively in the decomposed materials. Among the 6 decomposed specimens were found to be positive using heminested RT-PCR, 3 of them were obtained. from the İstanbul Pendik Veterinary Control and Research Institute, the other 3 of them were usedfor experimental pwposes and vaccine production in Etlik Central Veterinary Control and Research Institute.

Anahtar Kelimeler

• Rabies
• RT-PCR
• Heminested RT-PCR
• Diagnosis.
• Diagnosis

Kaynakça

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