Seroprevalence and risk factors for Toxoplasma gondii infection in shelter cats in Erzurum province of Turkey

Öz

This study was aimed to evaluate the seroprevalence and risk factors of the infection of Toxoplasma gondii in cats kept in Animal Shelter and Rehabilitation Center of Erzurum Municipality in Erzurum that collected from stray. Total 70 blood samples were collected from apparently healthy cats and diseased cats such as diarrhea, and ocular and nasal discharge. Sera samples were stored in screw-capped sterile tubes at – 20 °C until analysis. In sera samples, IgG antibodies against T. gondii were analyzed by Sabin-Feldman Dye test. Of 70 cats, 25 (35.7%) was negative and 45 (64.3%) positive for T. gondii infection. An important association was not found between young and adult, female and male, and apparently healthy and diseased cats with T. gondii seropositivity. In this study, T. gondii seroprevalence was determined at high rates in cats kept in shelter in Erzurum provinceof Turkey. The prevention measures against T. gondii infection in cats should be implemented for animal and public health concerns.

Anahtar Kelimeler

• Toxoplasma gondii
• risk factors
• seroprevalence
• cat

Türkiye’nin Erzurum ilinde barınak kedilerinde Toxoplasma gondii enfeksiyonu için seroprevalans ve risk faktörleri

Öz

Bu çalışmada Erzurum İlinde sokaktan toplanan Erzurum Büyükşehir Belediye’sine ait Hayvan Bakımevi ve Rehabilitasyon Merkezi’nde bakılan kedilerde Toxoplasma gondii enfeksiyonunun seroprevalansı ve risk faktörlerinin araştırılması amaçlandı. İshal ve göz ve burun akıntısı gibi hastalıklı kediler ve görünüşte sağlıklı kedilerden toplam 70 kan örneği toplandı. Serum örnekleri vida kapaklı steril tüplerde analize kadar -20 °C’de saklandı. Serum örneklerinde T. gondii’ye karşı IgG antikorları Sabin-Feldman Dye testi ile analiz edildi. T. gondii enfeksiyonu için 70 kedinin 25’i (%35.7) negatif ve 45’i (%64.3) pozitif idi. T. gondii seropozitifliğiyle genç ve erişkin, dişi ve erkek ve görünüşte sağlıklı ve hasta kediler arasında önemli bir ilişki belirlenmedi. Bu çalışmada Türkiye’nin Erzurum İli’nde barınakta bakılan kedilerde T. gondii seroprevalansı yüksek oranlarda belirlendi. Kedilerde T. gondii enfeksiyonuna karşı korunma önlemlerinin hayvan ve halk sağlığı sorunları bakımından uygulanması gerekmektedir.

Anahtar Kelimeler

• Toxoplasma gondii
• risk faktörleri
• seroprevalans
• kedi

Kaynakça

1. References Afonso E, Thulliez P, Pontier D, Gilot-Fromont E. (2007) Toxoplasmosis in prey species and consequences for prevalence in feral cats: not all prey species are equal. Parasitology 2007, 134(Pt.14), 1963-1971.
2. Al-Kappany YM, Rajendran C, Ferreira LR, Kwok OCH, Abu-Elwafa SA, Hilali M, Dubey JP. (2010) High prevalence of toxoplasmosis in cats from Egypt: isolation of viable Toxoplasma gondii, tissue distribution, and isolate designation. J Parasitol. 96(6), 1115-1118.
3. Awobode HO, Ohiolei JA, Adekeye TA, Adeyi AO, Anumudu CI. (2020) Shedding proportion of Toxoplasma gondii-like oocysts in feral cats and soil contamination in Oyo State, Nigeria. Parasite Epidemiol Control 11, e00181.
4. Bennett AD, Gunn-Moore DA, Brewer M, Lappin MR. (2011) Prevalence of Bartonella species, haemoplasmas and Toxoplasma gondii in cats in Scotland. J Feline Med Surg. 13(8), 553-557.
5. Bilgili A, Hanedan B. (2019) Importance of toxoplasmosis for human and animal health, present condition, problems and solution proposals in Turkey and the world. WJARR. 4, 61-74.
6. Bawm S, Phyu AZ, Chel HM, Htun LL, Nakao R, Katakura K. (2020) Seroprevalence of Toxoplasma gondii in household cats in Myanmar and molecular identification of parasites using feline faecal oocysts. Food Waterborne Parasitol. 20, e00094.
7. Boughattas S, Behnke J, Sharma A, Abu-Madi M. (2017) Seroprevalence of Toxoplasma gondii infection in feral cats in Qatar. BMC Vet Res. 13, 26.
8. Brennan A, Hawley J, Dhand N, Boland L, Beatty JA, Lappin MR, Barrs VR. (2020) Seroprevalence and risk factors for Toxoplasma gondii infection in owned domestic cats in Australia. Vector Borne Zoonotic Dis. 20 (4), 275-280.

