Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascarididae) larvae infecting Merlangius merlangus euxinus (Linnaeus, 1758) from the Turkish Black Sea coast based on mitochondrial small subunit ribosomal RNA gene analysis

Yıl 2019, , 64 - 69, 30.06.2019

Gökmen Zafer Pekmezci

https://doi.org/10.35864/evmd.562427

Cited By: 4

Öz

The taxonomy of Hysterothylacium
genus remains incomplete and unclear in Turkish waters. In the present study, H. adumcum larvae were morphologically identified
from Merlangius merlangus euxinus in
the Black Sea, Turkey.  The
nuclear ribosomal ITS regions (ITS-1, 5.8S subunit, ITS-2) and the small
subunit of the mitochondrial ribosomal RNA (rrnS) gene of H. aduncum were amplified and sequenced. The BLAST analysis indicated
that present ITS sequences of H. aduncum
were identical to that of the reference sequence of H. aduncum (accession no JX413596) recorded previously from the Black
Sea, Turkey. The present rrnS gene of H.
aduncum from the Black Sea, Turkey (MK886768) showed 97.94 to 99.56%
identity with isolates of H. aduncum
from the Mediterranean Sea (MF000685-MF000691) and the Chinese waters
(MF140344). Moreover, pairwise comparison between the rrnS sequences of the H. aduncum from the Black Sea, Turkey (MK886768)
and others H. aduncum isolates from
the Mediterranean Sea (MF000685-MF000691), the Chinese waters (MF140344) showed
differences ranged from 0.2 and 1.7%.  With
the present study, H. aduncum from the Black Sea was characterized for
the first time by sequencing of the mitochondrial rrnS gene.

Anahtar Kelimeler

Hysterothylacium aduncum, Molecular characterization, mitochondrial rrnS gene, Black Sea, Turkey

Küçük alt ünite ribozomal RNA gen analizine dayanarak Türkiye’nin Karadeniz kıyılarından Merlangius merlangus euxinus’u (Linnaeus, 1758) enfekte eden Hysterothylacium aduncum (Nematoda: Raphidascarididae) larvasının moleküler karakterizasyonu

Öz

Türkiye
sularında Hysterothylacium cinsinin
taksonomisi eksik ve belirsizdir. Bu araştırmada Türkiye’nin Karadeniz
kıyılarında avlanan Merlangius merlangus
euxinus’da H. aduncum larvaları
morfolojik olarak teşhis edildi. H.
aduncum’un nükleer ribozomal ITS gen bölgesi (ITS-1, 5.8S subunit, ITS-2) ile
küçük alt ünite mitokondriyal ribozomal RNA (rrnS) gen bölgesi çoğaltıldı ve
sekanslandı. BLAST analizleri H. aduncum’un mevcut ITS dizilerilerinin
daha önce Türkiye Karadeniz’den kayıt edilen H. aduncum’un referans dizisiyle (erişim numarası JX413596) aynı
olduğunu gösterdi. Türkiye Karadeniz’den H.
aduncum türünün rrnS geni (MK886768), H.
aduncum'un Akdeniz (MF000685-MF000691) ile Çin sularındaki (MF140344) izolatları
ile %97.94-99.56 arasında benzerlik gösterdi. Bununla birlikte, Türkiye Karadeniz’den
elde edilen H. aduncum’un rrnS dizisi
ile H. aduncum'un Akdeniz
(MF000685-MF000691) ile Çin sularındaki (MF140344) diğer izolatlarının ikili
karşılaştırmasında %0,2 ile %1,7 arasında farklılık gözlendi. Bu çalışma ile ilk
kez Karadeniz'den H. aduncum’un mitokondriyal
rrnS geni moleküler olarak karakterize edildi.

Anahtar Kelimeler

Hysterothylacium aduncum, Moleküler Karakterizasyon, Mitokondriyal rrnS geni, Karadeniz, Türkiye

