Studies on the preparation and antigenicity of the vaccine against leptospirosis.

Öz

The vaccine was prepared fröm Leptospira grippotyphosa Haymana strain which was isolated by the author from Haymana- Ankara. The culture was grawn in Chang's medium supplemented with vitamin Bıı for 9 days. Formelin (37 %) was added in the ratio of 0,05 % after harvesting the culture. The number of the leptospira organisms were counted with a haemocytometer and the culture was filtered through types SM, 11305-0,65 pore size sartari us membrane. Leptospira organisms on the filter membrane we.re collected in to a steril flask at concentration of 1,5 x 109 /ml. with saline solution containing 0,01 % merthiolate·. The monovalent vaccine was produced from Haymana-strain. CL. grippotyphosa) purity of the vaccine was ascertained in nutrient broth, blood agar (aerobic and anaerobic). Fletcher's agar, Chang medium, Littman Oxgall agar, mycobiotic agar and Sabourod's medium. Safety of the vaccine was checkedin goats, rabbits, guinea pigs and hamster.s InnocuÜy of the vaccine was alsa checked by inoculating two guinea pigs with 5 ml. of the vaccine. For untowards reaction vaccinated animals were obserwed for a period of 21 days. At 21 post vaccination day blood samples from the tes animals were tested by micro agglutination Lysis test. and titres were as follows: Two goats 1/100, one· rabbit 1/200, two 1/800, two 1/1600 and guineo-pigs 1/100- 1/800. The efficeacy of the vaccine w as tes te d by chaJlenging. vaccinated and 12 control hamsters at 21 post vaccination day with a virulent strain of L. grippotyphosa. All of the control hamsters died within 14 days wheras the vaccinated animals survived.

Anahtar Kelimeler

• Studies
• preparation
• antigenicity

Leptospirosis'e Karşı Etkin Bir Aşı Hazırlanması ilee Bu Aşının Keçi, Tavşan, Kobay Ve Hamsterlerde Bağışıklık Denemeleri

Öz

Bu çalışma Ülkemizde u~un zamandır eksikliği duyulan leptospirosis hastalığına karşı bir aşı hazırlamak için yapıldı. Aşımızda Ankara- Haymadan izole ettiğimiz, patojen L. grippotyphosa sero grubundan yerel haymana suşu kullanıldı. Aşı üretimi Bıı vitamini katılmış Chang vasatında yapıldı. Üretimden sonra % 0,05 formone inaktive edildi. Aşı süzüldükten sonra 1,5 x 10-ıı germ olacak şekilde % 0,01 lik merthioletli fizyolojik tuzlu su ile sulandırıldı. Aşı mono:.. valan, formolle· inaktive edilmiş bir aşıdır. Sterilite kontrolunda aşınan saf ve temiz oJduğu saptandı. Bağışıklık testlerinden M.A testi ile keçi, tavşan, kabaylatın kan serumları işlendi. Yeterli seviyede immun antikdrlar bulundu. Aynı hayvanlarda aktif protektif test uygulandı. % 69 -% 84,5 arasında bağışıklık sağlandı.

Anahtar Kelimeler

• Leptospirosis
• Leptospirosis'e karşı Aşı
• Leptospirosis Aşısı
• Keçi
• Tavşan
• Kobay
• Hamster
• A. Altan BULU
• 1979
• 1980
• 1981

Kaynakça

1. 1 - Anderson, L. E., Jenkin H. M., 1972. Estimation of leptospiral viabitity with agar pour plates. Am. J . Vet. Res., 33 (12) , 2487-2592.
2. 2 - Babudieri, B., Bussinello, E., Bajocchi, E., Salvi, A., Massa, L., 1955. Studi sulla lestospirosi delle· riasie della valle padana Ricerche epidemiologiche profila.ssi vachinale. Riv. Infort .. 42, 453.
3. 3 - Ba budieri, B., Leptospira aşı protokolu.
4. 4 - Bey, R. F., Johnson, R. C., 1978. Hummoral immun respanses of cattle vaccinated with leptospiral pentavalent outer envelope and whole culture vaccine Am. I. Vet. Res., 39 (7) , 1109-1114.
5. 5 - Bey, R. F., Johnson, R. C., 1978. Humoral immune response of dogs vaccinated wLth leptospiral pentavalent outer enevlope and whole culture vaccines. Am. J . Vet. Res., 39 (5) , 831-836.
6. 6 - Cacchione, R. A., Saravi M. A., Cascell, E. S., Martinez, E. S., 1977. Trivalent vaccine against canine leptospirosis I. serological respanses and laboratory tests) Revista de medicina veterinaria, Argentina 58 (5 / 6) 419-422, 425 Reff. : Vet. Rull. 1979. 316-2433.
7. 7 - Finn, M. A., Jenkin H. M., 1972. Gytopathic effects of · leptospim serotypes patoc and canicola in threekidney cell culture. systems. Am. J. Vet. Res., 34 (5), 669-672.
8. 8 - Fish, N. A., Kings Cote, B., 1973. Pro.tection of gilts leptospirosis by use of a li ve vaccine. Canadian Veterinary Journal 14 (1), 12-15.

9. 9 - Hanson, L. E., Tripathy, D. N., Killinger, A. H., 1972. Current Status of leptospirosis Lmmunization ins Swine · and cattle journal of the American Veterinaıry Medical Association 161 (ll), 1235-1243.
10. 10 - Jiran, E., Kadlec, V., Svatos, L., 1972. A freeze-dried trivalent vaccine against canine leptospirosis Veterinarni Medicina. 17 (6), 367-377. Reff. : Vet. Bull. 1973, 7, 69.
11. ll - Kida, H., Ishıı, T., Tsunehisa, Y., Yamamoto, S., Yanagava, R., 1977. Comparison of vaccines prepared from leptospires grown in a serum medium and in a chemically defined medium. Zentralblatt pür Bak-'teriologie parasitenkunde, infektions krank heiton and Hgiene Erste Abteilung originale 238 A (.1) 86-96
12. 12 - Morris, J. A., . Orr, H. S., 1979. Immunity induced hamters vaccinated with a ribosome extract of leptospira inter organs seragrup icterohaemorrhagiae. J o u mal of Biological Standardization 7 (1), 81-87 U. K.
13. 13 - Nowakowski, J., Orzechowska-Kulawczuk, M., 1977. Immunogenicity of vaccines against. Leptospirosis in dogs Mdycyna Weterynaryjina 33 (9) , 524-526.
14. 14 - Nowakowski, J., Orzechowska-Kulawczuk M., Majdan, S. 1977. Agglutinin and protective antibodies in pigs vaccineted 'with monovalent vaccines against leptospirosis. Medycyna Weterynarjna 33 (8}, 455-458.
15. 15 - Petrov, V. F., Bezborodkin N. S., 1972. Dynamics of formation of immuniıty to leptospirosis in pigs inoculated with combined vaccine against swine fever. Erysipelas and leptospi:rosis 25, 7-10 (Ru) . Vet. Inst. uı. Lenina. Vitebsk Belorusskaya SSR. Reff. : Vet. Bull. 1973. 216-2487.
16. 16 - Riademann, S., Zamora, I ., 1977. Bovine amniotic fluid as a substitute for rabbit serum in the culture of leptospi·rosis. Revista Latin Arnericana de microbilogia 19 (2), 83-86 Chile. Ref. : Vet. Bull. 1979. 401. 3090.