Haemophilus Somnus'un Biyolojik Materyaldeki Dayanıklığı

Yıl 1989, Cilt: 6 Sayı: 5, 127 - 136, 01.12.1989

K. Serdar Diker Hakan Yardımcı Kazunori Hashımoto

Öz

Sığır orijinli iki Haemophilus somnus suşunun kan, serum, vaginal mukus,süt, idrar .ve sofra içinde 4°C, 25°C ve 37"C'de canlı kalma
süreleri incelendi. infeksiyon kökenli suş 37"C'de kan ve serumda
59 günden fazla canlı kaldı. Vaginal floradan izole edilen suş ise
en uzun süre 37 derecedeki vaginal mukusta canlılığını korudu. Her iki
suş incelenen tüm ısılarda, safra ve idrarda kısa sürede öldü.

Anahtar Kelimeler

Haemophilus Somnus, K. Serdar DİKER, Hakan YARDIMCI, Kazunori HASHIMOTO

Survival of Haenıophilus sommus in biological milleus

Öz

Survival . of two Haemophilus strains in blood, serum, vaginal
mucus, milk, urine and bile at different temperatures was investigated.
H.somnus strain isolated from a elinical case survived for
more than 59 days in blood and serum at 37"C. H.somnus isolated
from normal vaginal flora survived longest (24 days) in vaginal
mucus at 37"C. Survival was four days or less in all cases when the
organisms were' mixed with bile and urine.

Kaynakça

• ARDA. M., DiKER , K.S., ERDEGER:, J. (1989) : The comparison of three serological test in the diagnosis of H:somnus infection in cattle. DOGA Turk Vet. Hayv. Derg. (In press) .
• ARMSTRONG. K.R., OSBORNE. AD., JANZEN , E.D. {1986) : Haemophilus somnus mastitis in a ·dairy crYW. Can. Vet. J., 27: 211-212.
• CORIBEIL, L.B., BL.A!U, K., PRIEUR, D.J., WARO, AC.S. (1985) : Serum susceptibility of Haemophilus somnus from bovine elinical cases and carrlers.J. Clin. Microb., 22: 192-198. ·
• CORBEIL, L.B., WIDDERS, P.R., GOGOLEWSKI, R., ARllHUR, J.. INZANA. T.J., WARD, A.C.S. (.1966) : ıHaemophilus somnus: bovine reproductive and respiratory disease. Can. Vet. J., 27ı: 90-93.
• CRANDELL, R.A., SMITıH, AR., KI·SSIL, M. (1977) : Colonization and trans-.mission of Haemophilus somnus in cattle. Am. J. Vet. Res., 38: 1749-1751.
• DEWEY, K.J., LITTLE, P.B. {1984) : En;v:ironmental survival of Haemophilus somnus and influence of secretions i:ınd excretions. Can. J. Comp. Med.,. 48: 23-26.
• DiKER, K.S., - ARDA, M .iZGÜR, H. (1986) : Isolation and characetrization of .Haemop'hilus somnus .fro.m cows wlth metritis. A.Ü. Vet. Fak. ıOerg . , 33:52-57.
• GOGOLEWSKI, R.P., LEATHERS, C.W., UGGITT, H.D., CORIBEIL, L.B. (1987): Experimental Haemophilus somnus pneumonia in calves and immunoperoxidase localization of bacteria. Vet. Pathol.. 24: 250-256.
• GROOM, S.C .. LITTLE, P.B., ROSENDAL , S. (1988) : Virulence differences among three strains of Haemophilus somnus following intratraoheal inoculation of calves. Can. J. Vet. Res., 52: 349-354.
• HAZLETT, M.J., LITTLE, P.B., BARNUM, D.A. {1983) : ·Experimental production of mastitis with Haemophilus somnus in the lactating 'bovine mammary gland. Can. Vet. J., 24: 135-136.
• HUMPHREY, J.D., STBPHENS, L.R. (1983) Haemophilus somnus: a review. Vet. Bull., 53: 987-1003.
• HUMHREY, J.D., LITTLE, P.B .. STEP.H5NS, L.R .. BARNUM, D.A., DOIG, P.A.,THORSEN, J. {1982) : Prevalence and distribution of Haemophilus somnus in the male bovine reproductive tract. Am. J. Vet. Res., 43: 791-795.
• KIUPEL, H., PREHN, ı. (1986) : Zur .Haemophilus somnus infektion des rindes bakteriologische untersuchungsergebnisse unter besanderer berücksictigung von abortsulbstraten. Aroh. E~per. Vet. Med., 40: 164-.169.
• LITILE, P.B. {1986) : Haemophilus somnus complex: patheçenesis of the septicemic thrombotic meningoencephalitis. Can. Vet. J ., 27": 94-96.
• MILES, A.A., MISRA, oS.S. (1938) : lihe estimation of the bacteriocidal power of the ıblood. J . Hyg., 38: 732-749.
• MILLER. R.B., BARNUM, DA, McENTEE. K.E. {1983) : Haemophilus somnus in the reproductive tract of slaughtered cows: location and frequency of isolations and · lesions. Vet. Path ol. 20: 515-521 .
• MILLER, R.!3., LEIN, D.H., McENTEE, K.E., HALL, C.E., SHIN, S, (1983) : Haemophilus ısomnus infection of the reproduotive tract of cattle: a review. J. Am. Vet. ıMed . Assoc., 1"82: 1390-1392.
• PATIERSON, R.M., MITCHELL, G.ıM.. HUMRHREY, J.D., STEPHENS, L.R. {1986) : Experimental induction of vaginitis in heifers 'by infection with Haemophilus somnus. Aust. Vet. J ., 63: 163-1-65.
• SIMONSON. R., MAJHESWARAN, S.K., WARD, G.E. (1981) : Radiolabeled . substrate. assa·y to measure in'hibition of growth of Haemophilus sam n us by normal bovine serum. Am. J . Vet. Res., 42:. 1235-1237.
• STEPHENS, L.R., LITTLE, P.B., WIUKI·E. B.N., 'BARNUM, D.D. {1981) : lnfectious thromboembolic meningoencep'halitis in cattle: a review. J. Am. Vet. Med. Assoc., 178: 378-384.
• WIDDEHS. P.R., PAISLEY, L.G., GOGOLEWSKI , R.P., EVERMANN, J.F., SMITH, J.W., CORBEIL, UB. (1986) : Experimental a'bortion and the systemic immune response to Haemophilus somnus in cattle. lnfect. lmmun., 54: 555 -'560.