The Diagnosis of Bovine Brucellosis With Serum Agglutination, Complement Fixation and İmmunacob Tests

Öz

In this study, the use, efficacy and reliability of the commerical ImmunoCom b test kit, which works according to the enzyme immunoassay principle, together with other serological tests such as SAT and CFT in the detection of specific antibodies against bovine brucellesis were investigated. A total of 68 bovine sera obtained from brucellesis suspected as well as aborted cattle were examined with SAT,CFT and IC.38 (56%), 28 (41 %) and 38 (56%) sera were proved to be positive, respectively. Therefore, IC=SAT>CFT . result was obtained in the detection of specific antibodies. As a result, the use of İC, which is a sensitive, practical and rapid method, together with other conventional tests working with different antibody groups (IgM,IgG) such as SAT and CFT, was proved to be beneficial in the diagnosis of the infection.

Sığır Brucellosis'inin Serum Aglutinasyon, Komplement Fikzasyon ve İmmunocomb Testleriyle Teşhisi

Öz

Bu çalışmada, sığırlarda brucella infeksiyonuna karşı oluşan spesifik antikodann ortaya konulmasında rutin olarak kullanılan Serum Aglutinasyon (SAT) ve Komplement Fikzasyon ( CFT) testlerinin yanısıra enzim immunoassay prensibi ile çalışan ticari immunocomb test (IC) kitinin rutin uygulamaya dahil edilip edilerniyeceği araştırıldı. Abort yapmış veya bruceila şüpheli 68 sığırın kan serumlan SAT, CFT ve lC testleri ile değerlendirildi . Sırasıyla, 38 (%56), 28 (%41) ve 38 (%56) pozitif serum belirlendi. Buna göre, pozitifleri belirlemede IC=SAT>CFT sırası elde edildi. Sığır brucellosis 'nin serolojik teşhisinde, değişik antikor gruplan (lgM,IgG) ile çalışan konvansiyonel testler (SAT,CFT,vs) yanısıra immunocomb gibi duyarlı, pratik, çabuk ve saha koşullannda da yararlanılabilen bir testin de kullanılmasının, infeksiyenun teşhisinde yararlı olacağı sonucuna vanldı.

Anahtar Kelimeler

• Sığır
• Brucellos
• Serum
• Aglutinasyon
• Komplement Fiksasyon
• İmmunocomb
• Sığır Brucellosis'in Teşhisi
• Hakan YARDIMCI
• Ömer ESENDAL
• Nejat AYDIN
• 1996
• Etlik Veteriner Mikrobiyoloji Dergisi
• cft

Kaynakça

1. Alton, G.G.,Jones,L.M. and Pietz,D.E.(1975): Laboratoıytechniques in brucellosis. 2th ed., World Health Organization, Monograph Series, No.55,Geneva.
2. ANON ( 1986) : Joint FAO/WHO expert commitee on brucellosis. WHO/ Technical Report, 6th Report, Series No.740, Genova.
3. ANON (1994): Kanatlı ve çiftlik hayvanlannın bazı hastalıklarında pratik teşhis metodlanyla ilgili yeni ürünler. Pethast AP., Biogal (Galed Lab.), İsrail.
4. ANON (1994): ImmunoComb-Sığır brucella antikor test kiti kullanma talimatlan. Pethast AP., Biogal (Galed Lab.), İsrail.
5. Arda,M.,Minbay,A ve Aydın,N. (1982): Özel mikrobiyoloji, bakteriyel infeksiyöz hastalıklar. AÜ.Basımevi,AÜ.Vet.Fak.Yay. , No.386, Ders Kitabı : . 284, Ankara.
6. Aydın,N. ,Bisping,W., Akay, Ö. und Kirpal, G.(1988): Untersuchungen zum vorkommen boviner brucellose in der Turkei und bewertung der immunnisierendern wirkung zweier vakzinen. Beri. Münch. Tierarztl. Wschr.,101: 109-113.
7. Byrd, J.W.,Heck,F.C. and Hidalgo, R.J.(1979): Evaluation of the enzyme linked immunosorbent assay for detecting Brucella abortus antibodies. Am.J.Vet.Res.,40(6): 896-898.
8. Cargili,C.,Lee,K.and Clarke, !.(1985): Use of an enzyme-linked immunosorbent assay in a bovine brucellosis eradication program. Aust.Vet.J.,62(2):49-51.

9. Chappel,R.,McNaught,D.J.,Bourke,J.A and Allan,G.S.(l978): The diagnostic efficiency of some serological tests for bovine brucellosis. J.Hyg.Camb.,80:373-383 .
10. Corbel,M.J.anda Brinley-Morgan,W.J.(1984): Genus brucella Meyer and Shaw 1920,173AL in: Krieg, N.R.:Bergey's Manual ofSystematic Bacteriology. Baltimore/London,Williams and Wilkins, Volume 1, pp.377-378 .
11. Dohoo,I.R.,Wright,P.F.,Ruckerbauer,G.M.Samagh,B,S:,Robertson, . F.j .and Forbes,L.B.(1986) : A comparison of five serological tests for bovine brucellosis. Can. Vet.Res.,50:485-433.
12. Heck,F.C.Williaıns,J.D.,Crawford,R.P. and Flowers,A.I.: Comparison of serological methods for the detection of B.abortus antibodies in sera from vaccinated and non-vaccinated cattle.J .Hyg.Caınb.,83:491-499.
13. Nicoletti,P.(l980): The epidemiology of bovine brucellosis. Adv.Vet.Sci.Comp.Path.,24:69-97.
14. Rivetz,B.,Weisman,Y,Fish,F.and Herzberg,M.(l985): Evaluation of a novel rapid kit for the visual detection of newcastle disease virus antibodies. Avian Dis.,29( 4):929-942.
15. Sutherland, S.S .(I980): Immunology of bovine brucellosis . Vet.Bull.,50:359-368.
16. Tizard,I.(l983): An introduction to veterinary immunology.2th ed., W. B. Saunders Co.,Philadelphia.
17. Voller,A.,Bidwell,D.E. and Bartlett,A.(l979): The enzyme linked immunosorbent assay (ELISA).Dynatech Europa Borough.House, London.