Bandırma ve çevresinde bulunan süt sığırı işletmelerinde Listeria türlerinin varlığı

Öz

Bu çalışmada, Bandırma ve çevresinde bulunan süt sığırı işletmelerinde Listeria türlerinin varlığı araştırıldı. Bu kapsamda, Kasım 2013-Haziran 2014 döneminde, 21 adet işletmeden süt, taze dışkı, silaj, toprak, su ve süt sağım odası sıvap örnekleri olmak üzere toplam 340 adet örnek alındı. Listeria izolasyonu amacıyla süt örneklerinde AOAC/ IDF metodu, diğer örneklerde USDA/FSIS metodu kullanıldı. Çalışma sonucunda, 10 (%47.62) işletmede Listeria spp. izolasyonu gerçekleşmezken, 11 işletmede (%52.38) Listeria spp. izolasyonu yapıldı. Beş işletmenin süt, 7 işletmenin taze dışkı, 3 işletmenin silaj, 10 işletmenin toprak, 8 işletmenin su ve 2 işletmenin süt sağım odası örneklerinin en az birinden Listeria spp. izolasyonu gerçekleşti. İncelenen örneklerin %15’inden (51/340) Listeria spp. izolasyonu yapıldı. Yüz beş adet süt örneğinin 8’inden (%7.61), 105 adet taze dışkı örneğinin 12’sinden (%11.42), 21 adet silaj örneğinin 3’ünden (%14.28), 42 adet toprak örneğinin 15’inden (%35.71), 42 adet su örneğinin 11’inden (%26.19) ve 25 adet süt sağım odası örneğinin 2’sinden (%8) Listeria spp. izolasyonu gerçekleştirildi. İzolatlar kültürel ve biyokimyasal karakterlerine göre identifiye edildi ve 51 adet izolatın 8’i L. monocytogenes, 1’i L.ivanovii, 17’si L. innocua, 7’si L. seeligeri, 7’si L. welshimeri, 11’i L. grayi olarak tanımlandı. L. monocytogenes izolasyon oranı süt, taze dışkı, toprak, su ve süt sağım odası örneklerinde sırasıyla %0.95, %0.95, %4.76, %7.14 ve %4 olarak tespit edilirken silaj örneklerinden izole edilmedi. Sonuç olarak, süt sığırı işletmelerinin süt ve çevre örneklerinde Listeria türlerinin varlığı gerek hayvan sağlığı gerekse halk sağlığı açısından risk oluşturmaktadır.

Anahtar Kelimeler

• Yavuz ÇOKAL
• Listeria spp.
• Süt sığırı işletmesi
• Prevalans
• 2014
• Etlik Veteriner Mikrobiyoloji Dergisi

The presence of Listeria species in dairy cattle farms in Bandırma province, Turkey

Öz

In this study, the presence of Listeria species in dairy cattle farms was investigated. In this context, a total of 340 samples including milk, fresh feces, silage, soil, water and swabs from milking parlour were obtained from 21 dairy cattle farms between November 2013 and June 2014. For the isolation of Listeria species from samples, AOAC/IDF method in milk samples and USDA/FSIS method in other samples were used. As a result of the study, Listeria spp. was isolated from 11 dairy cattle farms (52.38%), while in other 10 dairy cattle farms (47.62%) Listeria spp. was not isolated. Isolation of Listeria spp. occurred from at least one of samples, including the milk samples from 5 dairy cattle farms, the fresh feces samples from 7 dairy cattle farms, the silage samples from 3 dairy cattle farms, the soil samples from 10 dairy cattle farms, the water samples from 8 dairy cattle farms and the milking parlour samples from 2 dairy cattle farms. Listeria spp. was isolated from 15% (51/340) of all the analyzed samples. Isolation of Listeria spp. was carried out from 8 of 105 milk samples (7.61%), 12 of 105 fresh feces samples (11.42%), 3 of 21 silage samples (14.28%), 15 of 42 soil samples (35.71%), 11 of 42 water samples (26.19%) and 2 of 25 milking parlour samples (8%). Isolates were identified by cultural and biochemical characters, and 8 of 51 isolates L. monocytogenes, 1 of 51 isolates L.ivanovii, 17 of 51 isolates L. innocua, 7 of 51 isolates L. seeligeri, 7 of 51 isolates L. welshimeri, 11 of 51 isolates L. grayi were identified. Isolation rates of L. monocytogenes were found as 0.95%, 0.95%, 4.76%, 7.14% and 4% respectively in milk, fresh feces, soil, water and milking parlour samples, however L. monocytogenes were not isolated from silage samples. Consequently, the presence of Listeria species in milk and environmental samples of dairy cattle farms pose a risk for the animal health as well as for human health.

Anahtar Kelimeler

• Listeria spp.
• Dairy cattle farm
• Prevalence

Kaynakça

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