9. Can H, Döşkaya M, Ajzenberg D, Özdemir HG, Caner A, İz SG, Döşkaya AD, Atalay E, Çetinkaya Ç, Ürgen S, Karaçalı S, Ün C, Darde M-L, Gürüz Y. (2014) Genetic characterization of Toxoplasma gondii isolates and toxoplasmosis seroprevalence in stray cats of Izmir, Turkey. PLoS One 9(8), e104930.
10. Datz C, Evason M. (2020) Toxoplasma gondii (Toxoplasmosis). In: Weese JS, Evason M. (eds). Infectious Diseases of the Dog and Cat. 1st ed. CRC Press, Taylor and Francis Group, p. 282-286.
11. Dijurkovic-Djakovic O, Dupouy-Camet J, Van der Giessen J, Dubey JP. (2019) Toxoplasmosis: overview from a One Health perspective. Food Waterborne Parasitol. 15, e00054.
12. Dubey JP. (2009) History of the discovery of the life cycle of Toxoplasma gondii. Int J Parasitol. 39, 877-882.
13. Dubey JP, Lappin MR. (2012) Chapter 79. Toxoplasmosis and neosporosis. In: Greene CE (ed.). Infectious Diseases of the Dog and Cat. 4th ed. Elsevier Saunders, USA, p. 806-821.
14. Erkılıç EE, Mor N, Babür C, Kırmızıgül AH, Beyhan YE. (2016) The seroprevalence of Toxoplasma gondii in cats from the Kars Region, Turkey. IJVM. 71, 31-35.
15. Gauss CBL, Almería S, Ortuño A, Garcia F, Dubey JP. (2003) Seroprevalence of Toxoplasma gondii antibodies in domestic cats from Barcelona, Spain. J Parasitol. 89(5), 1067-1068.
16. Gilot-Fromont E, Lélu M, Dardé ML, Richomme C, Aubert D, Afonso E., Mercier A, Gotteland C, Villena I. (2012). The life cycle of Toxoplasma gondii in the natural environment. Toxoplasmosis-Recent Advances 10, 10-34.
17. Hoffmann S, Batz MB, Morris JG Jr. (2012) Annual cost of illnes and quality-adjusted life year losses in the United States due to 14 foodborne pathogens. J Food Prot. 75, 1292-1302.
18. Jittapalapong S, Nimsupan B, Pinyopanuwat N, Chimnoi W, Kabeya H, Maruyama S. (2007) Seroprevalence of Toxoplasma gondii antibodies in stray cats and dogs in the Bangkok metropolitan area, Thailand. Vet Parasitol. 145 (1-2), 138-141.
19. Jittapalapong S, Inpankaew T, Pinyopanuwat N, Chimnoi W, Kengradomkij C, Wongnarkpet S, Maruyama S, Lekkla A, Sukthana Y. (2010) Epidemiology of Toxoplasma gondii infection of stray cats in Bangkok, Thailand. Southeast Asian J Trop Med Public Health. 41(1), 13-18.
20. Karakavuk M, Can H, Selim N, Yeşilsiraz B, Atlı E, Şahar EA, Demir F, Gül A, Özdemir HG, Alan N, Yalçın M, Özkurt O, Aras M, Çelik T, Can Ş, Döşkaya AD, Gürüz AY, Döşkaya, M. (2021) Investigation of the role of stray cats for transmission of toxoplasmosis to humans and animals living in İzmir, Turkey. J Infect Dev Ctries. 15 (1), 155-162.
21. Karakavuk M, Can H, Karakavuk T, Gül A, Alak SE, Gül C, Ün C, Gürüz AY, Döşkaya M, Döşkaya AD. (2022) Rapid detection of Toxoplasma gondii DNA in cat feces using colorimetric loop-mediated isothermal amplification (LAMP) assays targeting RE and B1 genes. Comp Immunol Microbiol Infect Dis. 81, 101745.
22. Karatepe B, Babür C, Karatepe M, Kiliç S, Dündar B. (2008) Prevalence of Toxoplasma gondii antibodies and intestinal parasites in stray cats from Niğde, Turkey. Ital JAnim Sci. 7, 113-118.
