Klorprifos’un ratlarda ince bağırsak üzerine etkisi ve kuersetin ve kateşin’in koruyucu rolü

Öz

Bu çalışmada organofosfatlı bir pestisit olan ve yaygın olarak kullanılan klorprifos’un ratların ince bağırsakları üzerine olumsuz etkileri ve kuersetin ve kateşin’in bağırsakları koruyucu özelliği araştırıldı. Bu amaçla erkek ratlara klorprifos (5.4 mg/kg 1/25 LD50), kateşin (20 mg/kg), kuersetin (20 mg/kg), kateşin(20 mg/kg) + klorprifos (5.4 mg/kg 1/25 LD50) ve kuersetin (20 mg/kg) + klorprifos (5.4 mg/kg 1/25 LD50) kombinasyonları oral olarak dört hafta süre ile uygulandı. Uygulamadan dört hafta sonra ratların ince bağırsak dokuları histopatolojik incelemeler için alındı ve ışık mikroskobunda kontrol grubu ile kıyaslamalı olarak incelendi. Kateşin ve kuersetin uygulanan gruplar ile kontrol grubu ratların ince bağırsak dokularında histolojik olarak farklılık gözlenmedi. Klorprifos uygulanan ratların ince bağırsak dokusunda villuslarda atrofi, nekroz ve muskular mukozada ödem gözlendi. Kateşin + klorprifos ve kuersetin + klorprifos uygulanan gruplarda mikroskobik bulguların hafif şiddette olduğu gözlendi. Sonuç olarak kateşin ve kuersetinin, klorprifosun meydana getirdiği hasarı azalttığı tespit edildi.

Anahtar Kelimeler

• Yavuz ULUSOY
• Filiz DEMİR
• Fatma Gökçe UZUN
• Feriha ÖZMEN
• 2010
• Kateşin
• klorprifos
• kuersetin
• histopatoloji
• ince bağırsak.
• Etlik Veteriner Mikrobiyoloji Dergisi

Chlorpyrifos induced small intestine toxicity in rats and protective role of quersetin and catechin

Öz

In this study we investigated that chlorpyrifos which is a widely used an organophosphorous pesticide induced small intestine toxicity and protective role of quersetin and catechin. For this reason, chlorpyrifos (5.4 mg/kg 1/25 LD50), catechin (20 mg/kg), quercetin (20 mg/kg), catechin (20 mg/kg) + klorprifos (5.4 mg/kg 1/25 LD50) and quercetin (20 mg/kg) + chlorpyrifos (5.4 mg/kg 1/25 LD50) were given to male rats orally for four weeks. At the end of fourth week, histopathological changes in the small intestine tissues were investigated using light microscope comparatively with control group. At the end of the 4th week, there were no histopathological changes between the catechin-treated and quercetin-treated groups compared to the control group. After four weeks of chlorpyrifos exposure shortening and necrosis in intestinal villi and edema in muscular mucosa were observed in the small intestine tissues. Milder histopathological changes were observed in animals co-treated with catechin plus chlorpyrifos or quercetin plus chlorpyrifos. As a result, it appears that catechin and quercetin moderated chlorpyrifos-induced toxicity.

Anahtar Kelimeler

• Catechin
• chlorpyrifos
• histopathology
• quercetin
• small intestine.

Kaynakça

1. Ajay M, Achike FI, Mustafa AM, Mustafa MR, (2006). Effect of kuersetin on altered vascular reactivity in aortas isolated from streptozotocin-induced diabetic rats. Diabetes Res Clin Pract. 73, 1–7.
2. Anjaneyulu M, Chopra K, (2003). Kuersetin, a bioflavonoid, attenuates thermal hyperperalgesia in a Mouse model of diabetic neuropathic pain. Prog Neuropsychopharmacol Biol Psychiatry. 27, 1001–1005.
3. Betrosian A, Balla M, Kafiri M, Kofinas G, Marki R, Kakouri A, (1995). Multiple systems organ filure from organophosphate poisoning. J Toxicol Clin Toxicol. 33 (3), 257–260.
4. Chander V, Singh D, Chopra K, (2003). Kateşin, a natural antioxidant protects against rhabdomyolysis-induced myoglobinuric acute renal failure. Pharm Res. 48, 503–509.
5. Coşkun O, Kanter M, Korkmaz A, Oter S, (2005). Kuersetin, aflavonoid antioxidant, prevents and protects streptozocin-induced oxidative stres and β-cell damage in rat pancreas. Pharm Res. 51, 117–123.
6. Çetin A, Ulusoy Y, Öğütcü A, Uzun, FG, Demir F, (2008). Dichlorvos’un ratların ince bağırsak dokusu üzerine etkisi ve vitamin C ve vitamin E’nin koruyucu rolü. Etlik Vet Mikrobiyol Derg. 19, 47–52.
7. Goel A, Dani V, Dhawan DK, (2007). Zinc mediates normalization of hepatic drug metabolizing enzymes in klorprifos-induced toxicity. Toxicol Let. 169, 26–33.
8. Goel A, Vijayta D, Dhawan DK, (2005). Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in klorprifos-induced toxicity. Chem Biol Interact. 156, 131–140.

