Laktasyon Periyodundaki Subklinik Mastitisli Koyunlarda Kkan ve Süt Laktoferrin Konsantrasyonlarının Araştırılması

Öz

Bu çalışmada, laktasyon periyodundaki subklinik mastitisli ve sağlıklı Karayaka ırkı koyunlarda süt ve kan serumlarında laktoferrin konsantrasyonlarının belirlenmesi amaçlandı. Araştırmada, ortalama 2-3 yaşlarında ve laktasyonlarının orta döneminde bulunan Karayaka ırkı koyunlar, Kaliforniya mastitis test ve sütte somatik hücre sayımı yöntemleri ile subklinik mastitis yönünden incelendi. Sütleri Kaliforniya mastitis test pozitif ve somatik hücre sayımı 300.000-1.000.000 olan 23 adet koyun subklinik mastitis grubunu, Kaliforniya mastitis test negatif ve somatik hücre sayımı <300.000 olan 20 adet koyun ise kontrol grubunu oluşturdu. İneklerden kan ve süt örnekleri alınarak serumları çıkarıldı. Koyunların kan ve süt serumlarında laktoferrin ölçümleri ELISA test kitleri kullanılarak gerçekleştirildi. Subklinik mastitisli ve sağlıklı koyunların serum laktoferrin değişimleri sırasıyla ile 0.90±0.08 μg/ml ve 0.85±0.08 μg/ ml olarak tespit edildi ve gruplar arasında istatistiksel olarak önemli bir fark bulunamadı (p>0.05). Süt serumu laktoferrin konsantrasyonları subklinik mastitisli ve kontrol gruplarında sırasıyla 23.98±3.45 μg/ml ve 16.84±1.29 μg/ml olarak bulundu. Subklinik mastitisli grubun süt serumu laktoferrin düzeylerinin kontrol grubununkine göre istatistiksel olarak yüksek olduğu belirlendi (p<0.01). Sonuç olarak, koyunlarda süt serumu laktoferrin konsantrasyonunun subklinik mastitisin teşhisinde faydalı bir belirteç olarak kullanılabileceği kanaatine varıldı.

Anahtar Kelimeler

• Etlik Veteriner Mikrobiyoloji Dergisi
• Koyun
• Laktasyon
• Laktoferrin
• Subklinik Mastitis
• Süt
• Cevat NİSBET
• Gül Fatma YARIM
• Gülay ÇİFTÇİ
• Nilgün GÜLTEKİN
• Sena ÇENESİZ
• Sena ÇENESİZ
• 2013

Investigation of Blood and Milk Lactoferrin Concentrations in Lactating Ewes With Subclinical Mastitis

Öz

The aim of the present study was to determine the availability of blood and milk lactoferrin concentrations in the diagnosis of subclinical mastitis in lactating Karayaka ewes. The milk samples obtained from 43 mid-lactating Karayaka ewes, aged between 2 and 3, were examined by California Mastitis Test (CMT) and somatic cell count (SCC) methods. Animals (n=23) had positive CMT score and SCC between 300.000-1.000.000 cells/ml were defined as subclinical mastitis group, whereas ewes with CMT negative and SCC between 100.000-200.000 cells/ml were described as control (healthy) group. The serum were obtained from blood and milk samples of ewes and ELISA was performed to detect blood and milk lactoferrin concentrations. The mean blood lactoferrin concentrations in subclinical mastitis group and control group were 0.90±0.08 μg/ml and 0.85±0.08 μg/ml, respectively, however the difference was not significant (p>0.05). The mean milk lactoferrin concentrations in ewes with subclinical mastitis and healthy ewes was 23.98±3.45 μg/ml and 16.84±1.29 μg/ml, respectively (p<0.01). Consequently, it is suggested that the concentration of milk lactoferrin might be a useful marker in the diagnosis of ewes with subclinical mastitis.

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