Koçlarda Aglutinasyon ve Agar- Jel Diffuzyon Yöntemiyle Brucella Ovis Antikorlarının Saptanması Üzerinde Araştırmalar

Öz

Bu araştırma.da kullanılan 205 koç serumundan 185'i Et ve Balık Kurumu'na, Konya Cihanbeyli kazasından kesime gelen hayvanlardan, 15'i Enstitümüzde deneme hayvanı olarak kullanılan koçlardan ve 5'i de Adana Mercimek Harasından Lôboratuvarımıza muayene için gelen koç serumlarından sağlanmıştır. Bu serumlar aynı zamanda serum aglutinasyon ve agar jel diffuzyon test yöntemiyle Bruceila ovis'e karşı yoklanmış olup serum aglutinasyon testinde 166 serum 1/10-1/640 ara.sında değişen aglutinin titreleri göstermiştir. Bu testte 43 serum pozitif, 6 şüpheli ve 156 serum negatif reaksiyon verirken, Agar jel diffuzyon testinde 50 serum pozitif, 155 serum da negatif sonuç vermiştir. Aglutinasyon testinde şüpheli 5 serum negatif, 3 serum agar jel diffuzyon testinde pozitif reaksiyon vermiştir. Yine agar jel'de negatif olan bir serum aglutinasyonla pozitif sonuç vermiştir. 42 serum iki testte de parelel çalışmış olup uyum yüzde % 84 dür. Buna. göre agar jel diffuzyon testinin daha kesin sonuç vermesi, bu testin duyarlı, basit ve ekonomik oluşuyla serolajik kanıda önemli bir yeri olduğu kanısına varılmıştır.

Anahtar Kelimeler

• Koç,Brucella Ovis,Brucella Ovis Antikor Saptanması,Mübeccel YUMUŞAK,1984

Studies on aglutination and agar- gel diffusion test for the detection of antibodies against Brucella ovis in rams.

Öz

This study ıwas carried out using 205 blood serum of ram collected mainly from Konya, Ankara and Adana proices of the country. All serums ware examined both by serum aglutination and agar-gel diffusion tests comparisonly Br. ovis By serum aglutination tests 166 serums showed 1/10-1/640 agglutinin titres among them 43 serum !were positives, 156 negatif and 6 suspicion reactions were obtained. By agar gel diffusion test, 50 serum gave positive reactions and 155 ıwere negatives from 5 serums of suspicion and from 3 negatives reactions by agglutination tests were positives by agar gel diffussion tests on the otherland one serum sample showed negative reactions by agar gel diffuion test ıwas positive by agglutination test 42 (84) of the pasitives serums ıwere corresponded by both tıwo tests used. it seemed to be a.gar gel diffusion tests more sensitive simplier and economic, a.nd more valid than aglutinin tests for Bruceila ovis.

Kaynakça

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