Silybum Marianum'un Broiler Böbreğinde Methomyl Hasarına Karşı Etkilerinin Patolojik ve Biyokimyasal Olarak Araştırılması

Öz

Bu çalışmada broilerlerde Methomyl toksikasyonuna bağlı oluşan böbrek hasarına karşı Silybum marianum tohumu tozunun koruyucu/önleyici ya da iyileştirici etkilerinin araştırılması amaçlanmıştır. 28 gün olarak planlanlanan çalışmada Konrol grubu (KONT), Methomyl grubu (MET), Silybum marianum tohumu tozu grubu (SMT) ve Methomyl+Silybum marianum tohumu tozu grubu (MET+SMT) olmak üzere her birisinde 32 hayvan bulunan 4 farklı grup oluşturuldu. Methomyl 20 ppm, Silybum marianum tohumu tozu ise 10 gr/kg olarak yemlere ilave edildi. Deneme süresince haftalık olarak her bir gruptan 8 hayvanın nekropsileri yapılarak biyokimyasal ve histopatolojik incelemeler için örnekler alındı. Biyokimyasal incelemeler için alınan serum örneklerinden total protein, kreatinin ve ürik asit parametrelerin ölçümleri yapıldı. Histopatolojik incelemelerde ise böbreklerde hiperemi, tubulus epitellerinde dejenerasyon ve nekroz, Bowman kapsülünde kalınlaşma, intertubuler alanda kanama ve yangısal hücre infiltrasyonu, tubulus bazal membranlarında kalınlaşma bulguları değerlendirildi. Çalışmada her hafta kan serumlarında yapılan analizlerde kreatinin, ürik asit ve total protein değerleri KONT ve SMT gruplarında benzer bulunurken, MET gruplarında, KONT ve SMT gruplarına kıyasla istatistiksel olarak yüksek saptanmıştır. MET+SMT grubunda ise ilgili parametrelerin değerleri MET grubuna göre düşük olduğu görülmüştür. Methomyl verilen tüm gruplarda, böbrek dokularında 14’üncü gün ölçümlerde belirginleşen, 21 ve 28’inci günlerde zamana bağlı olarak şiddeti artan hasar meydana gelmiştir. Koruyucu/iyileştirici amaçla verilen Silybum marianum’un ise biyokimyasal ve histopatolojik düzeyde hasarları azalttığı gözlemlenmiştir.

Anahtar Kelimeler

• biyokimya
• böbrek
• histopatoloji
• methomyl
• silybium marianum

PATHOLOGICAL AND BIOCHEMICAL INVESTIGATION OF THE EFFECTS OF SILYBUM MARIANUM AGAINST METHOMYL DAMAGE IN BROILER KIDNEY

Öz

The aim of this study was to investigate the protective/preventive or ameliorative effects of Silybum marianum seed powder against kidney damage caused by Methomyl toxicity in broilers. In the 28-day study, 4 different groups of 32 animals each were formed as Control group (CONT), Methomyl group (MET), Silybum marianum seed powder group (SMT) and Methomyl+Silybum marianum seed powder group (MET+SMT). Methomyl 20 ppm and Silybum marianum seed powder 10 g/kg were added to the feed. During the experiment, 8 animals from each group were necropsied weekly and samples were taken for biochemical and histopathological examinations. Total protein, creatinine and uric acid parameters were measured from serum samples taken for biochemical examinations. In histopathological examinations, renal hyperemia, degeneration and necrosis of tubular epithelium, thickening of Bowman's capsule, hemorrhage and inflammatory cell infiltration in the intertubular area, and thickening of tubular basement membranes were evaluated. In the study, creatinine, uric acid and total protein values were found to be similar in the KONT and SMT groups, but statistically higher in the MET group compared to the KONT and SMT groups. In the MET+SMT group, the values of the related parameters were lower than in the MET group. In all methomyl-treated groups, damage to the kidney tissues became evident in the measurements on the 14th day and increased in severity on the 21st and 28th days in a time-dependent manner. Silybum marianum given for protective/healing purposes was observed to reduce the damage at biochemical and histopathological levels.

Anahtar Kelimeler

• Biochemistry
• Histopathology
• Kidney
• Methomyl
• Silybum marianum

Kaynakça

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