The Diagnosis of Bacterial Kidney Disease (BKD) From Cultured Rainbow Trout (Oncorhynchus Mykiss)

Öz

In this project, totally 360 samples from 5 rainbow trout farms on Kesikköprü barrage lake and aseptically 120 kidney tissues about 6-12 months rainbow trout from one rainbow trout farm from 2005 to 2007 were collected for isolation and identification of R.salmoninarum;. Ovarian (coelemic) fluids were collected from 120 mature females of fish farming on Safranbolu. KDM-2 medium was used for isolation from samples. R.salmoninarum was not isolated on totally 600 samples. DFAT and nested- PCR results of all samples were negative. As a result Bacterial Kindey Disease was not detected in Ankara and Safranbolu. In our laboratories, Bacterial Kindey Disease could be diagnosed rapidly by conventional isolation and identification with DFAT and nested-PCR on rainbow trout.

Kültürü Yapılan Gökkuşağı Alabalıklarında (Oncorhynchus Mykiss) Bakteriyel Böbrek Hastalığı (BKD)’nın teşhisi

Öz

Bu projede Renibacterium salmoninarum izolasyon ve identifikasyonu için, 2005-2007 yılları arasında, Kesikköprü Baraj Göleti’nde yer alan 5 adet alabalık işletmesinden toplam 360 adet; Safranbolu’da yer alan 1 adet alabalık işletmesinden 120 adet 6-12 aylık alabalıklardan böbrek örnekleri aseptik koşullarda alındı. Safranbolu’da yer alan 1 adet alabalık işletmesinde 120 adet olgun dişi balıklardan ovaryum sıvısı uygun koşullarda alındı. Toplanan örneklerden izolasyon için KDM-2 medium kullanıldı. Toplam 600 adet örnekten hiç R.salmoninarum izolasyonu olmadı. Aynı örneklerden DFAT ve nested-PCR testleri yapıldı. Örneklerin hiçbirinde etkene rastlanılamadı. Sonuç olarak Ankara ve Safranbolu’da Bakteriyel Böbrek Hastalığı saptanamamıştır. Laboratuarımızda alabalıklarda konvansiyonel izolasyon ve identifikasyon yanında DFAT ve nested-PCR gibi testlerle Bakteriyel Böbrek Hastalığı kısa sürede teşhis edilebilir hale gelmiştir.

Anahtar Kelimeler

• Etlik Veterinerlik Mikrobiyoloji Dergisi
• Kültür
• Gökkuşağı Balıkları
• Bakteri
• Böbrek
• Sibel ÖZKÖK
• Selahattin ŞEN
• Hikmet ÜN
• 2008

Kaynakça

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