Türkiye’nin Orta Karadeniz Bölgesindeki Gökkuşağı Alabalıkları (Oncorhynchus mykiss, Walbaum, 1792)’ nda Laktokokkozis ve Lactococcus Garvieae Etkeninin Antimikrobiyal Duyarlılığı

Öz

Bu çalışmada, Türkiye’nin Karadeniz Bölgesindeki gökkuşağı alabalıklarından Lactococcus garvieae izole edildi. Hastalık salgını ortalama 16.7°C su sıcaklığında Nisan-Mayıs 2009 aylarında görüldü. Hasta gökkuşağı alabalıklarında klinik olarak durgunluk, iştahsızlık, renkte koyulaşma, tek ya da çift taraflı eksoftalmus, korneada opaklaşma, gözlerde, pektoral ve anal yüzgeç tabanlarında hemoraji, karında şişkinlik, vücut yüzeyinde yaralar görüldü. Nekropside vücut boşluğunda kanlı-renkli bir sıvının varlığı, barsak ve midede sarı berrak renkte sıvı ile karakterize asites, dalak ve böbrekte büyüme, renklerinde koyulaşma, karaciğerde büyüme, renginde açılma ve peteşiyel kanamaların olduğu tespit edildi. Hastalık etkenini izole etmek için yaklaşık 50-100 g ağırlığında hasta 20 adet gökkuşağı alabalığı bakteriyolojik incelemelerde kullanıldı. Bakterilerin identifikasyonunda konvansiyonel testler kullanıldı ve hastalık etkeni L.garvieae olarak tanımlandı. İzole edilen L.garvieae suşu, amoxicillin, amoxicillin/clavulanic acid, cephalothin, chloramphenicol, doxycycline, enroflaxacin, pristinamycine ve tetracycline duyarlı olduğu tespit edildi.

Anahtar Kelimeler

• Etlik Veteriner Mikrobiyoloji Dergisi
• Lactococus Garvieae
• Fenotipik İdentifikasyon
• Antimikrobiyal Duyarlılık
• Gökkuşağı Alabalık
• Türkay ÖZTÜRK
• Behire Işıl DİDİNEN
• Gaye DOĞAN
• Ahmet ÖZER
• Recep BİRCAN
• 2013

Lactococcosis in Rainbow Trout (Oncorhynchus mykiss,Walbaum, 1792) in the Middle Black Sea Region in Turkey and Antimicrobial Susceptibility of the Aetiological Agent, Lactococcus Garvieae

Öz

In this research study, we isolated Lactococcus garvieae from rainbow trout in the middle Black Sea region in Turkey. The diseases outbreak occurred at average water temperature of 16.7°C during April-May 2009. Clinically stagnation, inapetence, darkening of the skin, exophtalmia, opacification in the cornea, hemorrhages in the eyes and at the base of pectoral and anal fins, swollen abdomen and wounds on the body surface were observed in infected fish. In necropsy; the existence of accumulation of bloody fluid in the body cavity, ascites in the intestines and the stomach characterized with a yellowish-colored liquid, enlarged and darkening in the spleen and kidney, fading in colour, existence of petechial hemorrhages and enlarged in the liver were detected. In order to isolate the aetiological agent, 20 infected rainbow trout weighting between 50-100 gr were used. As the result of conventional tests used in the identification of the bacteria, the aetiological agent was identified as L.garvieae. The isolated L.gavieae strain was determined as susceptible to amoxicillin, amoxicillin/clavulanic acid, cephalothin, chloramphenicol, doxycycline, enroflaxacin, pristinamycine and tetracycline.

Kaynakça

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