9. Handy RD, Abd-El Samei HA, Bayomy MFF, Mahran AM, Abdeen AM. El-Elaimy EA, (2002). Chronic diazinon exposure: pathologies of spleen, thymus, blood cells, and lymph nodes are modulated by dietary protein or lipid in the Mouse. Toxicology. 172, 13-34.
10. Kalender S, Kalender Y, Durak D, Ogutcu A, Uzunhisarcıklı M, Cevrimli BS, Yıldırım M, (2007). Methyl Parathion Induced Nephrotoxicity in Male Rats and Protective Role of Vitamins C and E. Pestic Biochem Phys. 88, 213–218.
11. Kalender S, Kalender Y, Ogutcu A, Uzunhisarcıklı M, Durak D, Acıkgöz F, (2004). Endosulfan-induced cardiotoxicity and free radical metabolism in rats: the protective effect of vitamin E. Toxicology. 202, 227-235.
12. Kalender S, Uzun FG, Durak D, Demir F, Kalender Y, (2010). Malathion-induced hepatotoxicity in rats: The effects of vitamin C and E. Food Chem Toxicol. 48, 633–638.
13. Kalender Y, Uzunhisarcikli M, Ogutcu A, Acikgoz F, Kalender S, (2006). Effects of diazinon on pseudocholinesterase activity and haematological indices in rats: the protective role of vitamin E. Environ Toxicol Pharmacol. 22, 46–51.
14. Kalender Y, Yel M, Kalender S, (2005). Doxorubicin hepatotoxicity and hepatic free radical metabolism in rats: The effects of vitamin E and kateşin. Toxicology. 209, 39–45.
15. Karaoz E, Gultekin F, Akdogan M, Oncu M, Gokcimen A, (2002). Protective role of melatonin and combination of vitamin C and vitamin E on lung toxicity induced by klorprifos-ethyl in rats. Exp Toxic Pathol. 54, 97–108.
16. Korish AA, Arafah MM, (2008). Kateşin combined with vitamisn C and E ameliorates insulin resistance (IR) and atherosclerotic changes in ages rats with chronic renal failure (CRF). Arch Gerontol Geriat. 46, 25–39.
17. Lasram MM, Annabi AB, Elj N, Selmi S, Kamoun A, El-Fazaa S, Gharbi N, (2009). Metabolic disorders of acute exposure to malathion in adult Wistar rats. J Hazard Mater. 163, 1052-1055.
18. Mansour SA, Mossa AH, (2009). Lipid peroxidation and oxidative stress in rat erythrocytes induced by klorprifos and the protective effect of zinc. Pestic Biochem Phys. 93, 34-39.
19. Morales AI, Vicente-Sanchez C, Santiago Sandoval JM, Egido J, Mayoral P, Arevalo MA, Fernandez-Tagarro M, Lopez-Novoa JM, Perez-Barriocanal F, (2006). Protective effect of kuersetin on experimental chronic cadmium nephrotoxicity in rats is based on its antioxidant properties. Food Chem Toxicol. 44, 2092–2100.
20. Ögütcü A, Ulusoy Y, Kahraman K, Uzunhisarcıklı M, Uzun FG, Taştan H, (2007). Metil Parathion’un sıçanların ince bağırsak dokusu üzerine etkisi ve vitamin C ve E’nin koruyucu rolü. Etlik Vet Mikrobiyol Derg. 18, 21–26.
21. Rezg R, Mornagui B, El-Fazaa S, Gharbi N, (2008). Caffeic acid attenuates malathion induced metabolic disruption in rat liver, involvement of acetylcholinesterase activity. Toxicology. 250, 27–31.
22. Uzun FG, Kalender S, Durak D, Demir F, Kalender Y, (2009). Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E. Food Chem Toxicol. 47, 1903–1908.
23. Uzunhisarcikli M, Kalender Y, Dirican K, Kalender S, Ogutcu A, Büyükkömürcü F, (2007). Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamins C and E. Pestic Biochem Phys. 87, 115–122.
24. Verma RS, Mehta A, Srivastava N, (2007). In vivo klorprifos oxidative stress: Attenuation by antioxidant vitamins. Pestic Biochem Phys. 88, 191–196.
25. Yu F, Wang Z, Ju B, Wang Y, Wang J, Bai D, (2008). Apoptotic effect of organophosphorus insecticide klorprifos on mouse retina in vivo via oxidative stress and protection of combination of vitamins C and E. Exp Toxicol Pathol. 59, 415–423.