Fluoreseent antibody technique

Yıl 1963, Cilt: 2 Sayı: 2, 112 - 122, 01.12.1963

Mediha Şentürk

Öz

A short history of the development of the ImmunochemichaJ
staining procedures and the imporlance of this method in diagnosis
have been presented.
The future significance of the fluorescent antibody tests in microbiology
in connection with the diagnosis and control of the contagious
diseases was discussed.
The nature and quantity of fluorescence and the selection of
the optical equipment have been discussed.
The types of fluorescent antibody tests which are in use have
been explained. The importance of the FA method in the rapid identification
of microorganisms was pointed out as opposed to the slow
standard tests which require a great amount of laboratory work.
The rapid and spesific way of identifying unknown antigen
and antibody through the FA method has been discussed.

Fluorescent antikor metodu

Öz

Immunochemichal boyama usullerinin tekamülü hakkında kısk
'hir tarihçe ile bu metodun teşhisteki önemi takdim edilmiştir. Fluorescent antikor testlerinin bulaşık hastalıklann teşhis ve
kontrolu ile ilgili olarak mikrobiyoloji sahasında gelecekteki önemi
münakaşa edildi.
Fluoı'escense'ın miktar ve tabiatı ile optik aletlerin seleksiyonundan
bahsedildL
Kullanılan fluorescent antikor testlerinin tipleri açıklandı ve
FA metodunun daha yavaş ve fazla laboratuvar çalışmasını icabettiren
standart testlere nazaran mikroorganizmlerin sür'atle idantifikasyonundaki büyük önemi belirtildi.
Bilinmeyen antijen ve antikodann FA metodu ile çabuk ve özel
olarak idantifikasyonlan münakaşa edildi.

Anahtar Kelimeler

Fluorescent antikor

Kaynakça

• 1 - Cherry, W. E., Goldman, M., and Carsld, T. R., with the collaboration ot Moody, M. (1960) Fluorescent Antibody Techniques in the Diagnosis of communicable diseases. United States Government Printlng office Washintong D. C. Public Health Service Publication No. 729.
• 2 - Communicable Disease Center Laboratory Branch Atlanta, Georgia. Training Course in Fluorescent Antibody Technique for Grouping Beta Hemolytic Steptococci. Lal:ıor:.ıtory Manual. (1961).
• 3 Coons A. H., and Kaplan, M. H. : Localization of antigen in tissuc cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody J. Exper. "Med. 91 : 1 - 13, 1950.
• 4 Cunningham, Charles H. (1960) A laboratory Guide in Virology.
• 5 Kaplan, M. H., Coons, A. H. and Deane, H. W. : Localization of Antigen in Tissue Cells. III - Celluler distribution of pneumococcal polysaccharides typesii and III in the mouse. J. Exp. Med. 91 : 15 - 30, 1950.
• 6 - Goldwasser R. A. and Shepard C .C. : Sta.ining of complement and modifications o! fluorescent antibody procedures. J. Immuno!. 80 : 122 - 131, 1958.
• 7 Moody, Max D .. Ellis, E. C. and Updyke, E. L. : Statining Bacterial Smears with Fluorescent antibody. VI - Ideni!ication of Salmonellae In Fecal Spccimens (REPRİNT)
• 8 - Pelczar, M. J. Jr. ,Reid, R. D. (1958) Microbiology.