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The investigation of Brucella canis antibodies by mercaptoethanol micro agglutination test in horses

Yıl 1987, Cilt: 6 Sayı: 1, 95 - 100, 01.12.1987

Öz

The Investigation of Brucella canis Antibodies by Mercaptoethanol Micro Agglutination Test in Horses. Sera of 78 horses were tested by mercaptoethanol micro agglutination test for agglutinins to Brucella canis. A titer of 1:16 or more in mercaptoethanol microagglutination test was considered as indicative of active infection. No sera were found to be positive at this titer. Of 78 sera investigated, 37.1per cent were positive at 1:4 titer and 16.6 per cent were positive at 1:8 titer. It was concluded that a survey which will be performed with numberous sera containing wide variety of horses may give more reliable results.

Kaynakça

  • 1 - BOSU, W.T.K. and PRESCOTT, J.F. (1980 : A serelogic survey dogs for Brucella canis in southwestern Ontario. Can. Vet. J., 21: 198-200.
  • 2 - CARMICHAEL, L.E. and JOUBERT, J.C. (1987) : A rapid slide agglutination test for the serediagnosis of Brucella canis infection that employs a v.ariant (M-) or.ganism as antigen. Cornell Vet.r 77: 3-12.
  • 3 - CURRIER, RW., RAITHEL, W.F., MARTIN, R.J. and POTTER, M :E. (1982) Canine brucellosis. J. Am. Vet. Med . . Assoc., 180: 132-133.
  • 4 - DENNY, H.R. (1972) : Brucellos'is in the horse. Vet. Rec., 90: 86-91.
  • 5 - DİKER, S., İSTANBULLUOGLU, E., AYHAN H. ve SOYSAL, G. (1984) Bursa bölgesindeki insanlarda Brucella canis infeksiyanları üzerinde serolojik bir inceleme. Mikrobiyel. Bült., 18: 203-207.
  • 6 - DİKER, K.S., AYDIN, N. ERDEGER, J. and ÖZYURT, M. (1987). A serelogic survey of dogs f.or Brucella canis and Brucella abortus and evaluation of mercapto ethanol microa·gglutination test. A.Ü. Vet. Fak. Derg., (Baskıda).
  • 7 - FLORES-CASTRO, A. and SEGURA, H, (1976) : A serological and bacteriological survey of canine br·ucellosis in Mexico. Cornell Vet., 65: 347-352.
  • 8 - HOFF, G.L. and NIGHOLS, J.B. (1974) : Canine brucellosis in Florida: selogic survey of pound dogs, animal shelter workers and veterinarians. ·Am. J. Epidemiol., 100: 35-39.
  • 9 - İSTANBULLUOGLU, E. ve DiKER, S. (1983) . Brucella canis üzerinde serolojik incelemeler. A.Ü. Vet. Fak. Der., 30: 1'4-18.
  • 10 - MACMILLAN A.P. and COCKREM, D.S. (1986) : Observations on the long term effects of Bruceila abortus infection in the horse, including effects during pregnancy and lactation. Equine Vet. J., 18: 388-390.
  • 11 - MONOROE, P.W., SILBEıRG, S.L. and MORGAN/ P.M. (1975). Seroepidell) iol-ogical investigaNon of Bruceila canis antibodies in different human population groups. J. Clin. Microbiol., 25 382.386.
  • 12 - Nicoletti, P.L. MıAHI..!ER J.R. and Scarratt, W.K. (1982) Study of agglutinins to Bruceila abortus, B canis and Actinobacillus equuli in horses. Equine Vet. J., 14: 302-304.
  • 13 - RANDHAWA, A.S., KELLY, V.P. and BAı~EH, E.·F. (1977) : Agglutinins to Coxiella burnetti and Brucella spp, with particular reference to Brucella canis, in wild animals of southern Texas. J. Am. Vet. Med. Assoc., 171: 939-942.
  • 14 - RANDHAWA, A.S., DlETERICH, W.H. and HUNTER, C.C. 1(1977) : Prevalence of seropositive reactions to Brucella canis in a limited survey of domestic cats. J. Am. Vet: Med. Ass oc , 171 : 267-268.
  • 15 - SAEGUSA, J., UEDA, K., GOTO, Y. and FUJIWARA, K. (1978). A survey (of Brucella canis infection in dogs from Tokyo area. Jap, J. Vet. Sci., . 40: 75-80.
  • 16 - SARD.M. (1978) : Expectation and observation. Vet. Rec., 103: 552-555.
  • 17 - ZOHA, S J. and CARMICHAEL, L.E. (1981) : Properties of Brucella canis surface antigens associated with colonial mucoidness. Cornell Vet., 71: 428-438.

