Laboratuvar farelerinin oksiyür nematodları üzerine Artemisia annua L.’nin in vivo antihelmintik etkisi

Öz

Oksiyürler, memelilerde yaygın olarak görülen bağırsak parazitleridir ve özellikle çocuklarda tekrarlayan helmintik enfeksiyonlara neden olurlar. Bu nedenle, tekrarlanabilir bir tedavi için doğal bitkisel bileşikler gereklidir. Bu çalışma, Artemisia annua L. n-hekzan ekstraktının oksiyürler üzerindeki antinematodal etkisini in vivo olarak araştırmayı amaçladı. Bu çalışmada, A. annua L. n-hekzan ekstraktı doğal olarak Syphacia obvelata ve Aspiculuris tetraptera türleri ile enfekte olmuş farelere uygulandı. 300, 600 ve 1200 mg/kg dozlarında 7 gün süreyle oral olarak verildi ve oksiyür yumurta sayısında meydana gelen değişim fekal flotasyon yöntemi ile araştırıldı. Nekropsideki nematodlar 8. günde tüm gruplarda sayı, cinsiyet ve tür açısından karşılaştırıldı. Pozitif ilaç kontrolü olarak Albendazol (ABZ) 5 mg/kg dozunda üç gün süreyle uygulandı ve çözücü kontrol olarak mısır yağı kullanıldı. Gaitada sayılan oksiyür yumurtaların aritmetik ortalaması flotasyon uygulamasının son gününde ilaç kontrol ABZ grubunda %43,51 azalma ve A. annua 1200 mg/kg grubunda %21,12 artış gösterdi. Mısır yağı grubu ile karşılaştırıldığında ABZ 5 mg/kg, A. annua 300 mg/kg ve A. annua 1200 mg/kg gruplarında nematod sayısı açısından sırasıyla %34,08, %34,76 ve %36,91 azalma saptandı. Polar olmayan A. annua L. bileşenleri, ABZ’nin çok düşük dozda olması ve çözücü solventi olarak mısır yağı kullanılması bu sonuçtan muhtemelen sorumludur.

Anahtar Kelimeler

• Antihelmintik
• Artemisia annua L.
• oksiyür
• solucan sayımı

In vivo anthelmintic effect of Artemisia annua L. on oxyurid nematodes of laboratory mice

Öz

Oxyurids are common intestinal parasites of mammals, and they are the cause of recurrent helminthic infections, especially in children Hence natural herbal compounds are necessary for a repeatable cure. This study aimed to investigate the antinematodal effect of Artemisia annua L. n-hexane extract on oxyurids in vivo. In this study, A. annua L. n-hexane extract was applied to mice naturally infected with Syphacia obvelata and Aspiculuris tetraptera species orally at 300, 600, and 1200 mg/kg doses for seven days and the change occurring in the number of oxyurid eggs was investigated by the fecal flotation method. The nematodes in the necropsy were compared on the 8th day in all groups in terms of number, gender, and species. Albendazole (ABZ) was applied at a 5 mg/kg dose for three days as positive drug control, and corn oil was used as solvent control. The arithmetic mean of oxyurid eggs counted in fecal flotation 43.51% decrease in the drug control ABZ group and showed a 21.12% increase in the A. annua 1200 mg/kg group on the last day of application. When compared with the corn oil group, a 34.08%, 34.76% and 36.91% decrease in ABZ 5 mg/kg, A. annua 300 mg/kg and A. annua 1200 mg/kg group respectively were found in their necropsies in terms of nematode number. Non-polar compounds of A. annua L., very low concentration of ABZ and as a dilution solvent corn oil are probably responsible for this result.

Anahtar Kelimeler

• Anthelmintic
• Artemisia annua L
• oxyurid
• worm counting

Kaynakça

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