«Studies on isolation of Haemophilus somnus from bovine diseased and normal pulmonary tissues»

Öz

Haemophilus somnus was isolated and identified from four lungs of 130 lungs with pneumonia and from one of 90 lungs with normal appearance. The collected materials were seeded on to blood agar, Mac Conkey and brain-heart infusion agar with blood and yeast extract using steril swabs. The organisms grew well only on brain-heart infusion agar with blood and yeast extract in the presence of % 1 O C02 atmesphere and after 48 hours incubation. The colonies were greyish yellow in colour, translucent, rounded, 1 mm in diamater and butter-like in consistency. The isolated organisms were gram negative, pleomophic and coccobacilli and they fermented glucose. lndol and oxidase tests were positive but catalase negative. All the isolates tested with H.somnus antiserum and gave agglutination in the slide-agglutination test.

Normal ve Pnöymonili Sığır Akciğerlerinden Haemophilus Somnus İzolasyonu Üzerinde Çalışmalar

Öz

130 Pnöymonili akciğerden dört ve 90 normal görünüşlü sığır akciğerinden 1 adet Haemophilus somnus izole ve idenlifiye edildi. Toplanan materyallerden steril eküvyonlarla kanlı agar, Mac Conkey agar ve kanlı - maya özetli beyin-kalp infüzyon agara ekimler yapıldı. Sadece kanlı-maya özetli beyin-kalp infüzyon agarda% 10 CO2 atmosferde, 48 saat inkubasyondan ·sonra üreme belirlendi. Koloniler; grimsi sarı renkli, tereyağ kıvamında, 1 mm çaplı, yarı saydam, yuvarlak şekilli olarak görüldüler. Izole edilen gram negatif, pleomorfik, küçük kokobasil şeklindeki mikroorganizmalar glukozu fermente ettiler. lndol ve oksidaz pozitif, katalaz negatif bulundu. H.somnus antiserumu ile kontrol edilen izolatların tümü lamda aglutinasyon verdi.

Anahtar Kelimeler

• Sığır
• Sığır Akciğerlerinden Haemophilus Somnus İsolazyonu
• Haemophilus Somnus İsolazyonu
• 1992
• Vildan ÖZDEMİR
• Etlik Veteriner Mikrobiyoloji Dergisi

Kaynakça

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