Olgu Sunumu

Molecular detection of Eimeria stiedae in an Angora rabbit

Cilt: 26 Sayı: 2 15 Aralık 2015
  • Armağan Erdem Ütük *
  • Fatma Çiğdem Pişkin
  • İbrahim Balkaya
  • Mehmet Çağrı Karakurum
Etlik Veteriner Mikrobiyoloji Dergisi Kapak
Etlik Veteriner Mikrobiyoloji Dergisi
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Molecular detection of Eimeria stiedae in an Angora rabbit

Öz

Hepatic coccidiosis caused by Eimeria stiedae, results in severe infection, outbreaks and deaths in young rabbits. Disease is diagnosed by time consuming methods like fecal examination, oocyst sporulation and necropsy. The aim of this study was to detect E.stiedae with species specific Polymerase Chain Reaction (PCR), without waiting oocyst sporulation and necropsy. Impression smears were prepared from liver nodules and stained with giemsa. Fecal samples were collected from the intestines and examined by centrifugal flotation with salt solution to detect Eimeria spp. oocysts. Then oocysts were sporulated in 2.5% (w/v) aqueous potassium dichromate (K2Cr207). Primers specific to E.stiedae were used in PCR reaction. Eimeria spp. oocysts were detected after the examination of impression smears and bowel content. E.stiedae was diagnosed after the measurement of sporulated oocyst and PCR. At the end of the study, we think that; PCR will be very valuable for the rapid and true diagnosis of hepatic coccidiosis in rabbits.

Anahtar Kelimeler

Kaynakça

  1. 1. Bhat TK, Jithendran KP, Kurade NP, (1996). Rabbit coccidiosis and its control: A review. World Rabbit Sci. 4, 37- 41.
  2. 2. Ebtesam MM, (2008). Hepatic coccidiosis of the domestic rabbit Oryctolagus cuniculus domesticus in Saudi Arabia. World J Zool. 3, 30-35.
  3. 3. Cetindag M, Biyikoglu G, (1997). The distribution of Eimeria species in domestic rabbits of Ankara region. Acta Parasitol Turcica. 21, 301-304.
  4. 4. Erdogmus ZS, Eroksuz Y, (2006). Hepatic coccidiosis in Angora Rabbits. J Anim Vet Adv. 5, 462-463.
  5. 5. Merdivenci A, (1963). Eimeria (Coccidia) species from domestic and wild rabbits in Turkey. T Biol Derg. 13, 26-35.
  6. 6. Oncel T, Gulegen E, Senlik B, Bakirci S, (2011). Intestinal coccidiosis in Angora Rabbits (Oryctolagus cuniculus) caused by Eimeria intestinalis, Eimeria perforans and Eimeria coeciola. YYU Vet Fak Derg. 22, 27-29.
  7. 7. Tasan E, Ozer E, (1989). A study on the occurrence of Eimeria (Protozoa, Eimeriidae) in hares in the vicinities of Elazig and Tunceli. Doga Turk Vet Hay Derg. 13, 60-65.
  8. 8. Cakmak A, Vatansever Z, (2001). Coccidiosis’de tanı. In: S. Dincer (Ed.): Coccidiosis. Turkiye Parazitoloji Dernegi Yayinlari, No: 17. Meta Basim, Izmir, Turkey, p.127-132.

Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Olgu Sunumu

Yazarlar

Armağan Erdem Ütük * Bu kişi benim

Fatma Çiğdem Pişkin Bu kişi benim

İbrahim Balkaya Bu kişi benim

Mehmet Çağrı Karakurum Bu kişi benim

Yayımlanma Tarihi

15 Aralık 2015

Gönderilme Tarihi

4 Eylül 2015

Kabul Tarihi

16 Ekim 2015

Yayımlandığı Sayı

Yıl 2015 Cilt: 26 Sayı: 2

DOI

https://doi.org/10.35864/evmd.513378

IZ

https://izlik.org/JA38ZP82ET

Kaynak Göster

RIS / Bibtex
APA
Ütük, A. E., Pişkin, F. Ç., Balkaya, İ., & Karakurum, M. Ç. (2015). Molecular detection of Eimeria stiedae in an Angora rabbit. Etlik Veteriner Mikrobiyoloji Dergisi, 26(2), 41-44. https://doi.org/10.35864/evmd.513378
AMA
1.Ütük AE, Pişkin FÇ, Balkaya İ, Karakurum MÇ. Molecular detection of Eimeria stiedae in an Angora rabbit. Etlik Vet. Mik. Derg. 2015;26(2):41-44. doi:10.35864/evmd.513378
Chicago
Ütük, Armağan Erdem, Fatma Çiğdem Pişkin, İbrahim Balkaya, ve Mehmet Çağrı Karakurum. 2015. “Molecular detection of Eimeria stiedae in an Angora rabbit”. Etlik Veteriner Mikrobiyoloji Dergisi 26 (2): 41-44. https://doi.org/10.35864/evmd.513378.
EndNote
Ütük AE, Pişkin FÇ, Balkaya İ, Karakurum MÇ (01 Aralık 2015) Molecular detection of Eimeria stiedae in an Angora rabbit. Etlik Veteriner Mikrobiyoloji Dergisi 26 2 41–44.
IEEE
[1]A. E. Ütük, F. Ç. Pişkin, İ. Balkaya, ve M. Ç. Karakurum, “Molecular detection of Eimeria stiedae in an Angora rabbit”, Etlik Vet. Mik. Derg., c. 26, sy 2, ss. 41–44, Ara. 2015, doi: 10.35864/evmd.513378.
ISNAD
Ütük, Armağan Erdem - Pişkin, Fatma Çiğdem - Balkaya, İbrahim - Karakurum, Mehmet Çağrı. “Molecular detection of Eimeria stiedae in an Angora rabbit”. Etlik Veteriner Mikrobiyoloji Dergisi 26/2 (01 Aralık 2015): 41-44. https://doi.org/10.35864/evmd.513378.
JAMA
1.Ütük AE, Pişkin FÇ, Balkaya İ, Karakurum MÇ. Molecular detection of Eimeria stiedae in an Angora rabbit. Etlik Vet. Mik. Derg. 2015;26:41–44.
MLA
Ütük, Armağan Erdem, vd. “Molecular detection of Eimeria stiedae in an Angora rabbit”. Etlik Veteriner Mikrobiyoloji Dergisi, c. 26, sy 2, Aralık 2015, ss. 41-44, doi:10.35864/evmd.513378.
Vancouver
1.Armağan Erdem Ütük, Fatma Çiğdem Pişkin, İbrahim Balkaya, Mehmet Çağrı Karakurum. Molecular detection of Eimeria stiedae in an Angora rabbit. Etlik Vet. Mik. Derg. 01 Aralık 2015;26(2):41-4. doi:10.35864/evmd.513378

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