Microfluidic catalase biosensor designed for efficacy of hydrogenperoxide decontamination: Microbial optimization

Öz

Bio-risks, like bio-crimes (including bioterrorism), lead contamination with microorganism that needs decontamination. Decontamination is a critical point for controlling spread of microorganism. Many chemicals are used for decontamination where hydrogenperoxide (H2O2) is massively applied due to its antimicrobial properties. However, no applicable system found to screen the process of decontamination. We designed a bacterial microfluidic biosensor using Staphylococcus aureus, which is known to be catalase positive, to screen efficacy of H2O2 applications. In this study we are representing the microbial optimization part of the study with respect to drying process and validation of the process. The results showed that drying had no effect on the catalase reaction and retail decontaminants can be evaluated with this approach.

Anahtar Kelimeler

• Biorisk
• Staphylococci
• Decontamination
• Hydrogenperoxide
• Validation

Hidrojen peroksit dekontaminasyon etkinliğinin belirlenmesine yönelik mikrofluidik katalaz biyosensörü: Mikrobiyal optimizasyon

Öz

Biyolojik suçlar (biyoterörizm dahil) gibi biyolojik riskler, mikroorganizmaların bulaşmasına yol açarak dekontaminasyon gerektirmektedir. Dekontaminasyon, mikroorganizmanın yayılmasını kontrol etmek için kritik bir noktadır. Dekontaminasyon için birçok kimyasal kullanılmaktadır. Ancak ekonomik ve seçkin antimikrobiyal özelliklerinden dolayı hidrojenperoksitin (H2O2) uygulamasıda sıktır. Dekontaminasyon sürecini izlemek için uygun bir sistem bulunamamıştır. Bu çalışmada, H2O2 uygulamalarının etkinliğini taramak için katalaz pozitif olduğu bilinen Staphylococcus aureus kullanarak bir bakteriyel mikrofluidic (mikroakışkan) biyosensör tasarladık. Bu çalışmada öncelikle, kurutma prosesi ve prosesin validasyonu ile ilgili olarak çalışmanın mikrobiyal optimizasyon kısmı hazırlanmıştır. Sonuçlar, kurutmanın katalaz reaksiyonu üzerinde hiçbir etkisi olmadığını ve satışta bulunan dekontaminantların bu yaklaşımla değerlendirilebileceğini göstermiştir.

Anahtar Kelimeler

• Biorisk
• Staphylococci
• Decontaminasyon
• Hidrojenperoksit
• Validasyon

Kaynakça

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