Kaynakça

• Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, (1990). Basic local alignment search tool. J Mol Biol. 215, 403-410.
• Amor N, Farjallah S, Merella P, Said K, Ben Slimane B, (2011). Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascaridae) from different fish caught off the Tunisian coast based on nuclear ribosomal DNA sequences. Parasitol Res. 109, 1429-1437.
• Berland B, (1961). Nematodes from some Norwegian marine fishes. Sarsia 2, 1-50.
• Berland B, (1991). Hysterothylacium aduncum (Nematoda) in fish. ICES. 44, 1-4.
• Costa A, Graci S, Cammilleri G, Buscemi MD, Collura R, Vella A, Ferrantelli V, (2018). Molecular identification of Hysterothylacium spp. in fishes from the Southern Mediterranean Sea (Southern Italy). J Parasitol. 104(4), 398-406.
• D’Amelio S, Barros NB, Ingrosso S, Fauquier DA, Russo R, Paggi L, (2007). Genetic characterization of members of the genus Contracaecum (Nematoda: Anisakidae) from fish-eating birds from west-central Florida, USA, with evidence of new species. Parasitology 134, 1041-1051.
• Felsenstein J, (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783-791.
• Gazzonis AL, Cavallero S, Zanzani SA, Olivieri E, Malandra R, Ranghieri R, D’ Amelio S, Manfredi MF, (2017). Anisakis sp. and Hysterothylacium sp. larvae in anchovies (Engraulis encrasicolus) and chub mackerel (Scomber colias) in the Mediterranean Sea: Molecular identification and risk factors. Food Cont. 80, 366-373.
• Guo YN, Xu Z, Zhang LP, Hu YH, Li L, (2014). Occurrence of Hysterothylacium and Anisakis nematodes (Ascaridida: Ascaridoidea) in the tanaka’s snailfish Liparis tanakae (Gilbert & Burke) (Scorpaeniformes: Liparidae). Parasitol Res. 113(4), 1289-1300.
• Hillis DM, Bull JJ, (1993). An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol. 42, 182-192.
• Keskin E, Koyuncu CE, Genc E, (2015). Molecular identification of Hysterothylacium aduncum specimens isolated from commercially important fish species of Eastern Mediterranean Sea using mtDNA cox1 and ITS rDNA gene sequences. Parasitol Int. 64(2), 222-228.
• Klimpel S, Kleinertz S, Hanel R, Rückert S, (2007). Genetic variability in Hysterothylacium aduncum, a raphidascarid nematode isolated from sprat (Sprattus sprattus) of different geographical areas of the northeastern Atlantic. Parasitol Res. 101, 1425-1430.
• Kumar S, Stecher G, Tamura K, (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 33, 1870-1874.
• Li L, Zhang LP, Liu YY, (2013). Hysterothylacium simile n. sp. (Nematoda: Raphidascarididae) and H. aduncum (Rudolphi, 1802) from marine fishes in the Bohai and Yellow Sea, China, with comments on the record of H. paralichthydis (Yamaguti, 1941) in the Chinese waters. Syst Parasitol. 84, 57-69.
• Li L, Lü L, Nadler SA, Gibson DI, Zhang LP, Chen HX, Zhao WT, Guo YN, (2018). Molecular phylogeny and dating reveal a terrestrial origin in the early carboniferous for ascaridoid nematodes. Syst. Biol. 67(5), 880-900.
• Moravec F, Nagasawa K, Urawa S, (1985). Some fish nematodes from fresh water in Hokkaido, Japan. Folia Parasitol. 32, 305-316.
• Moravec, F, Justine JL, (2015). Anisakid nematodes (Nematoda: Anisakidae) from the marine fishes Plectropomus laevis Lacépède (Serranidae) and Sphyraena qenie Klunzinger (Sphyraenidae) off New Caledonia, including two new species of Hysterothylacium Ward & Magath, 1917. Syst Parasitol. 92(3), 181-195.
• Pekmezci GZ, Bolukbas CS, Gurler AT, Onuk EE, (2013). Occurrence and molecular characterization of Hysterothylacium aduncum (Nematoda: Anisakidae) from Merlangius merlangus euxinus and Trachurus trachurus off the Turkish coast of Black Sea. Parasitol Res. 112, 1031–1037.
• Pekmezci GZ, Onuk EE, Bolukbas CS, Yardimci B, Gurler AT, Acici M, Umur S, (2014a). Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. Vet Parasitol. 201, 82–94.
• Pekmezci GZ, Yardimci B, Onuk EE, Umur S, (2014b). Molecular characterization of Hysterothylacium fabri (Nematoda: Anisakidae) from Zeus faber (Pisces: Zeidae) caught off the Mediterranean coasts of Turkey based on nuclear ribosomal and mitochondrial DNA sequences. Parasitol Int. 63, 127-131.
• Pekmezci GZ, Umur S, (2015). Presence of Hysterothylacium gadi aduncum (Rudolphi, 1802) (Anisakidae) in cultured Rainbow trout (Oncorhynchus mykiss Walbaum, 1792) in freshwater farms from Turkey and its mortality. Iran J Fish Sci. 14(1), 150-157.
• Pekmezci GZ, Yardimci B, (2019). On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters. Parasitol Res. 118(5), 1393-1402.
• Peter AJ, Maillard C, (1988). Larves d’ascarides parasites de poisons en Mediterranee occidentale. Bull Mus Natl Hist Nat. 10 (sect. A), 347-369.
• Simsek E, Ciloglu A, Aypak S, Yildirim A, Aldemir OS, Unlu AH, Pekmezci GZ, (2016). First molecular characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) caught off the Aegean Region streams, Turkey. Kafkas Univ Vet Fak Derg. 22 (4), 529-532.
• Simsek E, Ciloglu A, Yildirim A, Pekmezci GZ, (2018). Identification and molecular characterization of Hysterothylacium (Nematoda: Raphidascarididae) larvae in bogue (Boops boops L.) from the Aegean Sea, Turkey. Kafkas Univ Vet Fak Derg 24, 525-530.
• Tedesco P, Gustinelli A, Caffara M, Patarnello P, Terlizzi A, Fioravanti ML, (2018). Hysterothylacium fabri (Nematoda: Raphidascarididae) in Mullus surmuletus (Perciformes: Mullidae) and Uranoscopus scaber (Perciformes: Uranoscopidae) from the Mediterranean. J. Parasitol. 104(3), 262-275.
• Thompson JD, Higgins DG, Gibson TJ, (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.
• Zhu X, Gasser RB, Podolska M, Chilton NB, (1998). Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int J Parasitol. 28, 1911-1921.