Türkiye’nin Ege Bölgesindeki gökkuşağı alabalığı (Oncorhynchus mykiss, Walbaum) anaçlarında bir hastalık etkeni Vagococcus salmoninarum

Öz

Türkiye’nin Ege Bölgesi’nde yer alan ticari bir alabalık çiftliğinde gökkuşağı alabalığı anaçlarında görülen bir hastalık esnasında ortalama %10 oranında bir ölüm kaydedildi. Hastalık belirtileri yumurtlama sonrası 10-12°C su sıcaklığında görüldü. Klinik olarak enfekte 2 anaç balıktan 6 adet bakteriyel izolat elde edildi. Bu izolatların Gr (+) kokobasil oldukları tespit edildi ve fenotipik olarak Vagococcus salmoninarum olarak teşhis edildi. İzolatlar, aynı zamanda spesifik V. salmoninarum primerleri ile PCR metodu kullanılarak teyit edildi. VG3 suşunun antibiyotik duyarlılığı Kirby-Bauer disk difüzyon metodu ile belirlendi ve amoksisillin, ampisillin, enrofloksasin, norfloksasin, oksolinik asit ve florfenikole duyarlılık gösterdi. Türkiye’de farklı coğrafik bölgelerden izole edilen V. salmoninarum izolatlarının ERIC-PCR sonucuna göre ilişkili olduğu (0.73) görüldü.

Anahtar Kelimeler

• Tansel TANRIKUL
• Meriç Lütfi AVSEVER
• Ertan Emek ONUK
• Behire Işıl DİDİNEN
• Oncorhynchus mykiss
• fenotipik
• genotipik
• karakterizasyon
• Vagococcus salmoninarum
• 2014
• Etlik Veteriner Mikrobiyoloji Dergisi

Vagococcus salmoninarum, a causative agent of disease in rainbow trout (Oncorhynchus mykiss, Walbaum) broodstocks in the aegean region of Turkey

Öz

In a commercial rainbow trout farm located in the Aegean region of Turkey, a disease outbreak with an average mortality of 10% was recorded in rainbow trout broodstocks. The disease symptoms emerged at 10-12°C water temperature after spawning periods. 6 bacterial isolates were obtained from 2 of the clinically infected rainbow trout broodstock. These isolates were observed to be Gram-positive cocobacilli and were identified phenotypically as Vagococcus salmoninarum. Isolates were also confirmed by using a PCR method with V.salmoninarum specific primers. Antibiotic susceptibility of strain VG3 was assessed by the Kirby-Bauer disk diffusion method, and the results showed that it was susceptible amoxycillin, ampicillin, enrofloxacin, norfloxacine, oxolinic acid and florfenicol. V.salmoninarum isolisolates isolated from different geographical region in Turkey were revealed to be related (0.73) as a result of ERIC-PCR. Key words: Oncorhynchus mykiss, phenotypic, genotypic, characterization, Vagococcus salmoninarum.

Anahtar Kelimeler

• Oncorhynchus mykiss
• phenotypic
• genotypic
• characterization
• Vagococcus salmoninarum

Kaynakça

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