Salmonella Gallinarum ve Salmonella Pullorum için qPCR Tanı Kitinin Geliştirilmesi

Yıl 2019, Cilt: 30 Sayı: 2, 137 - 142, 27.12.2019

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https://doi.org/10.35864/evmd.631225

Öz

Konak spesifik ve biovar olan Salmonella Gallinarum ve Salmonella Pullorum başta tavuk olmak üzere hindi, bıldırcın, güvercin, serçe ve papağanlarda önemli hastalıklara sebep olmaktadır. Bu nedenle kanatlı hayvanlarda bu serotipleri saptamak için hızlı ve güvenilir tanı yöntemleri hastalığın etkin kontrolü için gerekli olmaktadır. Bu amaçla biovarları saptamak ve birbirinden ayrımını sağlamak için eş zamanlı olarak S. Gallinarum/Pullorum ve internal kontrol hedefli reaksiyonları içeren hızlı qPCR tanı kiti geliştirildi. S. Gallinarum/Pullorum referans ve VKMAE Kanatlı Hastalıkları Laboratuvarı'ndan temin edilen S. Gallinarum suşlarına qPCR metodu uygulandı. Çalışmada qPCR analizinin duyarlılığı ve özgüllüğü değerlendirildi. Sonuçlar geliştirilen qPCR yönteminin S. Gallinarum/Pullorum'u  kesin olarak saptayabildiğini gösterdi. qPCR yönteminin kullanım kolaylığı, güvenirliliği, teknik olarak basit, tam otomasyon, spesifik, hassas, hızlı olmasından dolayı bu biovarların teşhisi ve ayrımında daha fazla avantaj sağlayacağı sonucuna varıldı.

Anahtar Kelimeler

Salmonella, qPCR, tanı

Development of qPCR Diagnostic Kit for Salmonella Gallinarum and Salmonella Pullorum

Öz

Salmonella Gallinarum and Salmonella Pullorum cause important disease of chicken, the others are turkey, quail, pigeon, sparrow and parrot, which are host specific and biovars of each other. Therefore, rapid and reliable meth-ods to detect these poultry-associated Salmonella serotypes are necessary for efficient control of Salmonella in poultry. For this purpose, a rapid qPCR diagnostic kit including S. Gallinarum / Pullorum and internal control targeted reactions was developed simultaneously to detect and differentiate biovars. S. Gallinarum/Pullorum reference and S. Gallinarum strains that were developed from VKMAE Poultry Laboratory were performed using qPCR method. In this study, sensitivity and specificity of qPCR analysis were evaluated. The results showed that the developed qPCR method was able to 

detect Salmonella Gallinarum/Pullorum correctly. Since the ease of use, reliability, technically simple, full automation, specific, sensitive and fast, the qPCR method is thought to provide more advantages in the diagnosis and differentiation of these biovars.

Kaynakça

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