Solunum Yolu Hastalığı Belirtileri Gözlenen Keçilerde Bazı Mycoplasma Türlerinin İzolasyon ve İdentifikasyonu

Öz

Keçi yetiştiriciliğinde karşılaşılan pnömoni olguları önemli verim kayıplarına yol açmaktadır. Bu nedenle solunum sistemi hastalıklarında rol oynayan bakteriyel etkenlerin izolasyon ve identifikasyonu, hastalıkla mücadelede önem arz etmektedir. Bu çalışmada makroskobik olarak pnömoni lezyonları tespit edilen keçi akciğer örneklerinde bazı Mycoplasma türlerinin izolasyon ve identifikasyonu amaçlandı. Bu doğrultuda Siirt ili Belediye mezbahasında kesimi yapılan 270 keçiden alınan akciğer örnekleri incelendi. Örneklerde Mycoplasma capricolum subsp. capricolum, Mycoplasma capricolum subsp. capripneumoniae, Mycoplasma agalactiae ve Mycoplasma putrefaciens varlığı bakteriyolojik konvansiyonel ve moleküler yöntemler kullanılarak araştırıldı. Çalışmada incelenen 270 örneğin selektif besiyerine ekim sonucunda 4 (%1.48)’ünde Mycoplasma spp. şüpheli koloniler elde edildi. Tür spesifik primerlerin kullanıldığı PCR ile örneklerin 3 (%75.0)’ünde M. capricolum subsp. capricolum, 1 (%25.0)’inde ise M. capricolum subsp. capripneumoniae identifiye edildi. Örneklerde M. agalactiae ve M. putrefaciens identifiye edilmedi. Sonuç olarak Siirt yöresinde yetiştiriciliği yapılan ve bölge halkı için önemli bir geçim kaynağı olan keçilerde meydana gelen pnömoni olgularında M. capricolum subsp. capricolum ve M. capricolum subsp. capripneumoniae etkenlerinin rol oynayabileceği belirlendi.

Anahtar Kelimeler

• Mycoplasma
• Keçi
• PCR

Isolation and Identification of Some Mycoplasma Species in Goats with Respiratory Disease Symptoms

Öz

Pneumonia cases encountered in goat breeding cause significant yield losses. Therefore, isolation and identification of bacterial agents that play a role in respiratory system diseases are important in combating the disease. In this study, it was aimed to isolate and identify some Mycoplasma species in goat lung samples with macroscopic pneumonia lesions. In this context, lung samples taken from 270 goats slaughtered in the Siirt Municipality slaughterhouse were examined. In the samples, the presence of Mycoplasma capricolum subsp. capricolum, Mycoplasma capricolum subsp. capripneumoniae, Mycoplasma agalactiae and Mycoplasma putrefaciens were investigated using bacteriological conventional and molecular methods. Mycoplasma spp. suspected colonies were obtained in 4 (1.48%) of the 270 samples examined in the study as a result of inoculation on selective medium. Using species-specific primers, PCR identified M. capricolum subsp. capricolum in 3 (75.0%) of the samples and M. capricolum subsp. capripneumoniae in 1 (25.0%) of the samples. M. agalactiae and M. putrefaciens were not identified in the samples. In conclusion, it was determined that M. capricolum subsp. capricolum and M. capricolum subsp. capripneumoniae may play a role in pneumonia cases occurring in goats, which are raised in Siirt region and are an important source of livelihood for the people of the region.

Anahtar Kelimeler

• Goat
• Mycoplasma
• Pneumoniae
• PCR

Kaynakça

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