The assessment of the protein profiles and oxidant/antioxidat status in conjunctival Brucella melitensis Rev1 vaccinated sheep

Öz

Brucellosis is a zoonotic and economically significant animal disease worldwide. The most frequently used vaccine to avoid brucellosis in small ruminants is the Rev1 conjunctival Brucella melitensis vaccine. The aim of this study was to investigate the effects of B.melitensis Revl conjunctival vaccine on total protein, albumin, globulin levels, protein profiles and oxidant/antioxidant status in sheep. Ten sheep were used as animal material for this purpose. The bloods taken before vaccination were used as negative control. The sera obtained one month after administration of single dose B.melitensis vaccine were used as experimental materials. The spectrophotometric method estimated total protein, albumin, globulin levels, total antioxidant capacity (TAS), and total oxidant capacity (TOS). Protein profile was determined by sodium-dodecyl-sulphate and native-polyacrylamide gel electrophoresis methods. It was determined that total protein and globulin levels increased slightly in sero positive sheep (P>0.05). There was no difference for protein profiles in both electrophoresis methods. The band densities of albumin decreased but gamma globulin increased slightly after vaccination. TAS levels decreased significantly (P<0.05), but TOS levels increased slightly (P>0.05). In conclusion, Rev-1 conjunctival Brucella vaccine was thought to be safe to use to prevent Brucellosis, and the addition of antioxidant after vaccination can reduce oxidative stress.

Anahtar Kelimeler

• Brucella melitensis Rev1
• Conjunctival vaccine
• Oxidative stress
• Protein profile.

Konjunktival Brucella melitensis Rev1 ile aşılanmış koyunlarda protein profillerinin ve oksidan/antioksidan durumunun değerlendirilmesi

Öz

Bruselloz, dünya çapında zoonotik ve ekonomik açıdan önemli bir hayvan hastalığıdır. Küçükbaş hayvanlarda brusellozdan korunmak için en sık kullanılan aşı Rev1 konjunktival Brucella melitensis aşısıdır. Bu çalışmanın amacı, koyunlarda B. melitensis Revl konjunktival aşısının total protein, albümin, globulin düzeyleri, protein profilleri ve oksidan/antioksidan durumuna etkilerini araştırmaktır. Bu amaçla hayvan materyali olarak on koyun kullanıldı. Aşılama öncesi alınan kanlar negatif kontrol olarak kullanıldı. Tek doz B. melitensis aşısının uygulanmasından bir ay sonra elde edilen serumlar deney materyali olarak kullanıldı. Total protein, albümin, globulin seviyeleri ile antioksidan kapasite (TAS) ve oksidan kapasiteyi (TOS) spektrofotometrik yöntem ile belirlendi. Protein profili, sodyum-dodesil-sülfat ve natif-poliakrilamid jel elektroforez yöntemleri ile belirlendi. Sero-pozitif koyunlarda total protein ve globulin düzeylerinin hafif yükseldiği belirlendi (P> 0.05). Her iki elektroforez yönteminde de protein profilleri açısından fark bulunmadı. Aşılamadan sonra albüminin bant yoğunluklarının azaldığı, ancak gama-globülin yoğunluklarının hafifçe artmış olduğu tespit edildi. TAS düzeyleri önemli ölçüde azalmışken (P <0.05), TOS düzeylerinde biraz artış gözlendi (P> 0.05). Sonuç olarak, Rev-1 konjunktival Brucella aşısının Brucellozdan korunmak için kullanımının güvenli olduğu ve aşılamadan sonra rasyona antioksidan eklenmesinin oksidatif stresi azaltabileceği düşünüldü.

Anahtar Kelimeler

• Brucella melitensis Rev1
• Konjunktival aşı
• Oksidatif stres
• Protein profili.

Kaynakça

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