Yenidoğan buzağı septisemilerinden elde edilen Escherichia coli izolatlarının enterotoksijenik virülans genleri, serotip ve antibiyotik duyarlılıklarının belirlenmesi

Öz

Bu çalışma, neonatal septisemili buzağılardan E. coli izolasyonu, izolatlardaki antibiyotik direncinin tespiti, sık görülen serotip ve virülens faktörlerinin belirlenmesi amacıyla yürütüldü. Bu amaçla septisemili buzağılarda PCR ile analiz edilen ve doğrulanan izolatlarda önemli serotip genleri, virülens genleri ve 8 farklı antibiyotiğe ait direnç genleri araştırıldı. Fenotipik antibiyotik direnç analizine göre izolatların % 94,7'si streptomisin, % 93,7'si tetrasiklin, % 89,5'i ampisilin, % 87,4'ü trimetoprim-sülfametoksazol, % 87,4'ü kloramfenikol, % 74,7'si enrofloksasin, % 62,1'i gentamisin ve % 24,2'si sefalotine dirençli bulundu. İzolatlarda % 88,4 tet(B), % 74,7 sul3, % 67,4 sul1, % 61 cmlA, % 56,8 qnr(B), % 54,7 tet(A), % 53,7 sul2, % 38,9 CITM, % 36,8 aadA1, % 28,4 cat1, % 26,3 qnr(S), % 12,6 aac[3]-IV, % 5,3 tet(C) ve % 1.05 qnr(A) genleri belirlendi. Serogenotipleme sonucunda izolatların % 33,7'sinde O101, % 6,3'ünde O26, % 2,1'inde ise O145 serotip genlerinin varlığı tespit edildi. Virülans genlerinin belirlenmesi amacıyla yapılan PCR çalışmaları sonucunda; İzolatların % 23.2'sinin East1, % 22.1'inin F5, % 20'sinin STa, % 17.9'unun F41 ve % 3.2'sinin LT genine sahip olduğu belirlendi. Böylece örnekleme bölgesinde neonatal septisemili buzağılarda ETEC varlığı, yaygınlığı, izolatların patotip/virotip dağılımı ortaya konuldu ve hastalığın tedavisinde kullanılabilecek en uygun antibiyotik seçeneği belirlendi.

Anahtar Kelimeler

• Antibiyotik direnci
• Buzağı
• E. coli
• Serotip
• Virülens

Yenidoğan buzağı septisemilerinden elde edilen Escherichia coli izolatlarının enterotoksijenik virülans genleri, serotip ve antibiyotik duyarlılıklarının belirlenmesi

Öz

This study was conducted for the isolation of E. coli from calves with neonatal septicemia, the determination of antibiotic resistance, common serotypes and virulence factors. For this purpose, important serotype genes, virulence genes and resistance genes of 8 different antibiotics in calves with septicemia in confirmed isolates analyzed by PCR. According to the phenotypic antibiotic resistance analysis, the isolates were 94,7% streptomycin, 93,7% tetracycline, 89,5% ampicillin, 87,4% trimethoprim-sulfamethoxazole, 87,4% chloromphenicol, 74,7% enrofloxacin, 62,1% gentamicin and 24,2% was found to be resistant to cephalothin. In isolates, 88.4% tet(B), 74.7% sul3, 67.4% sul1, 61% cmlA, 56.8% qnr(B), 54.7% tet(A), 53.7% sul2, 38.9% CITM, 36.8% aadA1, 28.4% cat1, 26.3% qnr(S), 12.6% aac[3]-IV, 5.3% tet(C) and 1.05% qnr(A) genes were identified. According to the presence of O101, O26 and O145 serotype genes, the isolates were found to be 33.7%, 6.3% and 2.1%, respectively, as a result of serogenotyping. As a result of PCR studies carried out to determine virulence genes; It was determined that 23.2% isolates had East1, 22.1% isolates F5, 20% isolates STa, 17.9% F41 and 3.2% isolates LT. Thus, the presence, prevalence of ETEC in calves with neonatal septicemia in the sampling region, the pathotype/virotype distribution of the isolates were determined and the most appropriate antibiotic option that could be used in the treatment of disease.

Anahtar Kelimeler

• Antibiotic resistance
• Calf
• E. coli
• Virulence
• Serotype

Kaynakça

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