23. Katsube Y, Hagivara T, Imaizumi K. (1972) Reliability of the dye and modified hemagglutination test for the latent infection of Toxoplasma. Nihon Juigaku Zasshi. 34(3), 123-133.
24. Kokkinaki KCG, Saridomichelakis MN, Mylonakis ME, Leontides L, Xenoulis PG. (2023) Seroprevalence of and risk factors for Toxoplasma gondii Infection in cats from Greece. Animals, 13(7), 1173.
25. Lappin MR. (2014) Chapter 72: Toxoplasmosis. In: Sykes JE (ed.). Canine and Feline Infectious Diseases. 1st ed. Elsevier, Saunders, p. 693-703.
26. Matsuu A, Yokota SI, Ito K, Masatani T. (2017) Seroprevalence of Toxoplasma gondii in free-ranging and feral cats on Amami Oshima Island, Japan. J Vet Med Sci. 79(11), 1853-1856.
27. Montazeri M, Mikaeili Galeh T, Moosazadeh M, Sarvi S, Dodangeh S, Javidnia J, Sharif M, Daryani A. (2020) The global serological prevalence of Toxoplasma gondii in felids during the last five decades (1967-2017): a systematic review and meta analysis. Parasit Vectors. 13, 82.
28. Must K, Lassen B, Jokelainen P. (2015) Seroprevalence of and risk factors for Toxoplasma gondii infection in cats in Estonia. Vector Borne Zoonotic Dis. 15(10): 597-601.
29. Oi M, Yoshikawa S, Maruyama S, Nogami S. (2015) Comparison of Toxoplasma gondii seroprevalence in shelter cats and dogs during 1999-2001 and 2009-2011 in Tokyo, Japan. PLoS One 10:e0135956.
30. Özkan AT, Çelebi B, Babür C, Lucio-Forster A, Bowman DD, Lindsay DS. (2008) Investigation of anti-Toxoplasma gondii antibodies in cats of the Ankara region of Turkey using the Sabin-Feldman Dye test and an indirect fluorescent antibody test. J Parasitol. 94, 817-820.
31. Park Y, Noh J, Seo HJ, Kim KH, Min S, Yoo MS, Yun BR, Kim JH, Choi EJ, Cheon DS, Hong SJ, Yoon SS, Cho YS. (2020) Seroprevalence and B1 gene phylogeny of Toxoplasma gondii of dogs and cats in Republic of Korea. Korean J Parasitol. 58(3), 257-265.
32. Sabin AB, Feldman A. (1948) Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoon parasite (Toxoplasma). Science 108(2815), 660-663.
33. Saevik BK, Krontveit RI, Eggen KP, Malmberg N, Thoresen SI, Prestrud KW. (2015) Toxoplasma gondii seroprevalence in pet cats in Norway and risk factors for seropositivity. J Feline Med Surg. 17, 1049-1056.
34. Salman D, Pumidonming W, Oohashi E, Igarashi M. (2018) Prevalence of Toxoplasma gondii and other intestinal parasites in cats in Tokachi sub-prefecture, Japan. J Vet Med Sci. 80(6), 960-967.
35. Tagwireyi WM, Etter E, Neves L. (2019) Seroprevalence and associated risk factors of Toxoplasma gondii infection in domestic animals in southeastern South Africa. Onderstepoort J Vet Res. 86, e1-e6.
36. Tehrani-Sharif M, Jahan S, Alavi SM, Hodami M. (2015) Seroprevalence of Toxoplasma gondii antibodies of stray cats in Garmsar, Iran. J Parasit Dis. 39, 306-308.
37. Xia N, Ji N, Li L, Huang Y, Yang C, Guo X, Guo Q, Shen B, Xiao L, Feng Y. (2022) Seroprevalence and risk factors of Toxoplasma gondii in urban cats from China. BMC Vet Res. 18(1), 331.
38. Yücesan B, Babür C, Koç N, Sezen F, Kılıç S, Gürüz Y. (2019). Ankara yöresindeki kedilerde 2016 yılında Sabin-Feldman Dye testi (SFDT) ile anti-Toxoplasma gondii antikorlarının araştırılması. Turkiye Parazitol Derg. 43(1), 5.