Evcil ve Yabani Kanatlılardan İzole Edilen Newcastle Hastalığı Viruslarının Patotiplendirilmesi

Öz

Kanatlı sektöründe önemli ekonomik kayıplara neden olan Newcastle hastalığının (ND) klinik seyri virusun patojenitesi ile doğrudan ilgilidir. Velojenik suşlar önemli klinik bulgular ve yüksek mortaliteye sebep olurken, mezojenik ve lentojenik suşların meydana getirdiği hastalık nispeten daha hafif seyirli olmaktadır. Ayrıca virusun patojenitesini belirlemek epidemiyolojik açıdan da büyük önem arz etmektedir. Bu çalışmada 2007 yılında Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsünde izole ve identifiye edilen toplam 39 adet ND virusunun patojenitesi belirlendi. Virus izolasyon ve identifikasyonunda RT-PCR, Spesifik patojen free (SPF) embriyolu tavuk yumurtasına ekim, Hemaglutinasyon inhibisyon (HI) testi uygulandı. ND virusunun patotiplendirilmesinde Intracerebral Pathogenicity Index (ICPI) yöntemi kullanıldı. Köy tavuklarından izole edilen 28 ND virusunun tamamı velojenik olarak belirlendi. Evcil güvercinlerden izole edilen 4 adet ND virusunun 3’ünün mesojenik, 1’inin lentojenik olduğu tespit edildi. Yabani güvercinlerden izole edilen 5 adet NDV suşunun 2’sinin velojenik, 3’ünün ise mezojenik, yabani kumrulardan izole edilen 2 adet ND virusunun ise mezojenik olduğu tespit edildi. Yabani güvercinlerden velojenik suşların izole edilmesi ülkemizde virusun sirkulasyonunda yabani kuşların önemine dikkat çekmektedir.

Anahtar Kelimeler

• Etlik Veterinerlik Mikrobiyoloji Dergisi
• Evcil Yabani
• Kanatlı
• İzole
• Newcastle
• Patotiplendirme
• Asiye DAGMAN
• Metin GÜLEÇ
• Elçin GÜNAYDIN
• Mustafa COŞAR
• 2008

Pathotyping of the Newcastle Disease Virus Strains Isolated From the Domestic and Wild Birds

Öz

The clinical symptoms of the Newcastle disease (ND) that cause considerable losses in poultry sector are directly related to the pathogenity of the virus. When the velogenic strains cause severe clinical findings and higher mortality, disease caused by mesogenic and the lentogenic strains is relatively in a moderate mode. Also, determination of the pathogenity of the virus represents a great importance for the epidemiological meaning. In this study the pathogenity of the 39 ND viruses isolated and identified in Etlik Central Veterinary Control and Research Institute in 2007 was determined. For the isolation and identification of the virus, reverse transcription- polymerase chain reaction (RT-PCR), inoculation of the samples into the Specific Pathogen Free (SPF) embryonated eggs, and haemagglutination inhibition (HI) test were performed. The Intracerebral Pathogenity Index (ICPI) test were used for the pathotyping of the ND viruses. All of the 28 ND viruses isolated from the backyard poultry were determined as velogenic. Three of the four ND viruses isolated from the domestic pigeons were found be mesogenic, and the remaining one was lentogenic. Two of the five ND strains isolated from the wild pigeons were determined as velogenic, and three of the five were determined as mesogenic. The virus isolated from the two doves was found to be mesogenic. The isolation of the velogenic strains from the wild pigeons shows the importance of the wild birds for the circulation of the ND viruses in Tukey.

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