Brucella Canis'e Karşı Olan Antikorların Atlarda Merkaptoetanol Mikroaglütinasyon Testi ile Araştırılması

Yıl 1987, Cilt: 6 Sayı: 1, 95 - 100, 01.12.1987

Öz

Toplam 78 atın serumları merkaptoetanol mikroaglütinasyon testi ile Br. Canis antikorları yönünden incelendi. Merkaptoetanol mikroaglütinasyon testinde 1:16 veya üstündeki sulandırmalarda pozitif reaksiyon veren serumlar aktif inkesiyonun belirtisi olarak kabul edildi. Bu titrede pozitif reaksiyon veren serum bulunmadı. İncelenen serumların %37.1'i 1:4 sulandırmada, % 16.6'sı 1:8 sulandırmada pozitif reaksiyon verdi. Daha çok sayıda örnek ile ve daha geniş kapsamlı bir at populasyonunu içine alan bir araştırmanın yararlı olacağı kanısına varıldı.

Kaynakça

  • 1 - BOSU, W.T.K. and PRESCOTT, J.F. (1980 : A serelogic survey dogs for Brucella canis in southwestern Ontario. Can. Vet. J., 21: 198-200.
  • 2 - CARMICHAEL, L.E. and JOUBERT, J.C. (1987) : A rapid slide agglutination test for the serediagnosis of Brucella canis infection that employs a v.ariant (M-) or.ganism as antigen. Cornell Vet.r 77: 3-12.
  • 3 - CURRIER, RW., RAITHEL, W.F., MARTIN, R.J. and POTTER, M :E. (1982) Canine brucellosis. J. Am. Vet. Med . . Assoc., 180: 132-133.
  • 4 - DENNY, H.R. (1972) : Brucellos'is in the horse. Vet. Rec., 90: 86-91.
  • 5 - DİKER, S., İSTANBULLUOGLU, E., AYHAN H. ve SOYSAL, G. (1984) Bursa bölgesindeki insanlarda Brucella canis infeksiyanları üzerinde serolojik bir inceleme. Mikrobiyel. Bült., 18: 203-207.
  • 6 - DİKER, K.S., AYDIN, N. ERDEGER, J. and ÖZYURT, M. (1987). A serelogic survey of dogs f.or Brucella canis and Brucella abortus and evaluation of mercapto ethanol microa·gglutination test. A.Ü. Vet. Fak. Derg., (Baskıda).
  • 7 - FLORES-CASTRO, A. and SEGURA, H, (1976) : A serological and bacteriological survey of canine br·ucellosis in Mexico. Cornell Vet., 65: 347-352.
  • 8 - HOFF, G.L. and NIGHOLS, J.B. (1974) : Canine brucellosis in Florida: selogic survey of pound dogs, animal shelter workers and veterinarians. ·Am. J. Epidemiol., 100: 35-39.
  • 9 - İSTANBULLUOGLU, E. ve DiKER, S. (1983) . Brucella canis üzerinde serolojik incelemeler. A.Ü. Vet. Fak. Der., 30: 1'4-18.
  • 10 - MACMILLAN A.P. and COCKREM, D.S. (1986) : Observations on the long term effects of Bruceila abortus infection in the horse, including effects during pregnancy and lactation. Equine Vet. J., 18: 388-390.
  • 11 - MONOROE, P.W., SILBEıRG, S.L. and MORGAN/ P.M. (1975). Seroepidell) iol-ogical investigaNon of Bruceila canis antibodies in different human population groups. J. Clin. Microbiol., 25 382.386.
  • 12 - Nicoletti, P.L. MıAHI..!ER J.R. and Scarratt, W.K. (1982) Study of agglutinins to Bruceila abortus, B canis and Actinobacillus equuli in horses. Equine Vet. J., 14: 302-304.
  • 13 - RANDHAWA, A.S., KELLY, V.P. and BAı~EH, E.·F. (1977) : Agglutinins to Coxiella burnetti and Brucella spp, with particular reference to Brucella canis, in wild animals of southern Texas. J. Am. Vet. Med. Assoc., 171: 939-942.
  • 14 - RANDHAWA, A.S., DlETERICH, W.H. and HUNTER, C.C. 1(1977) : Prevalence of seropositive reactions to Brucella canis in a limited survey of domestic cats. J. Am. Vet: Med. Ass oc , 171 : 267-268.
  • 15 - SAEGUSA, J., UEDA, K., GOTO, Y. and FUJIWARA, K. (1978). A survey (of Brucella canis infection in dogs from Tokyo area. Jap, J. Vet. Sci., . 40: 75-80.
  • 16 - SARD.M. (1978) : Expectation and observation. Vet. Rec., 103: 552-555.
  • 17 - ZOHA, S J. and CARMICHAEL, L.E. (1981) : Properties of Brucella canis surface antigens associated with colonial mucoidness. Cornell Vet., 71: 428-438.
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Cerrahi
Bölüm Araştırma Makaleleri
Yazarlar

Nejat Aydın Bu kişi benim

Jale Erdeğer Bu kişi benim

K. Serdar Diker Bu kişi benim

Yayımlanma Tarihi 1 Aralık 1987
Gönderilme Tarihi 1 Aralık 1987
Yayımlandığı Sayı Yıl 1987 Cilt: 6 Sayı: 1

Kaynak Göster

APA Aydın, N., Erdeğer, J., & Diker, K. S. (1987). Brucella Canis’e Karşı Olan Antikorların Atlarda Merkaptoetanol Mikroaglütinasyon Testi ile Araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 6(1), 95-100.


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