Türkiye'deki Koyunların Mavidil Hastalığının Serolojik, Etiyolojik ve Patolojik Durumu Üzerinde Araştırmalar

Yıl 1988, Cilt: 6 Sayı: 3, 13 - 24, 01.12.1988

Hamdi Girgin

Öz

Ege bölgesinde değişik odaklardaki hasta koyunlardan alınarak
OPG solusyonu içinde laboratuvara getirilen materyallerin 5
tanesinden kuzu .böbreği hücre kültürlerinde mavidil tip-4 virusu
izole edildi · Bu 5 virus suşu arasında serolojik bir farklılık bulunmadı.
Hastalıklı materyal önce deney koyunlarına verildi. Bu koyunlardan alınan defibrine kan 10 günlük embriyonlu yumurtanın sarı
kesesine 0,2 ml inokule edilip 33,5°C'lik etüve kondu. Üç-beş gün
arasında ölen embriyonlar toplanıp pasaj için kullanıldı. Embriyonlu
yumurtada 8 pasajdan sonra primer kuzu böbreği hücre kültürlerine
ekildi. Hücrelerden OPE meydana geldi. Spesifik antiserumla
yapılan SN testlerinde hücrelerde OPE şekillenmedi. Bu virusların doku kültüründe 12.nci pasajdaki titresi 10-5,25 ile 10-5,50.
TCID/50 ml. arasındaydı.
Teste tabi tutulan 691 adet çeşitli serumun 294 adedinden mavidil
tip-4 antikoru saptandı. Bu oran koyunlarda % 46, sığırlarda .
% 16 ve keçi serumunda da % 44 olarak bulundu.
Mavidili hastalığına yakalanmış 39 koyunda makroskopik ve
mikroskopik lezyonlar belıirlendi. Genellikle en belirgin olanlar yaygın ödem, hiperemi ve kanama; oral bölgede erozyonlar; akciğer
ve lenf düğümlerinde hiperemi ve ödem; iskelet ve kalp kaslannda
kanama idi.

Anahtar Kelimeler

Türkiye, Türkiyedeki Koyunlar, Koyun, Koyun Mavidil, Mavidil, Mavidil Hastalığı, Mavidil Hastalığının Serolojik Durumu, Mavidil Hastalığının Etiyolojik Durumu, Mavidil Hastalığının Patolojik Durumu, Mavidil Hastalığı Üzerinde Araştırmalar, Hamdi GİRGİN, A. Demir YONGUÇ, 1987, 1988, Etlik

(Serological, etiological and pathological studies on blutongue disease of sheep in Turkey)

Öz

Bluetongue virus type-4 was ilolated in the lamb kidney tissue culture from 5 of the materials taken in OPG solution from the animals suffering from the disease in the various faco of AEGEAN REGION.
The materials were inoculated into the experimental sheep. The defirinated blood collected from the diseased sheep were inoculated into the yolk sacs of the ten days old embryonated eggs in an amount of 0,2 ml and they were kept in an incubator at 33,5 C.
The embryos which died within 3-5 days were collected and used for passages. They were cultivated into primary lamb kidney tissue culture after eight passages in the embryonated eggs. The OPE was observed. No OPE was observed in the cells in SN test applied by using specific antiserum. The titer of this virus at he twelfth passage in the tissue culture was between 10-5,25 and 10-5,50 TCİD/50 ml.
Bluetongue type-4 antibody was determined in 294 blood sera of 691 various sera subjected to the test. This ratio was found to be 46% for sheep, 16 % for cattle and 44 %for goats.
Gross and microscopic lesions in 39 sheep infected with bluetongue were described. In general, the most consistent were generalized edema, hyperemia and hemorrhage; erosions in the oral region; pulmonary and lymphnode hyperemia and edema; and lymphnode hyperemia and edema; and hemorrhages of skeletal and cardiac muscles.

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